[1]陈韵,石展望,黄晓敏.超声波辅助提取大豆总木脂素及其含量分析[J].大豆科学,2010,29(01):168-170,173.[doi:10.11861/j.issn.1000-9841.2010.01.0168]
　CHEN Yun,SHI Zhan-wang,HUANG Xiao-min.Ultrasonic-assisted Extraction of Total Lignans and Its Content Analysis inSoybeans[J].Soybean Science,2010,29(01):168-170,173.[doi:10.11861/j.issn.1000-9841.2010.01.0168]
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超声波辅助提取大豆总木脂素及其含量分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年01期 页码: 168-170,173 栏目: 出版日期: 2010-02-25
Title:
Ultrasonic-assisted Extraction of Total Lignans and Its Content Analysis inSoybeans
文章编号:
1000-9841（2010）01-0168-03
作者:
陈韵1; 石展望2; 黄晓敏1
1.韶关学院 生物科学系，广东 韶关 512005；
2.广西民族大学 化学与生态工程学院，广西 南宁 530006
Author(s):
CHEN Yun1; SHI Zhan-wang2; HUANG Xiao-min1
1.Bioscience Department of Shaoguan University，Shaoguan 512005，Guangdong;
2.Chemical and Ecological Engineering College of Guangxi Ethnic University，Nanning 530006，Guangxi，China
关键词:
大豆; 总木脂素; 超声波辅助提取; 紫外-可见分光光度法
Keywords:
Soybean; Total lignans; Ultrasonic-assisted extraction; UV-Vis
分类号:
TS214.2
DOI:
10.11861/j.issn.1000-9841.2010.01.0168
文献标志码:
A
摘要:
以乙醇为浸提溶剂，超声波辅助提取不同品种大豆中的木脂素，并用紫外-可见分光光度法对其含量进行分析，同时对浸提溶剂、料液比、浸提时间及次数等因素进行比较。结果表明：以乙醇为浸提溶剂，料液比在1/8~1/12范围，浸提时间20 min，浸提2次效果比较理想；标准对照品在0~0.18 mg·mL-1浓度范围内吸光度与其总木脂素含量呈良好的线性关系，回归方程A=6.081c+0.0445，相关系数r=0.9998，总木脂素检出限8.3×10-5mg·mL-1，平均回收率为94.4%，RSD为1.2%。该方法简便、实用、可靠，可对大豆木脂素的深入研究提供参考。
Abstract:
Total lignans in several soybean samples were detected by UV-Vis with the ultrasonic-assisted extraction in this paper.The result showed that by twice extraction it can get ideal effect with ethanol as extracting reagent in the material/reagent ratio of 1/8~1/12.The relationship between standard substance concentration within 0~0 18 m·mL-1 and absorbance remained better linear.The regression equation was A=6.081c+0.0445,with the correlation coefficient r=0.9998，the detection limit was 8.3×10-5 m·mL-1with the average recovery of 94.4% and the RSD of 1.2%.Results suggest the method is convenient and practical to detect lignans from soybean.

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备注/Memo

基金项目：广东省科技计划资助项目（2005B33001004）

第一作者简介：陈韵（1965-），男，硕士，研究方向为生物无机化学。E-mail: yunchen68@163.com。

更新日期/Last Update: 2014-09-13