[1]李建东,燕雪飞,董思言,等.辽宁省野生大豆种质资源的SSR遗传多样性分析[J].大豆科学,2010,29(01):28-32.[doi:10.11861/j.issn.1000-9841.2010.01.0028]
　LI Jian-dong,YAN Xue-fei,DONG Si-yan,et al.Analysis of Genetic Diversity of Glycine soja Germplasm Resources in Liaoning Province[J].Soybean Science,2010,29(01):28-32.[doi:10.11861/j.issn.1000-9841.2010.01.0028]
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辽宁省野生大豆种质资源的SSR遗传多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年01期 页码: 28-32 栏目: 出版日期: 2010-02-25
Title:
Analysis of Genetic Diversity of Glycine soja Germplasm Resources in Liaoning Province
文章编号:
1000-9841（2010）01-0028-05
作者:
李建东; 燕雪飞; 董思言; 魏苗; 王国骄; 孙备
沈阳农业大学 农学院，辽宁 沈阳 110161
Author(s):
LI Jian-dong; YAN Xue-fei; DONG Si-yan; WEI Miao; WANG Guo-jiao; SUN Bei
College of Agronomy，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
野生大豆; 遗传多样性; SSR; 聚类分析
Keywords:
Glycine soja; SSR; Genetic diversity; Genetic distance; Cluster analysis
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2010.01.0028
文献标志码:
A
摘要:
以30份2007年辽宁省的野生大豆种质资源为材料，利用40对SSR引物进行遗传多样性分析。结果表明：18对SSR引物扩增出129个等位变异，平均每个位点等位变异7.22个，Shannon-Weaver指数变化范围为1.1753~2.1234，平均为1.7285。中部平原半湿润区内的种质数、平均等位变异数和遗传多样性指数最高，其次为东部山地湿润区，西部丘陵半干旱区内分布种质数最少，其平均等位变异数和遗传多样性指数均最低。中部平原半湿润区和东部山地湿润区之间的遗传相似性最高（0.6496），遗传距离最近（0.4314），而西北部平原低丘半湿润区和西部丘陵半干旱区之间的遗传相似性最低（0.4326），遗传距离最远（0.8379）。聚类结果看到SSR分子标记的结果与品种的地理来源没有明显的相关性。
Abstract:
In order to evaluate the genetic diversity of the wild soybean(Glycine soja) in the different region of Liaoning Province，China，a total of 30 wild soybean accessions from 5 ecological regions(semi-humid zone of plain in the centre，semi-humid zone of low hill in the south，semi-humid zone of plain and low hills in the northwest，semi-arid zone of hill in the west ，and humid zone of mountainous lands in the east，abbreviated as regionⅠ，Ⅱ， Ⅲ， Ⅳ andⅤ，respectively )of Liaoning province in 2007 were genotyped using 40 pair of SSR primers.129 alleles were detected by 18 pair of SSR markers，with an average of 7.2 alleles for each locus.Shannon-Weaver diversity indexes varied from 1.1753 to 2.1234 with averaged 1.7285.The region Ⅰ had the most germplasmas，the alleles and the highest genetic diversity，followed by the region Ⅴ and the region Ⅳ had the least and the lowest.The value of genetic similarity was highest(0.6496)while the value of genetic distance was the lowest(0.4314)between the region Ⅰ and Ⅴ.The value of genetic similarity was the lowest(0.4326)while the value of genetic distance was highest(0.8379)between the region Ⅲ and Ⅳ.There was no relativity between the results by the SSR markers and geographic origin by cluster analysis.

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备注/Memo

基金项目：辽宁省教育厅高等学校创新团队科研资助项目（2009t088）。

第一作者简介：李建东(1964-)，男，教授，博士生导师。研究方向为农业生态，野生资源利用与保护。

更新日期/Last Update: 2014-09-13