YU Yan,JIANG Wei,TANG Jing-xian,et al.Electronic Mapping and Structure Analysis of Key Enzyme Genes on Aspartic Acid Metabolic Pathway in Soybean[J].Soybean Science,2010,29(01):22-27.[doi:10.11861/j.issn.1000-9841.2010.01.0022]
大豆天冬氨酸代谢途径关键酶基因电子定位与结构分析
- Title:
- Electronic Mapping and Structure Analysis of Key Enzyme Genes on Aspartic Acid Metabolic Pathway in Soybean
- 文章编号:
- 1000-9841（2010）01-0022-06
- Keywords:
- Soybean; Aspartic acid; Key enzyme genes; Electronic mapping; Gene structure
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 运用电子定位的方法，利用大豆基因组物理图和遗传图的整合图谱，完成了17个大豆天冬氨酸代谢途径中关键酶基因的定位以及结构分析。结果表明：这些酶基因分别定位在A1、B2、D1a、D1b、D2、F、G、H、J、L和N等11个连锁群上，并获得了相应连锁群区间两侧的标记；在基因结构分析上，AHAS、DHDPS、HK与TS没有内含子，DHAD基因的内含子数目最多，为13个，AK基因有12个内含子。通过定位获得的对应分子标记可以为基因分子辅助育种奠定基础，而基因结构信息能更好的实现基因功能分析。
- Abstract:
- The nutritive value of soybean mainly focused on the aspartate-derived amino acids，and the content of these amino acids were affected by the key enzymes gene from aspartic acid metabolic pathway.In this research，the integrated map between soybean public genetic maps and physical maps was used，and the key enzyme gene sequences were blasted with soybean genome database by local Blast software.Seventeen key enzyme genes in aspartic acid metabolic pathway of soybean were mapped on soybean genetic map，and gene structures were analyzed firstly.The result indicated that these enzyme genes were mapped on 11 linkages group，including A2，B2，C2，D1b，D2，G，I，L，and M，respectively，and the flanking markers of these genes on the linkage group were obtained.The number of introns was from 0 to 13.There was no intron in AHAS，DHDPS，HK，and TS，otherwise there were 13 introns in DHAD，and 12 introns in AK.The corresponding markers obtained from mapping were the base of molecular assisted selection，while the structure information can make gene function analysis easily.
参考文献/References:
[1]Yager T，Niekerson D A，Hood L E.The human genome Projeet：creating an infrastructure for biology and medicine[J].Trends in Biochemical Sciences，1991，16(12)：454-458. [2]Peakall D，Shugart L.The human genome projcet(HGP)[J].Ecotoxicology Springer，2002，11(l)：7-9. [3]于妍，宋万坤，刘春燕，等.植物天冬氨酸代谢关键酶基因研究进展[J].生物技术通报，2008，（增刊）：7-11.（Yu Y，Song W K，Liu C Y，et al.Research development of key enzymes gene on aspartic acid metabolic pathway in plants[J].Biotechnology Bulletin，2008，(Supplement):7-11.） [4]Qi Z M，Li H，Wu Q，et al.An integrated map of soybean physical map and genetic map[J].Journal of Northeast Agriculture University，2009，16(2)：12-16. [5]于妍.大豆天冬氨酸代谢相关酶基因克隆与定位分析[D].哈尔滨：东北农业大学，2009：26 .(Yu Y.Cloning and mapping analysis of related enzymes genes on aspartic acid metabolism soybean[D].Harbin：Northeast Agricultural University，2009：26.) [6]Hughes C A，Gebhardt J S，Reuss A，et al.Identification and expression of a cDNA encoding cystathionine-gamma-synthase in soybean[J].Plant Science，1999,146:69-79. [7]Silk G W，Matthews B F，Somers D A，et al.Cloning and expression of the soybean DapA gene encoding dihydrodipicolinate synthase[J].Plant Molecular Biology，1994，26(3)：989-993. [8]Brummer E C，Nickell A D，Wilcox J R，et al.Mapping the Fan locus controlling linolenic acid content in soybean oil[J].Joural of Heredity，1995，86:245-247. [9]Panthee D R，Pantalone V R，Saxton A M，et al.Genomic regions associated with amino acid composition in soybean[J].Molecular Breeding，2006，17：79-89. [10]Yu Y G，Saghai Maroof M A，Buss G R，et al.RFLP and microsatellite mapping of a gene for soybean mosajc virus resistance[J].Phytopathology，1994，84：60-64. [11]Mansur L，Lark K G，Kross H，et al.Interval mapping of quantitative trait loci for reproductive，morphological and seed traits of soybean(Glycine max?L)[J].Theoretical and Applied Genetics，1993，86：907-913. [12]Conclbido V C，Denny R L，Boutin S R，et al.DNA Marker analysis of loci underlying resistance to soybean cyst nematode(Heterodera glycine Ichinohe)[J].Crop Science，1994，34：240-246. [13]Song Q J，Marek L F，Shoemaker R C，et al.A new integrated genetic linkage map of the soybean[J].Theoretical and Applied Geneties，2004，109：122-128. [14]Choi Y，Hyten D L，Matukumalli L K，et al.A soybean transcript map：Gene distribution，haplotype and single-nucleotide polymorphism analysis[J].Genetics Society of America，2007，176：685-696.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
基金项目：引进国际先进农业科学技术计划资助项目（2006-G1(A)）；国家高技术研究发展计划资助项目（2006AA10Z1F4）；黑龙江省博士后科研启动基金资助项目(LHK-04014)。