[1]韩阳,曹永强,赖冰冰.植物抗病基因工程育种策略及其在大豆上的应用[J].大豆科学,2009,28(06):1103-1107.[doi:10.11861/j.issn.1000-9841.2009.06.1103]
　HAN Yang,CAO Yong-qiang,LAI Bing-bing.Strategies of Genetic Engineering Breeding for Plant Disease Resistance and Its Application in Soybean[J].Soybean Science,2009,28(06):1103-1107.[doi:10.11861/j.issn.1000-9841.2009.06.1103]
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植物抗病基因工程育种策略及其在大豆上的应用

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年06期 页码: 1103-1107 栏目: 出版日期: 2009-12-25
Title:
Strategies of Genetic Engineering Breeding for Plant Disease Resistance and Its Application in Soybean
文章编号:
1000-9841(2009)06-1103-05
作者:
韩阳1; 2; 3; 曹永强2; 赖冰冰3
1.沈阳农业大学 农学院，辽宁 沈阳 110161；
2.辽宁省农业科学院 作物研究所，辽宁 沈阳 110161；
3.辽宁大学 生命科学院，辽宁 沈阳
Author(s):
HAN Yang1; 2; 3; CAO Yong-qiang2; LAI Bing-bing3
1.College of Agronomy，Shenyang Agricultural University，Shenyang 110161，Liaoning；
2.Liaoning Academy of Agricultural Science，Shenyang 110161，Liaoning；
3.School of Life Science，Liaoning University，Shenyang 110036，Liaoning，China
关键词:
大豆; 基因工程; 抗病育种
Keywords:
Soybean[Glycine max（L.）Merrill]; Genetic engineering; Disease resistant breeding.
分类号:
S565.103.53
DOI:
10.11861/j.issn.1000-9841.2009.06.1103
文献标志码:
A
摘要:
概述了植物抗真菌病害、细菌病害和病毒病害基因工程育种的主要策略，重点介绍了大豆转基因抗病育种的主要成果。植物抗病害转基因育种策略主要包括基于寄主病原菌相互识别和信号传导体系的基因工程和基于抗菌蛋白的基因工程；植物抗病毒病害基因工程育种策略还包括病毒外壳蛋白基因介导的抗性、复制酶介导的抗性、运动蛋白介导的抗性、卫星RNA介导的抗性、RNA介导的抗性等。
Abstract:
This paper reviewed strategies in disease resistance breeding by genetic engineering.Then the research progress on disease-resistant breeding of soybean by transgenic technology was summarized.The breeding strategies for disease resistance with genetic engineering can be grouped into two general categories：(1）The expression of genes that participate in the host-virus interaction or signal transduction of the hypersensitive response.(2）The expression of gene products that are directly toxic to pathogens or that reduce their growth.These include pathogenesis-related proteins（PR proteins)，antifungal proteins，antimicrobial peptides，ribosome inactivating proteins（RIP)，and phytoalexins.Moreover，virus-resistant transgenics can be developed by introducing either viral coat protein（CP)or replicase gene encoding sequences.RNA-mediated resistance，known as post-transcriptional gene silencing，is a successful strategy.Other approaches for achieving viral resistance described in the article，include the use of satellite RNA and viral movement protein.

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备注/Memo

基金项目：国家科技攻关资助项目(2003ba538e)。

第一作者简介：韩阳（1962-），女，博士，研究方向为植物分子生物学。 E-mail: hanyang_0802@163.com

更新日期/Last Update: 2014-09-12