[1]王桃云,王莹,钱玮,等.大豆荚壳异黄酮制备与体外抗氧化性研究[J].大豆科学,2009,28(05):913-916.[doi:10.11861/j.issn.1000-9841.2009.05.0913]
　WANG Tao-yun,WANG Ying,QIAN Wei,et al.Preparation and Antioxidant Activity of Isoflavones from Soybean Hull in vitro[J].Soybean Science,2009,28(05):913-916.[doi:10.11861/j.issn.1000-9841.2009.05.0913]
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大豆荚壳异黄酮制备与体外抗氧化性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年05期 页码: 913-916 栏目: 出版日期: 2009-10-25
Title:
Preparation and Antioxidant Activity of Isoflavones from Soybean Hull in vitro
文章编号:
1000-9841(2009)05-0913-04
作者:
王桃云1; 2 ; 王莹2; 钱玮2 ; 陈鹏1
1.扬州大学 生物科学与技术学院，江苏 扬州 225001；
2.苏州科技学院 化学与生物工程学院，江苏 苏州 215009

Author(s):
WANG Tao-yun1; 2;  WANG Ying2; QIAN Wei2; CHEN Peng1
1. College of Biological Science and Technology，Yangzhou University，Yangzhou 225001，Jiangsu
2.College of Chemical and Biological Engineering，Suzhou Science and Technology University，Suzhou 215009，Jiangsu，China
关键词:
大豆荚壳; 异黄酮; 抗氧化活性; 清除自由基
Keywords:
Soybean hull; Isoflavones; Antioxidant activity; Scavenging capacity to radical
分类号:
Q944.42
DOI:
10.11861/j.issn.1000-9841.2009.05.0913
文献标志码:
A
摘要:
研究大豆荚壳异黄酮（Isoflavones from soybean hull，ISH）制备与体外抗氧化性作用。通过超声回流法提取和大孔吸附树脂纯化，得到纯化的ISH。以VC、BHT、芦丁和槲皮素为阳性对照，测定了ISH的还原力;对羟基自由基、超氧阴离子自由基、烷基自由基的清除率以及抑制油脂过氧化的能力。结果表明：ISH的还原力高于BHT和VC，ISH对这几种自由基均有一定的清除作用，ISH的添加量在试验范围内与其抗氧化活性呈正相关。当试验量大于5 μg.mL-1时，ISH的抗氧化性能均优于VC、BHT、芦丁和槲皮素。
Abstract:
To study isoflavones from soybean hull(ISH) preparation the antioxidant activities of ISH in vitro.The ISH was extracted by method of ultrasonic and reflux extraction and purified by macroporous adsorption resin.The reduction and the scavenging capacities on hydroxyl free radical，superoxide anion radical and alkyl radical as well as inhibition of peroxide value of the ISH were detected while the VC(antiscorbutic vitamin)，BHT(2，6-Di-tert-butyl-4-methylphenol)，quercetin and rutin flavonoids were used as positive control.The result showed，the ISH had higher reduction energy than BHT and VC，and had some scavenging ability on all radical.The amount of ISH used was in direct proportion to the antioxidation.The scavenging capacities of ISH was higher than the VC，BHT，quercetin and rutin flavonoids while the dosage was more than 5 μg.mL-1

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备注/Memo

作者简介：王桃云，（1973-）男，讲师，在读博士研究生，现主要从事天然产物研究与开发。E-mail：wangtaoyun@mail.usts.edu.cn。

通讯作者：陈鹏，教授，博士生导师。

更新日期/Last Update: 2014-09-18