[1]刘永鑫,韩英鹏,常玮,等.一种适合大豆MicroRNA鉴定的RT-PCR方法[J].大豆科学,2009,28(04):600-604.[doi:10.11861/j.issn.1000-9841.2009.04.0600]
　LIU Yong-xin,HAN Ying-peng,CHANG Wei,et al.A RT-PCR Method Suitable for MicroRNA Identification of Soybean[J].Soybean Science,2009,28(04):600-604.[doi:10.11861/j.issn.1000-9841.2009.04.0600]
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一种适合大豆MicroRNA鉴定的RT-PCR方法

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年04期 页码: 600-604 栏目: 出版日期: 2009-08-25
Title:
A RT-PCR Method Suitable for MicroRNA Identification of Soybean
文章编号:
1000-9841(2009)04-0600-05
作者:
刘永鑫1; 韩英鹏1; 常玮1; 邹权2; 郭茂祖2; 李文滨1
1东北农业大学大豆研究所，大豆生物学教育部重点实验室，黑龙江 哈尔滨 150030；
2哈尔滨工业大学，计算机科学与技术学院，黑龙江 哈尔滨 150001
Author(s):
LIU Yong-xin1; HAN Ying-peng1; CHANG Wei1; ZOU Quan2; GUO Mao-zu2; LI Wen-bin1
1Soybean Institute，Northeast Agricultural University；Chinese Education Ministry’s Key Laboratory of Soybean Biology，Harbin 150030，Heilongjiang
2.Department of Computer Science and Technology，Harbin Institute of Technology，Harbin 150001，Heilongjiang，China
关键词:
大豆; MicroRNA;  RT-PCR; 鉴定
Keywords:
Soybean; MicroRNA; RT-PCR; Identification
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0600
文献标志码:
A
摘要:
MicroRNAs(miRNAs)是一类调控真核基因转录后表达的非编码小分子RNA。绝大多数miRNAs由计算机预测得到，其存在与否及时空分布还不清楚。因此建立高效、敏感和特异的检测方法，是探明miRNAs在多种组织中时空调控的基础和前提。研究以Lau等和Shi等的方法为基础设计方案，采用通用反转录引物，以大豆幼叶、老叶、根、茎组织为研究材料，对预测的、且Sanger Institute公布的miR156a进行扩增以检测扩增效率和准确性。经比较，该方法具有反转录引物通用、成本低，操作简单，可快速、敏感、高通量地同时比较一种组织中多个miRNAs，也可比较多个组织中miRNA；且该方法比Northern Blot和Microarray需要的RNA量少得多。
Abstract:
The microRNAs (miRNAs) are a newly identified class of small non-coding RNA，which regulate post-transcriptional gene expressing in eukaryote.Most miRNAs are predicted through the computer，the presentence/absence and the spatial/temporal of miRNAs still unclear.It is important for the establishment of efficient，sensitive and specific detection methods to detect miRNAs in different organism or period.In this study，the protocol derived from Lau et al.and Shi et al.was used to detect miR156a，predicted and released by Sanger Institute，in old or young leaf，root，and shoot for verifying this method.By comparison，this method is low cost，simple，fast，and sensitive，common for primer of RT-PCR，which could identify a number of miRNAs in the same organism and compare miRNAs from different organism.Furthermore，this method need less RNA，in comparison with Northern Blot and Microarray.

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备注/Memo

基金项目：国家高技术研究发展计划（863计划）目标导向资助项目(2006AA10ZF1Z1)；国家高技术研究发展计划（863计划）重点资助项目(2006AA100104-4)。?

作者简介：刘永鑫 (1987-)，男，硕士研究生，研究方向为大豆生物技术、大豆生物信息学。
通讯作者：李文滨，教授，博士生导师。E-mail：wenbinli@yahoo.com。

更新日期/Last Update: 2014-09-21