[1]赵鹂,陆开形,朱诚.超干处理对大豆种子抗老化能力及抗氧化代谢的影响[J].大豆科学,2009,28(03):450-455.[doi:10.11861/j.issn.1000-9841.2009.03.0450]
　ZHAO Li,LU Kai-xing,ZHU Cheng.Effects of Ultra-dry Treatment on Anti-aging Ability and Antioxidative Metabolism of Soybean Seed[J].Soybean Science,2009,28(03):450-455.[doi:10.11861/j.issn.1000-9841.2009.03.0450]
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超干处理对大豆种子抗老化能力及抗氧化代谢的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年03期 页码: 450-455 栏目: 出版日期: 2009-06-25
Title:
Effects of Ultra-dry Treatment on Anti-aging Ability and Antioxidative Metabolism of Soybean Seed
文章编号:
1000-9841(2009)03-0450-06
作者:
赵鹂1; 3; 陆开形2; 朱诚3
1丽水学院，浙江 丽水 323000；
2宁波大学生命科学与生物工程学院，浙江 宁波 315211；
3浙江大学生命科学学院，浙江 杭州 310029
Author(s):
ZHAO Li1; 3; LU Kai-xing2; ZHU Cheng3
1Lishui University，Lishui 323000，Zhejiang；
2?Faculty of Life Science and Biotechnology，Ningbo University，Ningbo 315211，Zhejiang；
3College of Life Science，Zhejiang University，Hangzhou 310029，Zhejiang，China
关键词:
大豆; 种子超干; 人工老化; 抗氧化代谢
Keywords:
Soybean; Seed ultradry; Artifical aging; Antioxidative metabolism
分类号:
S565.1;S330.3+1
DOI:
10.11861/j.issn.1000-9841.2009.03.0450
文献标志码:
A
摘要:
通过控制超干处理时间获得不同含水量的大豆种子（8.1%、4.0%和2.4%），对这些不同超干种子进行老化及回水处理，同时测定其抗老化能力及抗氧化酶等生理指标。结果发现：超干处理能提高大豆种子的抗老化能力，延长种子的贮藏寿命。超干种子(MC4.0%和2.4%)内氧自由基水平高于未超干种子(MC 8.1%)，但与老化前相比，未超干老化种子内氧自由基和丙二醛含量（MDA）的增加幅度均明显大于超干老化的种子，经超干老化种子的氧自由基清除能力要高于未超干老化的种子。超干种子的较强的抗老化能力还与其相对较高的抗氧化酶(SOD、POD、CAT)系统有关，发现老化导致超干种子的种胚和子叶中SOD、POD和CAT活性的下降要小于未超干种子的下降幅度。结果认为抗膜脂过氧化能力的保持是超干种子耐贮藏性的生理原因之一。
Abstract:
Soybean seeds with different water content(（8.1%、4.0% and 2.4%）were obtained by controlling ultra-drying time，then those seeds were treated by aging and re-water，those parameters such as anti-aging ability and antioxidant enzymes were measured.Results showed that ultradrying treatments can improve the ability of aging-resistance，thus prolonging its storage longevity.The free radical content was higher in ultradrying seeds(moisture content 4.0% and 2.4%，respectively）than that of non-ultradrying seeds(moisture content 8.1%).But compared with pre-aging seeds，the increasments of free radical and malonaldehyde(MDA)content in aged non-ultradrying seeds were significantly higher than that in ultradrying seeds，while the activity of lipoxygenase(LOX) in aged ultradrying seeds were evidently higher than that in aged non-ultradrying seeds.The higher ability of aging-resistance in ultradrying seeds was also attribute to its higher antioxidative system including superoxide dismutase（SOD)，peroxidase(POD) and catalase（CAT).The decrease in SOD，POD and CAT activity induced by aging was lower in ultradrying seeds compared with non-ultradrying seeds.Results suggest that the maintenance of the ability of resisting the superoxidation of the membrane lipids would be one of the reasons to prolong storage longevity of ultradried seeds.

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备注/Memo

基金项目：国家自然科学基金资助项目(N0301705950)。

作者简介：赵鹂，女，（1964-），硕士，副教授，研究方向为植物生理学。E-mail:xyzhaoli@126.com。

更新日期/Last Update: 2014-09-25