[1]寇坤,刘丽君,曲姗姗,等.大豆新品系黑农56子叶节再生体系的优化[J].大豆科学,2009,28(03):400-402,408.[doi:10.11861/j.issn.1000-9841.2009.03.0400]
　KOU Kun,LIU Li-jun,QU Shan-shan,et al.Improvement of Regeneration System in New Soybean Line Heinong 56[J].Soybean Science,2009,28(03):400-402,408.[doi:10.11861/j.issn.1000-9841.2009.03.0400]
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大豆新品系黑农56子叶节再生体系的优化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年03期 页码: 400-402,408 栏目: 出版日期: 2009-06-25
Title:
Improvement of Regeneration System in New Soybean Line Heinong 56
文章编号:
1000-9841(2009)03-0400-03
作者:
寇坤1; 2; 刘丽君2; 曲姗姗1; 2; 唐晓飞2; 杨喆2
1东北农业大学研究生院，黑龙江 哈尔滨 150030；
2黑龙江省农业科学院大豆研究所，黑龙江 哈尔滨 150086
Author(s):
KOU Kun1; 2; LIU Li-jun2; QU Shan-shan1; 2; TANG Xiao-fei2; YANG Zhe2
1Graduate School of Northeast Agricultural University，Harbin 150030，Heilongjiang;
2Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆; 子叶节; 再生
Keywords:
Soybean; Cotyldonary nodes; Regeneration
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.03.0400
文献标志码:
A
摘要:
为获得高效的大豆再生体系，选用大豆新品系黑农56子叶节进行了组织培养的研究，确定黑农56最适的萌发培养基与芽诱导培养基激素浓度。即萌发培养基为不含6-BA的MS培养基；芽诱导培养基添加6-BA和IBA的浓度分别为2.0 mgL-1，0.2 mgL-1的B5培养基。并确定黑农56的草铵膦选择压力为4 mgL-1?。
Abstract:
In order to acquire high efficiency soybean regeneration system，cotyledonry nodes organ of new soybean line Heinong 56 was used as explants.The effect of different concentration of 6-BA and IBA on the ratio of shooting was investigated in the shoot induction medium.The optimum concentration of 6-BA and IBA was 2.0 mgL-1，0.2 mgL-1,respectively.Adopting the model of late selection，the optimal concentration of Glufosinate-ammonium was 4.0 mgL-1in phase of shoots induction.

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备注/Memo

基金项目：国家高技术研究发展计划（863计划）资助项目（2006AA10ZF9）；国家转基因重大专项（2008ZX08004-002）资助项目。

作者简介：寇坤(198-)，女，硕士研究生，研究方向为分子育种。E-mail:koukun2006@163.com。
通讯作者：刘丽君，研究员。E-mail:nkyssbd@126.com。

更新日期/Last Update: 2014-09-25