[1]陈相艳,李伟,戴海英,等.大豆EST资源的SSR信息分析[J].大豆科学,2009,28(03):394-399.[doi:10.11861/j.issn.1000-9841.2009.03.0394]
　CHEN Xiang-yan,LI Wei,DAI Hai-ying,et al.Analysis of SSR Information in EST Resource of Soybean（Glycine max)[J].Soybean Science,2009,28(03):394-399.[doi:10.11861/j.issn.1000-9841.2009.03.0394]
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大豆EST资源的SSR信息分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年03期 页码: 394-399 栏目: 出版日期: 2009-06-25
Title:
Analysis of SSR Information in EST Resource of Soybean（Glycine max)
文章编号:
1000-9841(2009)03-0394-06
作者:
陈相艳1; 李伟2; 戴海英2; 张礼凤2
1山东省农业科学院办公室，山东 济南 250100；
2山东省农业科学院作物所，山东 济南 250100
Author(s):
CHEN Xiang-yan1; LI Wei2; DAI Hai-ying2; ZHANG Li-feng2
1Office，Shandong Academy of Agricultural Sciences，Jinan 250100，Shangdong；
2Crop Institute，Shandong Academy of Agricultural Sciences，Jinan 250100，Shandong，China
关键词:
大豆; EST; SSR; 频率; 特性
Keywords:
Soybean; EST; SSR; Frequency; Characteristics
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.03.0394
文献标志码:
A
摘要:
微卫星或简单重复序列（simple sequence repeats，SSR）存在于表达序列标签（expressed sequence tags，ESTs）中。为了在大豆中开发EST-SSR功能性标记，利用生物信息学对NCBI公共数据库中的394 370条大豆ESTs序列进行EST-SSRs特征分析。剔除冗余序列，得到全长为51 332.21 kb的无冗余EST 80 735条。在这些序列中搜索出7 754个SSR，分布于6 674条EST中，出现频率是8.27%。这些EST-SSR的平均长度为15.26 bp，平均分布频率是1/6.62 kb。在1~6 bp的重复基元中，三核苷酸重复基元的SSRs出现频率最高（43.72%），其次是二核苷酸（37.34%）、单核苷酸（15.92%）。AG/CT和AAG/CTT是二、三核苷酸中的优势重复基元，分别占二、三核苷酸重复的62.83%和25.22%。本研究为开发多态性大豆微卫星标记提供了候选序列。
Abstract:
Expressed sequence tags（ESTs）are important resources for development of new SSR markers.In this study，394 370 ESTs of soybean（Glycine max L.）in the database of NCBI were downloaded and analyzed.After the preprocession，resulting in 80 735 non-redundant ESTs with total length about 51 332.21 kb.Totally 7 754 SSRs distributed in 6 674 ESTs were detected，accounting for 8.27% of the non-redundant ESTs.The average length and distribution distance of the EST-SSRs were about 15.26 bp and 6.62 kb，respectively.Dinucleotide and trinucleotide repeats with similar frequency are the main types，accounting for 81.06% of all the SSRs.AG/CT and AAG/CTT are the most frequent motifs，accounting for 62.83% and 25.22% in the dinucleotide and trinucleotide repeats，respectively.These EST-SSRs will help to develop new SSR markers with high polymorphism for soybean.

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备注/Memo

基金项目：山东省自然科学基金资助项目（Y2006D20）。

作者简介：陈相艳（1973-）女，硕士，现主要从事大豆育种工作；李伟为共同第一作者。
通讯作者：张礼凤，副研究员。E-mail：zhanglifeng9639@sina.com。

更新日期/Last Update: 2014-09-25