[1]赫卫,姜振峰,赵琳,等.不同光长条件下大豆蛋白质组比较研究[J].大豆科学,2009,28(03):388-393.[doi:10.11861/j.issn.1000-9841.2009.03.0388]
　HE Wei,JIANG Zhen-feng,ZHAO Lin,et al.A Comparative Study on Soybean Leaf Proteomics under Different Photoperiod Treatments[J].Soybean Science,2009,28(03):388-393.[doi:10.11861/j.issn.1000-9841.2009.03.0388]
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不同光长条件下大豆蛋白质组比较研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年03期 页码: 388-393 栏目: 出版日期: 2009-06-25
Title:
A Comparative Study on Soybean Leaf Proteomics under Different Photoperiod Treatments
文章编号:
1000-9841(2009)03-0388-06
作者:
赫卫; 姜振峰; 赵琳; 韩英鹏; 李文滨
（东北农业大学大豆研究所，教育部大豆生物学重点实验室，黑龙江 哈尔滨 150030）
Author(s):
HE Wei; JIANG Zhen-feng; ZHAO Lin; HAN Ying-peng; LI Wen-bin
Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology of Chinese Education Ministry，Harbin 150030，Heilongjiang，China
关键词:
大豆; 双向电泳(2-DE); 蛋白质组学; 光周期; 离子阱质谱分析
Keywords:
Soybean; Two-dimensional gel electrophoresis（2-DE); Proteomics; Photoperiod; Liquid and ion trap mass spectrometry
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.03.0388
文献标志码:
A
摘要:
对大豆光周期敏感型品种东农42分别进行16 h光照和8 h光照处理，提取处理和对照相同叶位叶片的全蛋白质进行双向电泳，通过PDQuest和液相离子阱质谱(LC-ESI-MS)技术对差异表达蛋白进行分析，获得二级质谱图谱，用Mascot软件搜索NCBInr数据库，鉴定出16个差异表达蛋白，其中12个表达量上调，4个表达量下调。对谷胱甘肽 S转移酶、二硫键异构酶、TCTP、半胱氨酸合成酶、Rieske铁硫蛋白前体、金属蛋白酶等主要差异蛋白进行功能分析，表明这些蛋白与植物的光合作用、信号转导、细胞凋亡的调控、防卫解毒和能量代谢等多种植物的生理生化功能相关。
Abstract:
Dongnong 42，a photoperiod sensitive cultivar，was treated with 8 h and 16 h light illuminations，respectively.The leaf tissues were then sampled and two-dimensional gel electrophoresis（2-DE）was performed to separate the protein spots.The gels were stained with Coomassie Blue G-250.The difference between the protein maps of leaves from two different photoperiod treatments was analyzed with the PDQuest image software(Bio-rad).The liquid and ion trap mass spectrometry（LC-ESI-MS）was used to obtain the MS/MS of the differentially expressed proteins and the MASCOT software was used to search the protein database.A total of 16 differentially expressed proteins were identified，of them nine proteins showed up-regulation expression and four showed down-regulation expression.The major differentially expressed proteins，glutathione-S-transferase，disulfide isomerase，metalloproteinases，TCTP，Rieske iron-sulphur protein precursor cysteine synthetic enzymes，were reviewed and discussed.These proteins might be related to plant photosynthesis，signal transduction and apoptosis control，detoxification and plant defense and other physiological and biochemical function.

参考文献/References:

[1] Garner W W，Allard H A.Effect of the relative length of day and night and other factors of the environment on growth and reproduction in plants[J].Journal of Agricultural Research，1920，18：553-606.

[2] Mooney B P，Thelen J J.High-through put peptide mass finger printing of soybean seed proteins ：automated workflow and utility of UniGene expressed sequence tag databases for protein identification[J].Phytochemistry，2004，65:1733-1744.
[3] Hajduch M，Ganapathy A，Stein J W.A Systematic proteomic study of seed filling in soybean.Establishment of high resolution two-dimensional reference maps，expression profiles，and an interactive proteome database[J].Plant Physiology，2005，137 ：1397-1419.
[4]徐晓燕，郑蕊，立春梅，等.大豆种子萌发过程中的差异蛋质组研究[J].生物化学与生物物理进展，2006，33(11)：1106-1112.（Xu X Y，Zheng R，Li C M，et al.Differential proteomic analysis of seed germination in soybean[J].Progress in Biochemistry and Biophysics，2006，33(11):1106-1112.）
[5]郑蕊，喻德跃.适于蛋白质组研究的大豆种子双向电泳技术的改进[J].大豆科学，2005，24（3）:166-170.(Zheng R，Yu D Y.Improvement of two-dimensional electrophoresis of bean seed protein for proteome analysis[J].Soybean Science，2005 ，24（3）:166-170.)
?[6]曾维英，杨守萍，喻德跃，等.大豆质核互作雄性不育系NJCMS2A及其保持系的花药蛋质组比较研究［J］.作物学报，2007，33(10):1637-1643.（Zeng W Y，Yang S P，Yu D Y.A comparative study on anther proteomics between cytoplasmic-nuclear male-sterile line NJCMS2A and its maintainer of soybean[J].Acta Agronomica Sinica，2007，33(10):1637-1643.）
[7]陈丽霞.利用双向电泳技术分离大豆矮秆突变体相关蛋白 [J].中国生物工程杂志，2007，27(3)：76-82.(Chen L x，LiY H，Ren Z Y.Isolation of proteins related to the dwarf mutant by two-dimensional gel electrophoresis in soybean[J].China Biotechnology，2007，27(3)：76-82.)
[8] Bennett J O，Yu O，Heatherly LG，et al.Accumulation of genistein and daidzein，soybean isoflavones implicated in promoting human health，is significantly elevated by irrigation[J].Journal of Agricultural and Food Chemistry，2004，52(25):7574-7579.
[9] Krishnan H B，Bennett J O，Kim W S，et al.Nitrogen lowers the sulfur amino acid content of soybean(Glycine max [L.] Merr.)by regulating the accumulation of Bowman-Birk protease inhibitor[J].Journal of Agricultural and Food Chemistry，2005，53(16):6347-6354.
[10] Natarajan S S，Xu C，Bae H，et al.Characterization of storage proteins in wild（Glycine soja)and cultivated(Glycine max)soybean seeds using proteomic analysis.[J].Journal of Agricultural and Food Chemistry，2006，54(8):3114-3120.
[11] Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding[J].Analytical Biochemistry，1976 ，72 ：248-254.
[12] Candiano G，Bruschi M，Musante L，et al.Blue silver：A very sensitive colloidal coomassie G-250 staining for proteoe analysis[J].Electrophoresis，2004，25:1327-1333.
[13]何大澄，肖雪媛.差异蛋白质组学及其应用 [J].北京师范大学学报.2002，38(4):558-562.（He D C，Xiao X Y.Differential proteomics and its applications [J].Journal of Beijing Normal University Science，2002，38(4):558-562.）
[14] Chen I C，Huang I C，Liu M J，et al.Glutathione S-transferase interacting with far-red insensitive 219 is involved in phytochrome a-mediated signaling in arabidopsis [J].Plant Physiology，2007,143(3)：1189-1202.
[15] Gong H B，Jiao Y X，Hui W W,et al.Expression of glutathione-S-transferase and its role in plant growth and development in vivo and shoot morphogenesis in vitro [J].Plant Molecular Biology，2005，57:53-66.
[16] Gerard M.Sequencing and characterization of the soybean leaf metalloproteinase：structural and functional similarity to the matrix metalloproteinase family [J].Plant Physiology，1992，99，1179-1183.
[17] Dortje G，Olga V P，Karl J D.Mutation of the matrix metalloproteinase At2-MMP inhibits growth and causes late flowering and early senescence in arabidopsis [J].Biological Chemistry，2002，277（7）:5541-5547.
[18] Sage-Ono K, Ono M, Harada H, Kamada H.Dark-induced accumulation of mRNA for a homolog of translationally controlled tumor protein（TCTP）in Pharbitis [J]. Plant & Cell Physiology,1998，39(3):357-60.
[19] Bommer U A，Anton V，Greagg M A，et al.The mRNA of the translationally controlled tumor protein P23/TCTP is a highly structured RNA，which activates the dsRNA-dependent protein kinase[J].RNA（New York，N.Y.)，2002，8:478-496.
[20] Michael R S.The Light Reactions：A guide to recent acquisitions for the picture gallery[J].The Plant Cell，2005，17(3)：648-663.
[21]刘琼明，张令强，贺福初，等.氧化还原信号转导的分子机制[J].生命的化学，2005，25（1):1-3.(Liu Q M，Zhang L Q，He F C.Molecular mechanisms of redox signal transduction[J].Chemistry of Life，2005，25（1):1-3.）
[22] Bowler C，Alliotte T，De Loose M，et al.The induction of manganese superoxide dismutase in response to stress in nicotiana plumbaginifolia[J].The European Molecular Biology Organization Journal,1989，8(1):31-38.

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备注/Memo

基金项目：国家自然科学基金资助项目（30671318）；第四十三批中国博士后科学基金面上资助项目；东北农业大学博士启动基金资助项目。

作者简介：赫卫（1981-），女，硕士研究生，研究方向为大豆蛋白质组学研究。E-mail: hw.he@yahoo.com.cn。
通讯作者：李文滨，教授，博士生导师。E-mail:wenbinli@yahoo.com。

更新日期/Last Update: 2014-09-25