[1]牛丽颖,刘敏彦,王玉峰,等.河北产淡豆豉黄酮类成分HPLC指纹图谱研究[J].大豆科学,2009,28(02):329-331.[doi:10.11861/j.issn.1000-9841.2009.02.0329]
　NIU Li-ying,LIU Min-yan,WANG Yu-feng,et al.HPLC Fingerprint Analysis of Flavonoids in Semen Sojae Praeparatum in Hebei Province[J].Soybean Science,2009,28(02):329-331.[doi:10.11861/j.issn.1000-9841.2009.02.0329]
点击复制

河北产淡豆豉黄酮类成分HPLC指纹图谱研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 329-331 栏目: 出版日期: 2009-04-25
Title:
HPLC Fingerprint Analysis of Flavonoids in Semen Sojae Praeparatum in Hebei Province
文章编号:
1000-9841(2009)020329-03
作者:
牛丽颖1; 刘敏彦2; 王玉峰2; 杜红娜2; 王鑫国1
1河北医科大学中医学院，河北 石家庄 050091；
2河北医科大学医药研究院，河北 石家庄 050035
Author(s):
NIU Li-ying1; LIU Min-yan2; WANG Yu-feng2; DU Hong-na2; WANG Xin-guo1
1College of Traditional Chinese Medicine，Hebei Medical University，Shijiazhuang 050091，Hebei；
2Academy of Medicine，Hebei Medical University，Shijiazhuang 050035，Hebei，China
关键词:
淡豆豉; 黄酮类成分; HPLC; 指纹图谱
Keywords:
Semen Sojae Praeparatum; Flavonoids; HPLC; Fingerprint
分类号:
R25
DOI:
10.11861/j.issn.1000-9841.2009.02.0329
文献标志码:
A
摘要:
建立河北产淡豆豉黄酮类成分HPLC指纹图谱，控制淡豆豉的质量。采用3%冰乙酸-乙腈，梯度洗脱的方法进行色谱分离，对各色谱峰进行数据处理，得出淡豆豉的指纹图谱。标定了淡豆豉13个共有峰，且共有峰重现性良好。采用HPLC方法制定的淡豆豉的指纹图谱，可用于淡豆豉的质量评价。
Abstract:
To establish fingerprint chromatographic of flavonoids components in Semen Sojae Praeparatum in Hebei Province by HPLC and control its quality.Gradient elution of 3%acetic acid  acetonitril was applied to chromatographic peaks separation and data were analyzed to develop HPLC fingerprint analysis of Semen Sojae Praeparatum.Thirteen peaks were demarcated in Semen Sojae Praeparatum and they were all reapeatable.The HPLC fingerprint can be used to evaluate the quality of Semen Sojae Praeparatum.

参考文献/References:

[1]国家药典委员会编.中华人民共和国药典(一部)[S].北京:化学工业出版社，2005.(Chinese Pharmacopoeia Committee.China pharmacopoeia version(part one)[S].Beijing：Chemical Industrial Press，2005.)

[2]王鑫国，葛喜珍，白霞，等.淡豆豉对去卵巢大鼠脂代谢的影响[J].中药材，2003，26(9):652-654.(Wang X G，Ge X Z，Bai X，et al.Effects of Semen Sojae Preparatum on lipid metabolism in ovariectomized Rat[J].Journal of Chinese Medicinal Materials，2003，26(9)：652-654.）
[3]高淑丽，牛丽颍，曹秀莲，等.淡豆豉提取物抗心肌缺血作用的研究[J].河北医药，2007，29(9):923-924.(Gao S L，Niu L Y，Cao X L，et al，Protective effect of fermented soybean extraction on myocardial ischemia in mice[J].Hebei Medical Journal，2007，29(9):923-924.)
[4]牛丽颖，刘娇，崔力剑，等.淡豆豉对早期动脉粥样硬化大鼠血管内皮损伤的保护作用[J].中药药理与临床，2007，23(5):923-924.(Niu L Y，Liu J，Cui L J，et al.Effects and mechanisms of Semen Sojae Preparatum extracts on rat’s injury at the early stage of atherosclerosis[J].Pharmacology and Clinics of Chinese Materia Medica，2007，23(5):923-924.)
[5]牛丽颖，王鑫国，葛喜珍，等.淡豆豉提取物降糖有效部位研究[J].中药药理与临床，2004；23(5):21-22.(Niu L Y，Wang X G，Ge X Z，et al.Study on hypoglycemic effective part of Semen Sojae Preparatum extracts[J].Pharmacology and Clinics of Chinese Materia Medica，2004；23(5):21-22.)
[6]白霞，牛丽颖，刘娇，等.淡豆豉防治早期动脉粥样硬化大鼠血管损伤的机制研究[J].时珍国医国药，2008，19(1):170-171.( Bai X，Niu L Y，Liu J，et al.Mechanisms of Semen Sojae Preparatum extracts on rat’s injury at the early stage of atherosclerosis[J].Lishizhen Medicine and Materia Medica Research，2008，19(1):170-171.)
[7]曹秀莲，牛丽颍，窦玉红，等.淡豆豉对心肌缺血小鼠心肌一氧化氮合酶表达的影响[J].河北中医药学报，2007，22(4):3-4.(Cao X L，Niu L Y，Dou L H，et al.Effects of Semen Sojae Preparatum on the NOS expression of myocardial ischemia in mice[J].Hebei Medical Journals，2007，22(4):3-4.)
[8]王鑫国，郭秋红，白霞，等.乌鸡白凤丸对去卵巢大鼠雌激素分泌的影响[J].中成药，2003，25（1）:67-68.( Wang X G，Guo Q H，Bai X.The effect of white phoenix bolus of black bone chicken on oestrogen level in ovariectomized Ra[J].Journal of Chinese Medicinal Materials，2003，25(1):67-68.)
[9]张娟，卿德刚，倪慧，等.红花黄色素HPLC指纹图谱研究[J].食品科学，2008，29(1):257-259.(Zhang J，Qing D G，Ni H，et al.Study on HPLC fingerprint analysis of safflor yellow.[J].Food Science，2008，29(1):257-259.)
[10]牛丽颖，杜红娜，刘姣，等.淡豆豉炮制前后异黄酮组分含量的比较研究[J].大豆科学，2008，27（4）：672-674，678.( Niu L Y，Du H N，Liu J，et al.The comparative study on the content of isoflavone in Sojae Preparatum extracts before and after processing[J].Soybean Science，2008，27（4）：672-674，678.)

相似文献/References:

[1]崔力剑,石素琴,刘敏彦,等.淡豆豉中多指标成分的含量测定方法研究[J].大豆科学,2012,31(01):127.[doi:10.3969/j.issn.1000-9841.2012.01.029]
　CUI li-jian,SHI Su-qin,LIU Min-yan,et al.Determination Method of Multi-target Components in Semen Sojae Praeparatum[J].Soybean Science,2012,31(02):127.[doi:10.3969/j.issn.1000-9841.2012.01.029]
[2]张德花,王淑敏,谢兆莉,等.淡豆豉炮制工艺中加入人参对大豆异黄酮含量的影响[J].大豆科学,2012,31(02):288.[doi:10.3969/j.issn.1000-9841.2012.02.027]
　ZHANG De-hua,WANG Shu-min,XIE Zhao-li,et al.Influence of Adding Ginseng to Semen Sojae Preparatum on Content of Soybean Isoflavones[J].Soybean Science,2012,31(02):288.[doi:10.3969/j.issn.1000-9841.2012.02.027]
[3]牛丽颖,蔡广华,刘敏彦,等.淡豆豉中异黄酮提取工艺的优化研究[J].大豆科学,2011,30(01):164.[doi:10.11861/j.issn.1000-9841.2011.01.0164]
　NIU Li-ying,CAI Guang-hua,LIU Min-yan,et al.Research on Optimization of Isoflavone Extracting Technique from Semen Sojae Praeparatum[J].Soybean Science,2011,30(02):164.[doi:10.11861/j.issn.1000-9841.2011.01.0164]
[4]牛丽颖,蔡广华,王鑫国.利用ADS-7大孔树脂分离纯化淡豆豉中异黄酮[J].大豆科学,2010,29(01):164.[doi:10.11861/j.issn.1000-9841.2010.01.0164]
　NIU Li-ying,CAI Guang-hua,WANG Xin-guo.Separation and Purification of Soybean Isoflavone in Semen Sojae Praeparatum by ADS-7 Macroporous Adsorptive Resins[J].Soybean Science,2010,29(02):164.[doi:10.11861/j.issn.1000-9841.2010.01.0164]
[5]牛丽颖,杜红娜,刘姣,等.淡豆豉炮制前后异黄酮组分含量的比较[J].大豆科学,2008,27(04):672.[doi:10.11861/j.issn.1000-9841.2008.04.0672]
　NIU Li-ying,DU Hong-na,LIU Jiao,et al.Comparative Analysis of Isoflavone Contents in Semen Sojae Praeparatum Before and After Processing[J].Soybean Science,2008,27(02):672.[doi:10.11861/j.issn.1000-9841.2008.04.0672]
[6]崔力剑,黄芸,詹文红,等.发酵处理对大豆中总异黄酮含量的影响[J].大豆科学,2007,26(04):588.[doi:10.3969/j.issn.1000-9841.2007.04.026]
　CUI Li-jian,HUANG Yun,ZHAN Wen-hong,et al.EFFECT OF FERMENTATION PROCESS ON TOTAL CONTENT OF ISOFLAVONES IN SOYBEAN[J].Soybean Science,2007,26(02):588.[doi:10.3969/j.issn.1000-9841.2007.04.026]
[7]李东文,朱海针,苏明声,等.Biolog-ECO解析淡豆豉发酵过程中微生物群落碳代谢特征[J].大豆科学,2015,34(05):889.[doi:10.11861/j.issn.1000-9841.2015.05.0889]
　LI Dong-wen,ZHU Hai-zhen,SU Ming-sheng,et al.An Analysis of Carbon Metabolic Characteristics of Microbial Community in the Fermentation Process of Fermented Soybean Using Biolog-ECO Method[J].Soybean Science,2015,34(02):889.[doi:10.11861/j.issn.1000-9841.2015.05.0889]
[8]支雅婧,王梦,甄亚钦,等.淡豆豉活性成分一测多评检测方法建立[J].大豆科学,2021,40(05):696.[doi:10.11861/j.issn.1000-9841.2021.05.0696]
　ZHI Ya-jing,WANG Meng,ZHEN Ya-qin,et al.Determination of Active Ingredients of Sojae Semen Praeparatum by Multi-Components with Single Marker[J].Soybean Science,2021,40(02):696.[doi:10.11861/j.issn.1000-9841.2021.05.0696]
[9]李尚逊,姬翔,翟兴媛,等.异常威克汉姆酵母纯种发酵淡豆豉的炮制工艺研究[J].大豆科学,2023,42(04):459.[doi:10.11861/j.issn.1000-9841.2023.04.0459]

备注/Memo

作者简介：牛丽颖（1968-），女，教授，硕士生导师，现主要从事中药物质基础研究工作。

通讯作者：王鑫国，教授。E-mail:wangxinguozy@163.com

更新日期/Last Update: 2014-10-04