[1]王丹,王丕武,付永平,等.大豆根部特异性启动子的克隆及功能分析[J].大豆科学,2009,28(02):195-199.[doi:10.11861/j.issn.1000-9841.2009.02.0195]
　WANG Dan,WANG Pi-wu,FU Yong-ping,et al.Cloning and Identification of the Root Specific Promoter from Soybean[J].Soybean Science,2009,28(02):195-199.[doi:10.11861/j.issn.1000-9841.2009.02.0195]
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大豆根部特异性启动子的克隆及功能分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年02期 页码: 195-199 栏目: 出版日期: 2009-04-25
Title:
Cloning and Identification of the Root Specific Promoter from Soybean
文章编号:
1000-9841(2009)02-0195-05
作者:
王丹; 王丕武; 付永平; 厉志
吉林农业大学农学院，吉林 长春 130118
Author(s):
WANG Dan; WANG Pi-wu; FU Yong-ping; LI Zhi
College of Agriculture，Jilin Agricultural University，Changchun 130118,Jilin，China
关键词:
大豆; 根部特异性启动子; 功能分析; GUS染色
Keywords:
Soybean; Root specific promoter; Functional Identification; GUS staining
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0195
文献标志码:
A
摘要:
利用 PCR技术从大豆品种吉农17基因组 DNA中分离得到大豆根部特异性启动子片段RSP，长度约为2.5 kb。序列分析表明RSP与报道序列同源性达97％以上。将其与GUS基因融合，构建植物表达载体pRSP-GUS后，转化到EHA105根癌农杆菌中，通过根癌农杆菌(Agrobacterium tumefaciens)介导法转化烟草(Nicotiana tabacum)NC89。转基因植株的GUS活性检测表明，仅能在根中检测到GUS活性，而在茎、叶和种子等其它组织中都未检测到GUS活性，证实RSP片段具有根部特异性表达功能。
Abstract:
The promoter region of root-specific gene was isolated from the genomic DNA of soybean Jilin 17 by PCR method，and obtained the promoter fragment RSP，the length of the clone sequence is 2.5kb．The results of sequence analysis indicated that this fragment had 97％ homology compared with the reported promoters．The cloned promoter was fused to the GUS reporter gene to construct plant expression vector pRSP-GUS，which was transferred into tobacco(Nicotiana tabacum)NC89 by Agrobacterium tumefaciens-mediated method and severa1 transformed plants were obtained.GUS activity assays of different soybean organs indicated that expression of GUS was active only in roots，which suggests the RSP gene is root-specific promoter.

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备注/Memo

基金项目：教育部博士点基金资助项目(20070193005)。

作者简介：王丹(1983-)，女，硕士研究生，研究方向为生物技术在作物遗传育种中的应用。

更新日期/Last Update: 2014-10-03