[1]赵宇枢,段玉玺,王媛媛,等.辽宁省大豆根瘤菌资源抗逆性及生防潜力研究[J].大豆科学,2009,28(01):113-117.[doi:10.11861/j.issn.1000-9841.2009.01.0113]
　ZHAO Yu-shu,DUAN Yu-xi,WANG Yuan-yuan,et al.Stress Resistance and Biocontrol Potential of Soybean Rhizobia Resourses Isolated From Liaoning Province[J].Soybean Science,2009,28(01):113-117.[doi:10.11861/j.issn.1000-9841.2009.01.0113]
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辽宁省大豆根瘤菌资源抗逆性及生防潜力研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第28卷 期数: 2009年01期 页码: 113-117 栏目: 出版日期: 2009-02-25
Title:
Stress Resistance and Biocontrol Potential of Soybean Rhizobia Resourses Isolated From Liaoning Province
文章编号:
1000-9841(2009)01-0113-05
作者:
赵宇枢1; 段玉玺1; 王媛媛2; 陈立杰1; 尹丽娜1
1沈阳农业大学植物保护学院，辽宁 沈阳 110161；
2沈阳农业大学生物科学技术学院，辽宁 沈阳 110161
Author(s):
ZHAO Yu-shu1; DUAN Yu-xi 1; WANG Yuan-yuan2; CHEN Li-jie1; YIN Li-na1
1Department of Plant Protection，Shenyang Agricultural University，Shenyang 110161，Liaoning；
2Department of Biological Science and Technology，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
关键词:
大豆; 根瘤菌; 抗逆性; 生防潜力
Keywords:
Soybean; Rhizobia; Stress tolerance; Biocontrol potential
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0113
文献标志码:
A
摘要:
从辽宁省不同地理分布区采集土样并诱集根瘤；采用组织分离法分离获得16株根瘤菌。测定其对酸、碱、盐、氨、温度的耐受性和对大豆根部病原微生物的抑制作用。结果表明：分离得到的16株根瘤菌的抗逆性及拮抗活性在不同菌株之间表现出明显差异。其中，菌株Snb119、Snb126、Snb183对酸、碱、盐、氨、高温、低温，有较强的耐受性，菌株Snb183、Snb204、Snb389、Snb394可有效抑制腐霉菌，菌株Snb183，Snb241对大豆胞囊线虫二龄幼虫具有抑制作用。
Abstract:
In order to make clear the endurance abilities and the biocontrol potential of soybean rhizobia resourse of Liaoning province.The growth or survival abilities of soybean rhizobia strains isolated from different area were tested under the stresses of acid，alkali，NaCl，(NH-4)2SO4，high temperature，low temperature，and their potential to control soybean root pathogens was researched.Soil samples were collected from different geographical area in Liaoning province and soybean root nodules were trapped from the soil sample；16 strains of soybean rhizobia were isolated by tissue isolation.The results showed that the stress-tolerant capability and antagonistic effect of the 16 rhizobia strains differ greatly.Stains Snb119，Snb126，Snb183 can grow under the stresses of acid，alkali，NaCl，(NH4)2SO4，high temperature，low temperature.Snb183，strains Snb204，Snb389，Snb394 inhibit growth of Pythium.spp，Snb183，Snb241 strains suppress J2 of soybean cyst nematode.

参考文献/References:

[1]赵团结，盖钧镒，李海旺，等.超高产大豆育种研究的进展与讨论[J].中国农业科学，2006，39（1）：29-37.(Zhao T J，Gai J Y，Li H W，et al.Advances in breeding for super high yielding soybean cultivars[J].Scientia Agricultura Sinica，2006，39（1）：29-37.)

[2]蒋慕东，盖钧镒.中国大豆应自主沉浮[J].中国食物与营养，2006，8：4-7.(Jiang M D，Gai J Y.The Chinese soybean shoud sink independently floats[J].Food and Nutrition in China，2006，8：4-7.)
[3]陈文新，陈文峰.发挥生物固氮作用减少化学氮肥用量[J].中国农业科技导报，2004，6（6）：3-6.(Chen W X，Chen W F.Exertion of biological nitrogen fixation in order to reducing the consumption of chemical nitrogenous fertilizer[J].Review of China Agriculture Science and Technology，2004，6（6）：3-6.)
[4]朱剑光，尉亚辉，吴艺舟.花生慢生根瘤菌的分离与鉴定[J].生物技术，2006，16（2）：45-47.(Zhu J G，Wei Y H，Wu Y Z. Isolation and identify of bradyrhizobium bacterium from peanut[J].Biotechnology，2006，16（2）：45-47.)
[5]东秀珠，蔡妙英.常见细菌系统鉴定手册[M].北京：科学出版社，2001：364-369.(Dong X Z，Cai M Y.Manual of determinative systematic bacteriology[M].Beijing：Science Press，2001：364-369.
[6]徐传瑞.高效固氮大豆根瘤菌的筛选和鉴定[D].武汉：华中农业大学，2004：21-47.(Xu C R.Screening and identification of highly efficient Rhizobia[D].Wuhan：Huazhong Agricultural University，2004：21-47.)
[7]王媛媛，段玉玺，陈立杰.根瘤内生细菌对大豆胞囊线虫及根腐病菌的影响[J].大豆科学，2007，29（2）：213-217.(Wang Y Y，Duan Y X，Cheng L J.Effect of endophytic bacteria from soybean root nodules on soybean cyst nematode and pathogens of soybean root rot[J].Soybean Science，2007，29（2）：213-217.)
[8]刘维志.植物线虫学研究技术[M].沈阳：辽宁科学技术出版社，1995.36-41.(Liu W Z.The technique of plant nematode research[M].Shenyang：Liaoning Science and Technology Press，1995：36-41.)
[9]陈文新，汪恩涛，陈文峰.根瘤菌豆科植物共生多样性与地理环境的关系[J].中国农业科学，2004，37（1）：81-86.(Chen W X，Wang E T，Chen W F.The relationship between the symbiotic promiscuity of rhizobia and legumes and their geographical environments[J].Scientia Agricultura Sinica，2004，37（1）：81-86.
[10] Bardin S D，Hang H C，Pinto J，et al.Biological control of Pythium damping off of pea and augar beet by Rhizobium Leguminosarum bv.viceae[J].Canadian Journal of Botany，2004，82(3)：291-296.
[11] Siddiqui I A，Amer Zareen S，Zaki M J，et al.Evaluation of Rhizobia for the control of meloidogyne javanica in Vigna mungo[J].Pakistan Journal of Biological Scientific Information，2001，4(9)：1124-1125.
[12] Shaukat S S，Siddiqui I A，Hamid M，et al.In vitro survival and nematicidal acticity of rhizobium，bradyrhizobium and sinorhizobium.l.The Influence of various NaCl concentrations[J].Pakistan Journal of Biological Sciences，2002，5(6)：669-671.
[13]李力，曹凤明，徐玲玫，等.花生根瘤菌的抗逆性初步研究[J].微生物学通报，2000，27（1）：42-47.(Li L，Cao F M，Xu M L，et al.Preliminary study on peanut rhizobia of China for tolerance to stresses[J].Microbiology，2000，27（1）：42-47.

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备注/Memo

基金项目：十一五国家科技支撑计划资助项目(2006BAD08A08)；农业部科技成果转化基金项目（05EFN212100059）；国家自然科学基金资助项目（30673199）。

作者简介：赵宇枢（1983-），女，硕士研究生，现从事生物多样性与生物防治方面研究。E-mail:zhaoyushu232@163.com。

更新日期/Last Update: 2014-10-03