[1]胡国玉,赵晋铭,周斌,等.大豆耐低温出苗的遗传分析与分子标记[J].大豆科学,2008,27(06):905-910.[doi:10.11861/j.issn.1000-9841.2008.06.0905]
　HU Guo-yu,ZHAO Jin-ming,ZHOU Bin,et al.Inheritance and Molecular Marker of Chilling Tolerance of Soybean in EarlyStage[J].Soybean Science,2008,27(06):905-910.[doi:10.11861/j.issn.1000-9841.2008.06.0905]
点击复制

大豆耐低温出苗的遗传分析与分子标记

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年06期 页码: 905-910 栏目: 出版日期: 2008-12-25
Title:
Inheritance and Molecular Marker of Chilling Tolerance of Soybean in EarlyStage
文章编号:
1000-9841(2008)06-0905-06
作者:
胡国玉; 赵晋铭; 周斌; 左巧美; 盖钧镒; 喻德跃; 邢邯
南京农业大学大豆研究所，国家大豆改良中心，作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
HU Guo-yu; ZHAO Jin-ming; ZHOU Bin; ZUO Qiao-mei; GAI Jun-yi; YU De-yue; XING Han
Soybean Research Institute，National Center for Soybean Improvement，Ministry of Agriculture,National Key Laboratory of Crop Genetics and Germplasm Enhancement，Nanjng Agricultural University,Nanjing 210095，Jiangsu,China
关键词:
大豆; 生长初期; 耐低温; 遗传分析; 分子标记
Keywords:
Soybean; Early stage; Chilling tolerance; Inheritance; Molecular marker
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.0905
文献标志码:
A
摘要:
大豆是光温敏性作物，其对低温的敏感性不但制约了大豆在高纬度地区的生产，也严重影响低纬度地区低温年大豆的种植产量。以周9311-3×徐9125的P1、P2、F1、F2、F2：3为材料用主基因-多基因混合遗传模型分离分析法，对大豆耐低温出苗性状进行遗传分析，然后采用BSA和t测验的方法分析筛选与大豆耐低温出苗性状紧密连锁的SSR分子标记。结果表明：大豆耐低温出苗性状是受一对主基因控制，其遗传模型的适合性检验表明符合D-0模型，即耐低温性状受一对加性-显性主基因+加性-显性-上位性多基因控制，主基因呈超显性。大豆耐低温出苗性状和标记satt157及satt562相关，并根据R2得到两位点的变异解释率分别为3.4%和13.9%。根据已经发表的整合大豆遗传图谱中satt157、satt562的位置将大豆耐低温出苗的两个QTL初步定位于D1b和I连锁群。satt562的效应值较大属于主基因位点，satt157的效应值较小属于多基因位点。
Abstract:
Soybean is an important source of human protein and oil，and it is susceptible to temperature and light.Low temperature susceptibility affects soybean yield not only in high latitude area but also in low.Inheritance of soybean chilling tolerance in early stage was analyzed jointly in P1and P2parents，F1，F2and F2:3generations derived from the cross Zhou 9311-3×Xu 9125 by using the joint segregation analysis of the mixed major gene and polygene inheritance model of quantitative traits.And then through t-test analysis and bulked segregate analysis(BSA)，SSR markers which linked lightly with chilling tolerance were screened.The results indicated that the inheritance of chilling tolerance in the population was accorded with the D-0 model，namely the inheritance of the chilling tolerance in the population was controlled by one major gene with additive-dominance effect plus polygene with additive-dominance-epistatic effect，and the major gene showed overdominance effect.Two molecular markers satt562 and satt157were found linked with the chilling tolerance genes in the F2 population of Zhou 9311-3×Xu 9125.The individual effect of each marker was reported as R-2value，and explained 13.9% and 3.4% respectively.And the markers of satt562 and satt157 situated on I and D1b linkage groups，respectively.The satt562 with larger phenotypic effect could be regard as major gene locus，and satt157 with relative smaller effects could be regarded as polygene locus.

参考文献/References:

[1]张德荣，张学君，孟祥勋.大豆低温冷害敏感时期试验研究报告[J].吉林农业科学，1987（1）：37-38.（Zhang D R，Zhang X J，Meng X X.Study report on chilling sensitive stage of soybean[J].Jilin Agricultural Science，1987(1)：37-38.）

[2]潘铁夫.东北地区农作物冷害发生规律的研究[J].吉林农业科学，1985（4）：9-16.（Pan T F.Law of chilling stress in North-east area[J].Jilin Agricultural Science，1985(4)：9-16.）
[3]王金陵.中国东北大豆[M].哈尔滨：黑龙江科学技术出版社，1999：11-20.（Wang J L.Soybean of North-east area in China [M].Harbin：Heilongjiang Science & Tech Press，1999：11-20.）
[4]杨加银.菜用大豆育种目标与策略的商榷[J].种子，2004，23（12）：46-47.（Yang J Y.Discussion on objective and strategy of vegetable soybean breeding[J].Seed，2004，23(12)：46-47.）
[5]郭泰，齐宁，刘忠堂，等.大豆品种资源及F 2和F3种子萌发期耐冷性鉴定[J].黑龙江农业科学，1995，1：6-8.（Guo T，Qi N，Liu Z T，et al.The identification on tolerance to cold in the germination of soybean gerrmplasm and progenies in F2and F3[J].Heilongjiang Agricultural Sciences，1995，1：6-8）
[6]张思河，王萍，马淑英，等.三个熟期类型大豆种子萌发进程中耐冷性的比较[J].大豆科学，2000，19（3）：218-222.（Zhang S H，Wang P，Ma S Y，et al.Comparative study on the cold tolerance of three maturity type soybean seeds during the course of germination[J].Soybean Science，2000，19(3)：218-222.）
[7]郝晶，张立军，谢甫绨.低温对大豆不同耐冷性中萌发期保护酶活性的影响[J].大豆科学，2007，26（2）：171-175.（Hao J，Zhang L J，Xie F D.Effects of the low temperature on defense enzyme activities of different chilling tolerant soybean cultivars during the germination[J].Soybean Science，2007，26(2)：171-175.）
[8]马淑英，尹田夫，宋海星，等.低温对不同耐冷大豆萌发种子游离脯氨酸变化的影响[J].吉林农业科学，1998，4：88-90.（Ma S Y，Yin T F，Song H X，et al.The effect of low temperature on the change of the free proline content in soybean seeds[J].Jilin Agricultural Science，1998，4：88-90.）
[9] Ga′bor Kocsy，Bala′zs To′th，Tama′s Berzy，et al.Glutathione reductase activity and chilling tolerance are induced by a hydroxylamine derivative BRX-156 in maize and soybean[J].Plant Science，2001，160：943-950.
[10]Van Heerden P D R,VIljoen M M,De Villiers M F，et al.Limitation of photosynthetic carbon metabolism by dark chilling in temperate and tropical soybean genotypes[J].Plant Physiology and Biochemistry，2004，42：117-124.
[11]任丽丽，高辉远.低温弱光胁迫对野生大豆和大豆栽培种光系统功能的影响[J].植物生理与分子生物学学报，2007，33（4）：333-340.（Ren L L，Gao H Y.Effects of chilling stress under weak light on functions of photosystems in leaves of wild soybean and cultivator soybean[J].Journal of Plant Physiology and Molecular Biology，2007，33(4)：333-340.）
[12]盖钧镒，张元明，王健康.植物数量遗传体系[M].北京：科学出版社，2003：224-265.（Gai J Y，Zhang Y M，Wang J K.Genetic system of quantitative traits in plants [M].Beijing：Sience Press，2003:224-265.）
[13]翟虎渠.应用数量遗传学[M].北京：中国农业出版社，2001.（Zhai H Q.Applied genetics of quantitative traits [M].Beijing:China Agric Press，2001.）
[14]徐吉臣，邹亮星.利用相关性分析鉴定与水稻根部性状表达相关的分子标记[J].遗传学报，2002，29（3）：245-249.（Xu J C，Zhou L X.Indentification of molecular markers associated with rice root traits by correlation coefficient analysis[J].Acta Genetica Sinica，2002，29(3)：245-249.）

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(06):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(06):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(06):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(06):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(06):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(06):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(06):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(06):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(06):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(06):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

基金项目：教育部长江学者和创新团队发展计划项目(IRT0432)。

作者简介：胡国玉(1977-)，女，硕士研究生，主要从事大豆遗传与作物遗传育种研究。 E-mail: gy_hu@126.com。

更新日期/Last Update: 2014-10-04