[1]李晓梅.大豆茎顶端分生组织石蜡切片的制备[J].大豆科学,2008,27(04):708-710.[doi:10.11861/j.issn.1000-9841.2008.04.0708]
　LI Xiao-mei.Manufaction of Paraffin Section on Apical Meristem of Soybean[J].Soybean Science,2008,27(04):708-710.[doi:10.11861/j.issn.1000-9841.2008.04.0708]
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大豆茎顶端分生组织石蜡切片的制备

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年04期 页码: 708-710 栏目: 出版日期: 2008-08-25
Title:
Manufaction of Paraffin Section on Apical Meristem of Soybean
文章编号:
1000-9841(2008)04-0708-03
作者:
李晓梅
枣庄学院生命科学系，山东 枣庄 277160
Author(s):
LI Xiao-mei
Department of Biology Science，Zaozhuang University，Zaozhuang 277160，Shandong，China
关键词:
大豆; 顶端分生组织; 石蜡切片
Keywords:
Soybean; Apical meristem; Paraffin section
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0708
文献标志码:
A
摘要:
为探讨大豆茎顶端分生组织石蜡切片的制作方法,观察大豆茎顶端发育过程的微观结构变化。将大豆顶芽经固定、脱水、透明、浸蜡、包埋、切片、染色等一系列过程，获得其纵切片。结果表明：生长锥顶端、叶原基、花原基等分裂活跃的部位染色较深,髓分生组织部位染色较浅；在营养生长阶段，叶原基依次从茎顶端分生组织底部发育，逐渐变大；在生殖生长阶段，顶端变成伸长的圆顶形，然后开始分化各轮花器官。研究结果可为深入观察大豆茎顶端分生组织解剖结构及分生组织发育的分子生物学研究提供基础。
Abstract:
In order to obtain more microstructure information on development of soybean，the methods for apical meristem sections were studied in the paper.Based on the process of fixation，dehydration，transparency，saturated paraffin，embedding，section and dye，straight-sections of the shoot apical meristem of soybean were obtained.The results from this study indicated that the regions splitting actively，such as the top of apical meristem，leaf primordia and flower primordia，were dyed deeply.At the same time，pith meristem zone was dyed lightly.At vegetative growth stage，leaf primordia were found at the basal part of apical meristem，and they became bigger gradually.At reproductive growth stage，the dome-liked apical meristem became longer slightly，then each of floral primordia began to appear in turn.The results could provide the base for further investigation on anatomical structure and developmental biology of shoot apical meristem in soybean.

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备注/Memo

基金项目：国家自然科学基金资助项目（30070456）。

作者简介：李晓梅（1972-），女，硕士，主要从事作物生理研究工作。E-mail：elixiaomei@163.com。

更新日期/Last Update: 2014-10-07