[1]李蕊,冯海涛,郭东林,等.硝基苯诱发大豆根尖细胞遗传畸变研究[J].大豆科学,2008,27(03):539-542.[doi:10.11861/j.issn.1000-9841.2008.03.0539]
　LI Rui,Feng Hai-tao,GUO Dong-lin,et al.Genetic Aberration on Root Tip Cells of Soybean （Glycine max）Induced by Nitrobenzene[J].Soybean Science,2008,27(03):539-542.[doi:10.11861/j.issn.1000-9841.2008.03.0539]
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硝基苯诱发大豆根尖细胞遗传畸变研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 539-542 栏目: 出版日期: 2008-06-25
Title:
Genetic Aberration on Root Tip Cells of Soybean （Glycine max）Induced by Nitrobenzene
文章编号:
1000-9841(2008)03-0539-04
作者:
李蕊1; 冯海涛1; 郭东林1; 陈国友2; 郭长虹1
1哈尔滨师范大学生命与环境科学学院生物学系，黑龙江 哈尔滨150025；
2黑龙江省农业科学院谷物品质研究中心，黑龙江 哈尔滨150086
Author(s):
LI Rui1; Feng Hai-tao1; GUO Dong-lin1; CHEN Guo-you2; GUO Chang-hong1
1College of Biology and Environment，Harbin Normal University，Harbin 150025；
2Center of Cereal Quality Research，Heilongjiang Academy of Agricultural Science，Harbin 150086，Heilongjiang，China
关键词:
硝基苯; 大豆; 遗传畸变
Keywords:
Nitrobenzene; Soybean(Glycine max); Genetic aberration
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0539
文献标志码:
A
摘要:
硝基苯是生产苯胺、联苯胺、二硝基苯的过程中的中间体，也是许多商业产品的主要成分。为了研究硝基苯对作物的遗传毒性，以大豆为试验材料，用硝基苯溶液处理大豆根部，进行了微核试验和染色体畸变试验。结果表明：硝基苯可以诱发多种类型的染色体畸变，主要有间期的微核、双核；中期的染色体断片；后期的染色体桥、染色体落后等。对硝基苯的诱变机理进行了分析，认为硝基苯可能是直接与DNA作用引起其断裂，也可能是通过激发细胞内的自由基反应而导致染色体畸变。
Abstract:
Nitrobenzene is an intermediate in the synthesis of aniline，benzidine and dinitrobenzene，and it is also a component in a number of commercial products.Nitrobenzene has been shown to induce cancers in many tissues of animals.In order to study the mutant effect of soybean root tip cells induced by Nitrobenzene，the abnormity was observed through plant cell micronucleus assay and chromosomal aberration test assay.The result showed that Nitrobenzene induced various types of chromosome aberration on soybean root tips，such as micronuclear and binuclear in interphase;chromosome fragments in metaphase；chromosome bridges and laggard chromosomes in anaphase.The mechanism of chromosomal aberration is probable that Nitrobenzene arise DNA breakage directly or excite free radical reaction in cells indirectly.

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备注/Memo

基金项目：国家科技攻关资助项目(2006BA618A);黑龙江省科技攻关资助项目；哈尔滨师范大学骨干教师资助计划项目(KG2006-05); 哈尔滨师范大学大学生科技创新基金资助项目(2007046)。

作者简介：李蕊（1983-），女，硕士研究生，主要从事植物遗传学研究。冯海涛为共同第一作者。

更新日期/Last Update: 2014-10-08