[1]刘丽君,孙欣,薛永国,等.大豆抗疫霉根腐病基因SSR标记[J].大豆科学,2008,27(03):379-382.[doi:10.11861/j.issn.1000-9841.2008.03.0379]
　LIU Li-jun,SUN Xin,XUE Yong-guo,et al.Tagging SSR Markers for Resistant Gene to Phytophthora Root Rot in Soybean[J].Soybean Science,2008,27(03):379-382.[doi:10.11861/j.issn.1000-9841.2008.03.0379]
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大豆抗疫霉根腐病基因SSR标记

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 379-382 栏目: 出版日期: 2008-06-25
Title:
Tagging SSR Markers for Resistant Gene to Phytophthora Root Rot in Soybean
文章编号:
1000-9841(2008)03-0379-04
作者:
刘丽君; 孙欣; 薛永国; 杨喆
黑龙江省农业科学院大豆研究所 黑龙江 哈尔滨150086
Author(s):
LIU Li-jun; SUN Xin; XUE Yong-guo; YANG Zhe
Soybean Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆;  疫霉根腐病;  SSR;  BSA
Keywords:
Soybean Phytophthora root rot;  SSR; BSA
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0379
文献标志码:
A
摘要:
选用感病大豆品种合丰25和抗病大豆品种抗线2号作为亲本配制杂交组合，获得F2种子。种植F2代种子获得F2：3家系，作为分子标记分离群体，进行大豆抗疫霉根腐病基因SSR标记。共筛选覆盖大豆全基因组的350对SSR引物，其中52对引物在亲本间具有多态性，在F2群体中通过BSA法筛选出与抗疫霉病基因相关的多态性差异引物1对，为Scaa003，位于大豆公共遗传图谱的J连锁群。同时该引物在其他10个抗病品种及4个感病品种中抗、感病谱带检出率均为100%，具有一定的检测通用性，可以为大豆疫霉根腐菌1号生理小种抗性材料的辅助选择提供理论依据。
Abstract:
Phytophthora root rot is an important soybean disease，which can influence the yield.To map SSR makers link with resistant genes to soybean Phytophthora root rot，Hefeng 25 and Kangxian 2 were crossed and the F2 seperation population was obtained.350 pair of SSR primers were screened and 52 pairs have polymorphism.BSA(Bulked segregation analysis) method was used to screen the polymorphic and diverge primers，one pair of polymorphic primer，Scaa003，located on LG J was tagged.The examination rate of Scaa003 between 10 sensitive soybean cultivars and 4 resistant soybean cultivars reached 100%.Result shows that the primer has the common characteristic for the examination，it can provide the theoretic base for selecting the soybean materials which have the resistance to the soybean Phytophthora sojae Race 1.

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备注/Memo

基金项目：国家高技术研究发展计划“863” 计划资助项目(2006AA10Z1F9)。

作者简介：刘丽君（1958-）女，研究员，研究方向为分子植物育种。E-mail: nkyssbd@126.com。

更新日期/Last Update: 2014-10-07