[1]王俊,王林林,刘章雄,等.大豆地方品种遗传结构及其保存研究[J].大豆科学,2008,27(03):361-365.[doi:10.11861/j.issn.1000-9841.2008.03.0361]
　WANG Jun,WANG Lin-lin,LIU Zhang-xiong,et al.Genetic Structure and Conservation of Soybean Landraces[J].Soybean Science,2008,27(03):361-365.[doi:10.11861/j.issn.1000-9841.2008.03.0361]
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大豆地方品种遗传结构及其保存研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年03期 页码: 361-365 栏目: 出版日期: 2008-06-25
Title:
Genetic Structure and Conservation of Soybean Landraces
文章编号:
1000-9841(2008)03-0361-05
作者:
王俊1; 2; 王林林1; 2; 刘章雄2; 关荣霞2; 金龙国2; 李英慧2; 常汝镇2; 罗淑萍1; 邱丽娟2
1新疆农业大学农学院，新疆 乌鲁木齐 830052；
2中国农业科学院作物科学研究所/国家农作物基因资源与遗传改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京 100081
Author(s):
WANG Jun1; 2; WANG Lin-lin1; LIU Zhang-xiong2; GUAN Rong-xia2; JIN Long-guo2; LI Ying-hui2; CHANG Ru-zhen2; LUO Shu-ping1; QIU Li-juan2
1Agronomy College of XinJiang Agricultural University，Urumqi 830052，Xinjiang；
2The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) / Key Laboratory of Germplasm & Biotechnology (MOA)，Institute of Crop Sciences，Chinese Academy of Agricultural Sciences，Beijing 100081，China
关键词:
大豆; 地方品种; SSR; 纯度鉴定; 遗传完整性; 品种保存
Keywords:
Soybean; Landrace; SSR; Purity determination; Genetic integrity; Variety preservation
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0361
文献标志码:
A
摘要:
我国具有丰富的地方品种，但在大豆育种中利用率较低，这与对地方品种的保存和鉴定研究较少有关。通过利用全基因组SSR标记扫描的方法对地方品种羊眼豆和毛豆进行纯度鉴定，旨在为地方品种的纯化及遗传完整性的保存提供科学依据。结果表明：羊眼豆在502个SSR标记位点的多态性为44.8%，毛豆在405个SSR标记位点的多态性为41.2%；检测2个地方品种纯度所需的最少标记数和种子粒数分别为3个标记和4粒种子；根据遗传一致性分别对2个地方品种进行聚类，在相似系数为0.85时，均被分为3个组。因此，这2个地方品种纯度较低，根据遗传一致性分组情况，对两个地方品种的一组进行提纯后作为一个品种保存，而另外2个组则可分别作为2个不同的地方品种保存其遗传完整性。
Abstract:
There are abundant soybean landraces in China，but few of them has been utilized in breeding program because of insufficient identification.In this study，two landraces of Yangyandou and Maodou were analyzed for their identity in order to provide instruction of purification and genetic integrity conservation of soybean landraces.The genome-wide SSR markers screening was used to analyze two landraces.Results showed(ⅰ) 44.8% of the 502 selected SSR markers showed polymorphism in Yangyandou and 167 of the 405 SSR markers showed polymorphism which accounted for 41.2% in Maodou；(ⅱ) The minimum numbers of SSR markers and individual seed to determine the purity of two landraces were 3 and 4 respectively；(ⅲ) Based on the cluster analysis，the two landraces could be segregated into 3 groups when the identity coefficient was at the level of 0.85.Results suggest that the purity of the two landrace are at low level.According to the genetic identity，one of the three groups could be utilized for one landrace and the others two groups could be separated as two different genetic integrity preservations for each of two landraces.

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备注/Memo

基金项目：科技部大豆共享数据平台课题资助项目（2005DKA21001）；农业部大豆繁种课题资助项目（NB06-070401-05，NB05-070401-22-06，B06-070401-18-11）。

作者简介：王俊（1981-），男，硕士研究生，研究方向为大豆种质资源。E-mail：wjsoybean_2008@163.com。

更新日期/Last Update: 2014-10-07