[1]张淑珍,徐鹏飞,吴俊江,等.大豆疫霉根腐病菌毒素对抗感不同大豆品种叶片超微结构的影响[J].大豆科学,2008,27(02):288-291.[doi:10.11861/j.issn.1000-9841.2008.02.0288]
　ZHANG Shu-zhen,XU Peng-fei,WU Jun-jiang,et al.Effect of Pathotoxin Produced from Phytophthora sojae on the Ultrastructure of Leaves of Soybean Varieties with Different Resistance[J].Soybean Science,2008,27(02):288-291.[doi:10.11861/j.issn.1000-9841.2008.02.0288]
点击复制

大豆疫霉根腐病菌毒素对抗感不同大豆品种叶片超微结构的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 288-291 栏目: 出版日期: 2008-04-30
Title:
Effect of Pathotoxin Produced from Phytophthora sojae on the Ultrastructure of Leaves of Soybean Varieties with Different Resistance
文章编号:
1000-9841(2008)02-0288-04
作者:
张淑珍1; 徐鹏飞1; 吴俊江2; 李文滨1; 陈维元3; 于安亮1; 王金生1; 陈晨; 靳立梅1; 李岑
1东北农业大学大豆研究所，大豆生物学教育部重点实验室，黑龙江 哈尔滨150030；
2黑龙江省农业科学院大豆研究所，黑龙江 哈尔滨150086；
3黑龙江省农业科学院绥化所，黑龙江 绥化152052
Author(s):
ZHANG Shu-zhen1; XU Peng-fei1; WU Jun-jiang2; LI Wen-bin1; CHEN Wei-yuan3; YU An-liang1;  WANG Jin-sheng1; CHEN Chen1;  JIN Li-mei1; LI Cen1
1Soybean Research Institute，Northeast Agricultural University;Chinese Education Ministry’s Key Laboratory of Soybean Biology，Harbin 150030，Heilongjiang；
2 Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences（HAAS)，Harbin 150086，Heilongjiang；
3 Suihua Research Institute of HAAS，Suihua 152052，Heilongjiang，China
关键词:
大豆疫霉根腐病; 毒素; 抗感品种; 超微结构
Keywords:
Phytophthora sojae; Pathotoxin; Varieties with different resistance;  Ultrastructure
分类号:
S435.651
DOI:
10.11861/j.issn.1000-9841.2008.02.0288
文献标志码:
A
摘要:
毒素是大豆疫霉根腐病的主要致病因子之一，而毒素对大豆细胞超微结构的影响还未见报道，用不同浓度的毒素处理抗感不同大豆品种的叶片，为揭示毒素在细胞水平的致病机理及寄主抗毒素的机理奠定一定的理论基础。结果表明，较高浓度大豆疫霉根腐病菌毒素（稀释50倍，浓度为0.1794 mgmL-1）处理抗感不同大豆品种的离体叶片后，不论是抗病品种还是感病品种叶片细胞内部结构都遭到了明显的破坏。适宜浓度的毒素（稀释100倍，浓度为0.0897 mgmL-1）处理抗感品种离体叶片后，抗病品种细胞内部结构基本上比较完整，而感病品种叶片细胞内部结构已经发生了较大的变化，叶绿体嵴变形，基粒片层已经基本消失，叶绿体膜已经解体，线粒体空泡化严重。说明只有在适宜浓度毒素处理下，才能在细胞超微结构上区分出抗感品种在抗性上的差异。
Abstract:
Pathotoxin is one of the main pathogenic factors of Phytophthora sojae，which could destroy the cell structure of soybean.To investigate the effect of pathotoxin on cell ultrastructure of soybean，two soybean varieties of Suinong 10(resistant to Phytophthora sojae) and Hefeng 25(susceptible to Phytophthora sojae) were used in this experiment.The excised leaves of the two soybean varieties were dipped into the pathotoxin solution with different concentration（one is the pathotoxin solution of relatively high concentration，0.1794 mgmL-1；the other is pathotoxin solution of optimal concentration，0.0897 mgmL-1），and fixed in the fixation solution after 48 h and 72 h.The ultrastructures of leaves of Suinong 10 and Hefeng 25 were all seriously destroyed after treated with relatively high concentration of pathotoxin solution.The ultrastructure of leaves of resistant soybean Suinong 10 was almost intact after treated with optimal concentration of pathotoxin，but the cell structure of susceptible variety Hefeng 25 changed a lot.The chloroplast grana nearly disappeared，chondriosome appeared vacuolus，and chondriosome envelope disaggregated.The results showed that only optimal concentration of pathotoxin solution could distinguish the difference in cell structure between soybean varieties resistant or susceptible to Phytophthora sojae.

参考文献/References:

[1] Schmitthenner A F.Problems and progress in control of Phytophthora root rot of soybean[J].Plant Disease，1985,69(4)：362-368.

[2] Hildebrand A A.A root and stalk rot of soybeans caused by Phytophthora Megasperma Drechsler var.sojae var［J］.Canadian Journal of Botany，1959,37：927-957.
[ 3] Pegg K G，Kochman J K，Vock N T.Root and stem rot of soybean caused by Phytophthora megasperma var.sojae[J].Australasian Plant Pathology，1980,9:15.
[4] Ryley M J，Obst N R，Irwin J A G，et al.Changes in the racial composition of Phytophthora sojae in Australia between 1979 and 1996[J].Plant Disease，1998,82:1048-1054.
[5] Jee H，Kim W，Cho W.Occurance of Phytophthora root rot on soybean（Glycine max) and indentification of the causal fungus[J].Journal of Crop Protection，1998,40(1)：16-22.
[6] Wrather J A，Anderson T R，Arsyad D M，et al.Soybean disease loss estimates for the top ten soybean-producing countries in 1998[J].Canadian Journal Plant Pathology，2001,23:115–121.
[7]沈崇尧，苏彦纯.中国大豆疫霉病菌的发现及初步研究[J].植物病理学报，1991,21(3)：298.(Shen C Y，Su Y C.Discovery and preliminary studies of phytophthora megasperma on soybean in China[J].Acta Phytopathologica Sinica，1991,21(3)：298.)
[8]韩晓增，何志鸿，张增敏.大豆主要病虫害防治技术[J].大豆通报，1998,(6):5-6.(Han X Z，He Z H，Zhang Z M.Extermination of disease and insect pest[J].Soybean Bulletin，1998,(6):5-6.)
[9]马淑梅，李宝英.大豆疫霉根腐病菌生理小种鉴定结果初报[J].大豆科学，1999, 18(2)：151-153.(Ma S M，Li B Y.A preliminary report on the identification of the physiological races of phytophthora megasperma［J］.Soybean Science，18(2)：151-153.)
[10]许修宏，吕慧颖，杨庆凯，等.大豆疫霉根腐病抗源筛选[J].大豆科学，1999,18（2）：147-150.(Xu X H，Lv H Y，Yang Q K，et al.Screening of soybean germplasms for resistance to Phytophthora sojae[J].Soybean Science，1999,18（2）:147-150.)
[11]章元寿.植物病理生理学[M].南京：江苏科学技术出版社，1994：50(Zhang Y S.Plant pathology physiology[M].Nanjing:Jiangsu Science and Technology Press，1994:50.)
[12]张淑珍，吴俊江，葛秀秀，等.大豆疫霉菌产毒条件的研究[J].植物病理学报，2002,32（1）：89-90.(Zhang S Z，Wu J J，Ge X X，et al.Factors affecting toxin production of phytophthora sojae［J］.Acta Phytopathologica Sinica，2002,32(1)：89-90.)
[13]张淑珍，徐鹏飞，韩英鹏，等.大豆疫霉菌毒素处理大豆品种后几丁质酶活性的变化[J].作物杂志，2006,（3）：17-19(Zhang S Z，Xu P F，Han Y P，et al.The chitinase activity of soybean varieties after treated with pathotoxin produced from Phytophthora sojae[J].Crops，2006,(3)：17-19.)
[14]张淑珍，徐鹏飞，武小霞，等.大豆疫霉根腐病菌毒素的提取及生物活性测定[J].中国农学通报，2005,21（3）：252-255.(Zhang S Z，Xu P F，Wu X X,et al.Study on extraction and bioactivity assay of pathotoxin produced by Phytophthora sojae[J].Chinese Agricultural Science Bulletin，2005,21(3)：252-255.)
[15]章元寿，王建新.棉花黄萎病菌毒素产生条件的研究[J].南京农业大学学报，1988,11（3）：57-62.(Zhang Y S，Wang J X.Studies on producing condition of the toxin prom verticillium dahliae kleb.causing verticillium wilt of cotton[J].Journal of Nanjing Agricultural University，1988,11(3)：57-62.)

相似文献/References:

[1]韩英鹏,程 章,赵 雪,等.大豆花叶病毒病和疫霉根腐病抗性的SSR标记辅助鉴定[J].大豆科学,2013,32(06):740.[doi:10.11861/j.issn.1000-9841.2013.06.0740]
[2]程鹏,徐鹏飞,范素杰,等.野生大豆接种大豆疫霉根腐病菌后过氧化物酶（POD）活性变化[J].大豆科学,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
　CHENG Peng,XU Peng-fei,FAN Su-jie,et al.Response of POD Activity in Glycine soja ?Inoculated by Phytophthora sojae[J].Soybean Science,2013,32(02):197.[doi:10.3969/j.issn.1000-9841.2013.02.013]
[3]韩英鹏,赵 雪,李修平,等.大豆种质对花叶病毒病和疫霉根腐病抗病性的SSR标记辅助鉴定[J].大豆科学,2014,33(01):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
　HAN Yingpeng,ZHAO Xue,LI Xiuping,et al.SSR Identification of Soybean Cultivar with Resistance to Soybean Mosaic Virus and Phytophthpra Root Rot[J].Soybean Science,2014,33(02):27.[doi:10.11861/j.issn.1000-9841.2014.01.0027]
[4]申宏波,姚文秋,于永梅,等.不同类型生物农药对大豆疫霉根腐病的防治效果[J].大豆科学,2011,30(06):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
　SHEN Hong-bo,YAO Wen-qiu,YU Yong-mei,et al.Control Efficiency of Different Biopesticides on Soybean Phytophthora Root Rot[J].Soybean Science,2011,30(02):1054.[doi:10.11861/j.issn.1000-9841.2011.06.1054]
[5]张淑珍,靳立梅,徐鹏飞,等.野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶（PAL）活性的变化[J].大豆科学,2009,28(06):1044.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
　ZHANG Shu-zhen,JIN Li-mei,XU Peng-fei,et al.Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja[J].Soybean Science,2009,28(02):1044.[doi:10.11861/j.issn.1000-9841.2009.06.1044]
[6]任龙翚,张宝强,武晓玲,等.大豆种质对大豆疫霉菌株Pm8的抗性分析[J].大豆科学,2010,29(01):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
　REN Long-hui,ZHANG Bao-qiang,WU Xiao-ing,et al.Resistance of Soybean Germplasm to Phytophthora sojae Pm8[J].Soybean Science,2010,29(02):77.[doi:10.11861/j.issn.1000-9841.2010.01.0077]
[7]徐鹏飞,吴俊江,范素杰,等.大豆疫霉根腐病菌的分离鉴定及种质资源对3号生理小种的抗性评价[J].大豆科学,2010,29(02):272.[doi:10.11861/j.issn.1000-9841.2010.02.0272]
　XU Peng-fei,WU Jun-jiang,FAN Su-jie,et al.Isolation of Phytophthora sojae and Identification of Resistance to Race 3 in Soybean Germplasm[J].Soybean Science,2010,29(02):272.[doi:10.11861/j.issn.1000-9841.2010.02.0272]
[8]马淑梅,金娜,邵红涛.黑龙江省大豆疫霉根腐病菌毒力类型分布及品种抗性评价[J].大豆科学,2010,29(03):466.[doi:10.11861/j.issn.1000-9841.2010.03.0466]
　MA Shu-mei,JIN Na,SHAO Hong-tao.Virulence Distribution and Cultivars Resistance Evaluation of Phytophthora Megasperma in Heilongjiang Province[J].Soybean Science,2010,29(02):466.[doi:10.11861/j.issn.1000-9841.2010.03.0466]
[9]任海龙,宋恩亮,马启彬,等.南方三省（区）抗大豆疫霉根腐病野生大豆资源的筛选[J].大豆科学,2010,29(06):1012.[doi:10.11861/j.issn.1000-9841.2010.06.1012]
　REN Hai-long,SONG En-liang,MA Qi-bin,et al.Screening for Resistance Sources to Phytophthora Root Rot in Glycine soja from Three Provinces of Southern China[J].Soybean Science,2010,29(02):1012.[doi:10.11861/j.issn.1000-9841.2010.06.1012]
[10]于安亮,徐鹏飞,陈晨,等.大豆疫霉根腐病子叶接种法抗病性鉴定[J].大豆科学,2009,28(05):879.[doi:10.11861/j.issn.1000-9841.2009.05.0879]
　YU An-liang,XU Peng-fei,CHEN Chen,et al.Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method[J].Soybean Science,2009,28(02):879.[doi:10.11861/j.issn.1000-9841.2009.05.0879]
[11]程莹鑫,梁吉利,刘庆莉,等.疫霉根腐病菌毒素对大豆不同组织中总多酚含量的影响[J].大豆科学,2014,33(04):524.[doi:10.11861/j.issn.1000-9841.2014.04.0524]
　CHENG Ying-xin,LIANG Ji-li,LIU Qing-li,et al.Changes of Total Polyphenol Content in Soybean Treated by Pathotoxin Produced by Phytophthora sojae[J].Soybean Science,2014,33(02):524.[doi:10.11861/j.issn.1000-9841.2014.04.0524]

备注/Memo

基金项目：国家自然科学基金资助项目（30400285，30671317）；中国博士后基金资助项目（20060400835）；黑龙江省教育厅新世纪人才基金资助项目（NCET-06-007）；黑龙江省高校骨干教师资助项目项目（1151G007）；黑龙江省农科院博士后基金资助项目（LRB06-010）；农业部寒地重点开放实验室开放课题，博士后落户黑龙江启动基金资助项目（LBH-Q05032）；黑龙江省青年基金资助项目（QC06C012）。

作者简介：张淑珍（1972-），女，博士，副教授，研究方向为大豆抗病遗传育种。E-mail: dnzhshzh@yahoo.com.cn ；Tel:0451-55191487。

更新日期/Last Update: 2014-10-10