[1]徐凌翔,丁爽,周青.大豆种子萌发过程中呼吸作用对酸雨胁迫的响应[J].大豆科学,2008,27(02):255-258,266.[doi:10.11861/j.issn.1000-9841.2008.02.0255]
　XU Ling-xiang,DING Shuang,ZHOU Qing.Reponses of Respiration in Seed Germination of Soybean to Acid Rain Stress[J].Soybean Science,2008,27(02):255-258,266.[doi:10.11861/j.issn.1000-9841.2008.02.0255]
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大豆种子萌发过程中呼吸作用对酸雨胁迫的响应

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 255-258,266 栏目: 出版日期: 2008-04-30
Title:
Reponses of Respiration in Seed Germination of Soybean to Acid Rain Stress
文章编号:
1000-9841(2008)02-0255-04
作者:
徐凌翔2; 丁爽2; 周青1; 2
1 江南大学工业生物技术教育部重点实验室，江苏 无锡 214122；
2 江南大学环境与土木工程学院，江苏 无锡 214122
Author(s):
XU Ling-xiang2; DING Shuang2; ZHOU Qing1; 2
1Key Laboratory of Industrial Biotechnology，Ministry of Education，Jiangnan University，Wuxi 214122;
2 School of Environmental and Civil Engineering，Jiangnan University，Wuxi 214122，Jiangsu，China
关键词:
酸雨; 大豆种子; 呼吸作用
Keywords:
ean seed; Respiration
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0255
文献标志码:
A
摘要:
酸雨对作物种子萌发的生态效应是影响农业生产的重要问题。为揭示酸雨影响种子萌发机理，以pH 2.0、2.5、3.0、3.5、4.0、4.5、5.0模拟酸雨处理大豆种子，研究发芽率、发芽势、发芽指数、呼吸速率、呼吸商、POD活性对酸雨胁迫的响应。结果表明：当酸雨胁迫强度pH＝2.0时，种子不能萌发；胁迫强度pH≥2.5时各处理皆可正常萌发，但pH 2.5时出苗率(6 d)为0，各生理指标在第1天出现异常，POD表现出了参与衰老迹象；胁迫强度pH≥3.0时，大豆种子呼吸强度随胁迫强度升高缓慢下降，动态处理仅有1 d达到差异显著水平；呼吸商随种子成苗逐步降低，与CK趋势不同；POD活性随胁迫强度和时间增加逐步升高，各处理与pH 2.5差异显著。呼吸作用伤害阈值( pH≥5.0) =大豆POD活性伤害阈值(pH≥5.0)＞萌发伤害阈值(pH≥3.0)。pH 3.5、3.0和2.5时，呼吸速率响应时间(3 d、3 d、1 d) >呼吸商响应时间(2 d、2 d、1 d)>POD活性响应时间(1 d、1 d、1 d)。由此推测，酸雨胁迫引起自由基积累，分散呼吸链底物电子，抑制呼吸；呼吸链末端抗氧化酶POD在清除自由基的同时参与了种子衰老。
Abstract:
As an important problem affecting agricultural productivity，ecological effect of acid rain on crop seeds germination is highly concerned by researchers at domestic and abroad.Effects of acid rain on soybean germination and respiration were investigated to reveal the mechanism of acid rain on seed germination.Soybean seeds of Shuangqingdou as experimental material，six simulated acid rain（SAR) solutions of pH 2.0，2.5，3.0，3.5，4.0 and 5.0，and neutral solution（pH 6.5) as control group（CK) were used in this study.Pretreated seeds of soybean were placed in culture dishes with filter sheets to germinate in normal temperature，three dishes were involved in each treatment group and 12 seeds were placed in each dish.During germination，seeds were exposed to simulated acid rain that was replaced everyday.The germination indexes（percentage germination，germination energy，germination index)，respiration indexes（respiratory rate（RR)，respiratory quotient（RQ)) and activity of peroxidase(POD)were all studied under acid rain stress.Seed germination was inhibited significantly in high acidity strength（pH 2.0).When pH≥2.5，each solution containing CK had good germination index，but rate of emergence（6 d) was zero and all physiological indexes（1 d) were all abnormal，POD took part in aging.When pH≥3.0，RR decreased slowly with the decreasing pH，and the stress time when RR was significant different from CK was 1 d；RQ decreased as seeds grew to seedlings，and was different from CK；POD activity gradually increased with prolonging stress time and stress strength，and was different from pH 2.5.The threshold value of respiration( pH≥5.0)was the same as that of activity of POD(pH≥5.0)and lower than that of germination（pH≥3.0).Under pH 2.5，3.0，3.5，the stress time when RR（1 d，3 d，3 d)was different from that of CK，and higher than that of POD activity（1 d，1 d，1 d) and RQ（1 d，2 d，2 d).So it could be speculated that stress of acid rain caused stimulation of free radical，deprived electrons on substrates of respiratory chain，and then inhibited respiration；POD，which located in the end of respiratory chain，scavenged free radical and also participated in seed aging.

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备注/Memo

基金项目：江苏省教育厅“江苏省高等学校大学生实践创新训练”资助项目。

作者简介：徐凌翔(1984-),男,硕士，研究方向为作物种子萌发阶段对酸雨胁迫的响应。Ｅ-mail:xlxmy@hotmail.com。

更新日期/Last Update: 2014-10-10