[1]唐晓飞,刘丽君,张小明,等.高产大豆新品系哈交5337和哈交5489再生条件的优化[J].大豆科学,2008,27(02):203-207.[doi:10.11861/j.issn.1000-9841.2008.02.0203]
　TANG Xiao-fei,LIU Li-jun,ZHANG Xiao-ming,et al.Improvement of Regeneration System in High-yield Soybean Lines Hajiao 5337 and Hajiao 5489[J].Soybean Science,2008,27(02):203-207.[doi:10.11861/j.issn.1000-9841.2008.02.0203]
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高产大豆新品系哈交5337和哈交5489再生条件的优化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年02期 页码: 203-207 栏目: 出版日期: 2008-04-30
Title:
Improvement of Regeneration System in High-yield Soybean Lines Hajiao 5337 and Hajiao 5489
文章编号:
1000-9841(2008)02-0203-05
作者:
唐晓飞1; 2; 刘丽君2; 张小明1; 2; 薛永国1; 2; 杨喆2; 高明杰2; 张雷2; 魏崃1
1东北农业大学研究生院，黑龙江 哈尔滨 150030；
2黑龙江省农业科学院大豆研究所，黑龙江 哈尔滨 150086
Author(s):
TANG Xiao-fei1; 2; LIU Li-jun2; ZHANG Xiao-ming1; 2; XUE Yong-guo1; 2; YANG Zhe2; GAO Ming-jie2; ZHANG Lei2; WEI Lai1
1Graduate School of Northeast Agricultural University，Harbin 150086，Heilongjiang;
2 Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
关键词:
大豆; 子叶节; 分化率
Keywords:
Soybean; Cotyldonary nodes; Rate of shooting
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.02.0203
文献标志码:
A
摘要:
为了拓宽大豆受体材料基因型，提高转基因大豆的应用价值，对两个综合性状较好的新品系大豆哈交5337和哈交5489进行子叶节器官发生途径再生条件的优化研究。在芽诱导培养基中添加不同浓度6-BA和IBA，采用正交法进行分析，取含6-BA（G1）萌发培养基中的大豆子叶节与不含6-BA（G2）萌发培养基中的大豆子叶节做平行对照，结果表明：哈交5337最适的萌发培养基与芽诱导培养基的组合为G1S7（萌发培养基中不添加6-BA，芽诱导培养基中添加1.7 mgL-16-BA和0.1 mgL-1IBA）和G2S4（萌发培养基中添加1.0 mgL-1的6-BA，芽诱导培养基中添加1.1mgL-16-BA和0.1 mgL-1IBA）；哈交5489最适的萌发培养基与芽诱导培养基的组合为G1S4（萌发培养基中不添加6-BA，芽诱导培养基中添加1.1 mgL-16-BA和0.1 mgL-1IBA）和G2S4（萌发培养基中添加1.0 mgL-1的6-BA，芽诱导培养基中添加1.1 mgL-16-BA和0.1 mgL-1IBA）；同时确定两个品系大豆在丛生芽分化阶段采用延迟筛选方法，草铵膦筛选浓度为3.5 mgL-1。
Abstract:
Plant transformation is an important approach in genetic engineering breeding，great advances have been achieved in soybean transformation recently. In order to broaden soybean receptor genotypes and enhance the application value of transgenic soybean，new soybean lines Hajiao 5337 and Hajiao 5489 were used to optimize the regeneration of the cotyledon node organ. Different concentration of 6-BA and IBA were used in the shoot induction medium，the ratio of shooting was analyzed using the orthogonal analysis. The cotyledon node grown in the germinated medium with 6-BA（named G1)and the germinated medium without 6-BA（named G2)were used as controls，respectively. It indicated that G1S7 and G2S4 was the optimum combination for the cotyledons of Hajiao 5337 in germinating medium and shooting induction medium，while G1S4 and G2S4 for Hajiao 5489. The adoption of the delay screening，the optimal screening concentration of Glufosinate-ammonium to Hajiao 5337 and Hajiao 5489 was 3.5 mgL-1in shooting induction medium.

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备注/Memo

基金项目：国家高技术研究发展计划项目资助（2006AA1021F9）；国家大豆产业技术体系资助。

作者简介：唐晓飞(1981-)，女，硕士研究生，研究方向为分子育种。E-mail:xftang@126.com。

更新日期/Last Update: 2014-10-10