[1]孙庆元,张雪崧,赵略,等.尿素酶抑制剂NBPT对大豆根系的影响[J].大豆科学,2008,27(01):92-96.[doi:10.11861/j.issn.1000-9841.2008.01.0092]
　SUN Qing-yuan,ZHANG Xue-song,ZHAO Lue,et al.Effect of Urease Inhibitor NBPT on Growth of Soybean Roots[J].Soybean Science,2008,27(01):92-96.[doi:10.11861/j.issn.1000-9841.2008.01.0092]
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尿素酶抑制剂NBPT对大豆根系的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第27卷 期数: 2008年01期 页码: 92-96 栏目: 出版日期: 2008-02-25
Title:
Effect of Urease Inhibitor NBPT on Growth of Soybean Roots
作者:
孙庆元1; 张雪崧2; 赵略1; 刘娜1
1大连工业大学生物与食品工程学院，辽宁大连 116034；
2大连工业大学化工与材料工程学院，辽宁大连 116034
Author(s):
SUN Qing-yuan1; ZHANG Xue-song2; ZHAO Lue1; and LIU Na1
1 College of Bio & Food Technology，Dalian Polytechnic University，Dalian 116034，Liaoning；
2College of Chemical Engineering and Material，Dalian Polytechnic University，Dalian 116034，Liaoning，China
关键词:
尿素; NBPT; 大豆根系; 大豆根瘤
Keywords:
Urease; NBPT; Soybean root growth; Soybean root nodule
DOI:
10.11861/j.issn.1000-9841.2008.01.0092
文献标志码:
A
摘要:
大豆根系特别是与大豆共生的根瘤菌对土壤中的NH+4非常敏感，尿素酶抑制剂NBPT能够抑制土壤尿素酶的活性并减缓NH+4的水解速率已得到广泛的认同。为探索利用NBPT提高大豆生长发育的途径，对施用NBPT的大豆，利用氧化还原法，乙炔还原法和凯氏定氮法分别测定了大豆根系活性，根瘤固氮活性和植株含氮量，并测定了大豆根瘤的数量和重量。结果表明，NBPT提高了大豆整个生长期的根系活力，对根瘤固氮酶活性有促进作用，根瘤数量和重量都有增加，因而大豆植株的含氮量也随之增加，根系生长得到促进。NBPT作为氮素由于施用量小对大豆根系及根瘤的作用有限。
Abstract:
Soybean roots and particular the symbiosis root nodules are vulnerable to the NH+4?and NH3?in the soil.NBPT as soil urease inhibitor has been known to inhibit the urease activity in the soil and therefore deduce the hydrolysis velocity of urea.The purpose of this study was to find a way to increase soybean growth using NBPT.In this study，NBPT mixed proportionally with urea and NBPT without urea were applied to the soybean plants to analyze the effect of NBPT on the soybean root growth in root vigor，root nodule number and weight，and the activity of nitrogen fixation of root nodule.Results showed that NBPT mixed proportionally with urea increased the soybean root vigor，root nodule number and weight，the root nitrogen (N) fixation activity，and therefore the root growth was increased by a high content of N in the soybean plant.NBPT as a fertilizer applied was beneficial to soybean root growth.However，the effect of NBPT used as a fertilizer for N was limited.

参考文献/References:

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备注/Memo

基金项目：辽宁省教育厅科研项目(2004D203)；大连市重点科研项目(大连计发\[2003\]99号)；辽宁省发酵工程重点实验开放课题(辽发2006-009

)作者简介：孙庆元（1957-），男，博士，教授，从事尿素酶抑制剂应用及其作用机理研究。Tel：0411-86323740； E-mail：qsun@dlpu.edu.cn
通讯作者(Corresponding author)：张雪崧。Tel：0411-86323608; E-mail: zxs263@163.com

更新日期/Last Update: 2014-10-09