[1]莫贱友,郭堂勋,Navi S S,等.大豆叶部病害研究的简易离体叶片培养技术[J].大豆科学,2007,26(05):740-743.[doi:10.3969/j.issn.1000-9841.2007.05.021]
　MO Jian-you,GUO Tang-xun,Navi S S,et al.A SIMPLE DETACHED LEAF CULTURE TECHNIQUE FOR STUDY OF SOYBEAN FOLIAR DISEASE[J].Soybean Science,2007,26(05):740-743.[doi:10.3969/j.issn.1000-9841.2007.05.021]
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大豆叶部病害研究的简易离体叶片培养技术

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年05期 页码: 740-743 栏目: 出版日期: 2007-10-25
Title:
A SIMPLE DETACHED LEAF CULTURE TECHNIQUE FOR STUDY OF SOYBEAN FOLIAR DISEASE
文章编号:
1000-9841(2007)05-0740-04
作者:
莫贱友1; 郭堂勋1; 3; Navi S S2; 李讯2; 杨小冰2
1广西农业科学院植物保护研究所，南宁 530007；
2Department of plant pathology，Iowa State University，Ames，IA 50010；
3广西大学农学院，南宁 530004
Author(s):
MO Jian-you 1; GUO Tang-xun 1; 3; Navi S S2; LI Xun2; YANG Xiao-bing2
1Plant Protection Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007；
2Department of plant pathology，Iowa State University，Ames，Iowa 50010，USA；
3Agricultural College，Guangxi University，Nanning 530004
关键词:
大豆; 离体叶片; 再生根; 大豆锈病[Phakopsora pachyrhizi]; 大豆白粉病[Microsphaera diffusa]; 感病性
Keywords:
Soybean; Detach leaf; Root regeneration; Soybean rust(Phakopsora pachyrhizi Syd); Soybean powdery mildew (Microsphaera diffusa); Susceptibility
分类号:
S565.1
DOI:
10.3969/j.issn.1000-9841.2007.05.021
文献标志码:
A
摘要:
为寻找简易有效的大豆叶部病害研究方法，利用大豆离体叶片，进行了相关试验。试验结果表明，大豆离体复叶培养30 d，新根长达16.8～41.2 cm，平均根长＞24 cm，移至营养杯30 d后，叶色及叶片仍保持完好。经离体叶片法和整株法接种大豆锈病病原菌后，不同品种和不同菌株表达出不同的病斑反应型；接种大豆白粉病病原菌，也同样表达出在不同品种和不同接种浓度条件下，大豆白粉病发生程度不同；两种接种方试验结果一致。本方法试验条件可控、易控，省时，省空间，因此可考虑应用于大豆叶部病害研究的各种试验。
Abstract:
Detached leaves were used to find a simple and reliable method for studying the foliar diseases of soybean.The result showed the root generated from soybean detached leaf as long as 16.8~41.2 cm(with mean length of more than 24 cm)after culturing for 30 days，and remained green for additional 30 days in a pot with soil.It demonstrated that detached leaves produced identical or similar responses to leaves from the whole plants when inoculated with two fungal pathogens soybean rust(Phakopsora pachyrhizi Syd) and soybean powdery mildew(Microsphaera diffusa).This method could be applied for other foliar diseases study of soybean with easier controlled experiment condition and without more time and space.

参考文献/References:

［1］谈宇俊，孙永亮.大豆锈病病原菌生理小种的初步研究[J]大豆科学，1989，1:71-74..

[2]谈宇俊，孙永亮.大豆锈病的鉴定技术[J].中国油料，1989，1：65-67.
[3]Bromfield K R.Soybean Rust［C］.Monograph 11.The American Phytopathological Society，St.Paul，M N，1984.
[4]Burdon J J，Marshall D R.Evaluation of Australian native species of Glycine for resistance to soybean rust[J]Plant Disease，1981，65：44-45.
[5]Herath，I H M H B，Stoddard，F L，Marshall，D R.Evaluating faba beans for rust resistance using detached leaves[J].Euphytica.2001，117:47-57.
[6]Potential of detached soybean leaves for evaluation of rust resistance［C］.(Abst.) Poster presented at Molecular and Cellular Biology of the Soybean Conference in Lincoln，Nebraska，August 5-8，2006

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备注/Memo

基金项目：国家杰出青年科学基金“植物病害系统演变及长期动态”项目(30328018)提供部分资助

作者简介：莫贱友（1962－），男，副研究员，主要从事植物病害研究，E-mail: mojianyou@sina.com

更新日期/Last Update: 2014-10-20