[1]李文霞,李文滨,宁海龙,等.大豆花粉管通道法转化lkt50基因[J].大豆科学,2007,26(05):653-656.[doi:10.3969/j.issn.1000-9841.2007.05.003]
　LI Wen-xia,LI Wen-bin,NING Hai-long,et al.TRANSFORMING lkt50 GENE TO SOYBEAN BY POLLEN TUBE PATHWAY[J].Soybean Science,2007,26(05):653-656.[doi:10.3969/j.issn.1000-9841.2007.05.003]
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大豆花粉管通道法转化lkt50基因

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年05期 页码: 653-656 栏目: 出版日期: 2007-10-25
Title:
TRANSFORMING lkt50 GENE TO SOYBEAN BY POLLEN TUBE PATHWAY
文章编号:
1000-9841(2007)05-0653-04
作者:
李文霞1; 李文滨2; 宁海龙2; 吕文河1
1东北农业大学农学院，哈尔滨 150030；
2东北农业大学大豆研究所，国家教育部大豆生物学重点试验室，哈尔滨 150030
Author(s):
LI Wen-xia1; LI Wen-bin2; NING Hai-long2; Lv Wen-he1
1College of Agronomy，Northeast Agricultural University，Harbin 150030;
2Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology in Chinese Ministry of Education，Harbin 150030
关键词:
大豆; 花粉管通道法; lkt50
Keywords:
Soybean; Pollen-tube pathway; lkt50 gene
分类号:
S565.103.53
DOI:
10.3969/j.issn.1000-9841.2007.05.003
文献标志码:
A
摘要:
采用花粉管通道法，将外源基因lkt50向大豆品种东农48和品系东农96-02导入。通过卡那霉素筛选，从所获得的种子苗中初选转化植株，进一步进行PCR、Southern blot及RT-PCR分子生物学检测。结果表明：在14株卡那霉素抗性材料中利用nptII基因的特异性引物有7株PCR阳性，利用lkt-50基因特异性引物有2株PCR阳性，并且2株Southern blot及RT-PCR检测均呈现阳性，因此认为花粉管通道法可以用于大豆的转基因研究和应用。
Abstract:
lkt50 gene was introduced into soybean cultivar Dongnong 48 and strain Dongnong 96-02 via pollen tube pathway.The transformed plants were primary screened from seedling by spraying kanamycin，and further the transformed plants was determined by molecular biological methods including PCR，Southern blot and RT-PCR.The results indicated that the identification of 14 Km resistant plants was determined by PCR with the use of the primers of nptII and lkt50，and seven and two plants were found to be positive respectively.Furthermore，the detections by Southern blot and RT-PCR revealed that the two plants were found to be positive.The pollen tube pathway is an effective，feasible method for soybean transgene.

参考文献/References:

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备注/Memo

基金项目：黑龙江省科技攻关项目“大豆、马铃薯抗病和抗虫基因工程的研究”（GB04B101）

作者简介：李文霞（1974-），女，博士，讲师，研究方向为植物分子生物学。Tel:13796674549， E-mail:wxlee2006@yahoo.com.cn
通讯作者：李文滨教授，E-mail:wenbinli@yahoo.com；宁海龙副教授，E-mail:ninghailong1975@163.com ；吕文河教授，E-mail:whlu@mail.neau.edu.cn

更新日期/Last Update: 2014-10-20