[1]柳玉,郭顺堂.微生物在大豆籽粒中的分布及其对豆制品加工的影响[J].大豆科学,2007,26(04):578-582.[doi:10.3969/j.issn.1000-9841.2007.04.024]
　LIU Yu,GUO Shun-tang.DISTRIBUTION OF MICROORGANISM IN SOYBEAN GRAIN AND ITS EFFECTS ON THE PROCESS OF SOYBEAN PRODUCTS[J].Soybean Science,2007,26(04):578-582.[doi:10.3969/j.issn.1000-9841.2007.04.024]
点击复制

微生物在大豆籽粒中的分布及其对豆制品加工的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第26卷 期数: 2007年04期 页码: 578-582 栏目: 出版日期: 2007-08-25
Title:
DISTRIBUTION OF MICROORGANISM IN SOYBEAN GRAIN AND ITS EFFECTS ON THE PROCESS OF SOYBEAN PRODUCTS
文章编号:
1000-9841(2007)04-0578-05
作者:
柳玉; 郭顺堂
中国农业大学食品科学与营养工程学院，北京 100083
Author(s):
LIU Yu; GUO Shun-tang
Food Science and Nutritional Engineering College of China Agricultural University,Beijing,100083
关键词:
大豆; 微生物; 分布; 腐败
Keywords:
Soybean; Microorganism; Distribution; Spoilage
分类号:
TS214.2
DOI:
10.3969/j.issn.1000-9841.2007.04.024
文献标志码:
A
摘要:
针对豆制品实际加工过程中大豆中微生物洗不掉、除不去的问题进行了分析，采用表面消毒法和减压辅助消毒后大豆中细菌的检出率和细菌总数的测定证明了细菌主要附着于大豆籽粒最外层的柱状细胞以及外皮的气孔内部，而大豆子叶内部未检测到细菌的存在；并参照实际生产中的水洗和脱皮进一步说明了细菌与大豆柱状细胞的结合力较强且加工过程中易产生交叉污染，因此很难通过水洗和脱皮除去，最后通过组织分离培养法从大豆表皮中分离出了可能导致豆制品腐败的微生物并与豆浆中腐败菌进行了形态学方面的观察和比较，确定了原料中携带的造成豆制品腐败的主要微生物为芽孢杆菌。
Abstract:
Considering the problem that the microorganism could not been washed off during the industrial process of soybean products,this study focused on the distribution of microorganism in soybean grain.By using the surface and the vacuum degasification sterilization,measuring the soybean bacterial detection rate and the total bacterial numbers,it was demonstrated that the bacterial mainly attached on the outside surface of the soybean grain as well as the blowhole,at the same time the endophytic bacterial failed to be detected in the soybean cotyledon.After cleaning and dehulling according to the method of industrial process,it showed that the binding force of the microorganism with the soybean surface was apparent.And the study also revealed that the microorganism causing spoilage of soybean products is mainly the spore bacillus which exits in surface of the raw soybean

参考文献/References:

［1］谈志新.我国豆制品安全卫生质量评价[J].食品研究与开发，2002,23(4):79.

［2］渡边笃二.豆腐的科学[M].北京：中国食品工业协会豆制品专业委员会，2006:19,54.
［3］中国标准汇编.GB 2711-2003非发酵性豆制品及面筋卫生标准[S].北京：中国标准出版社，2003.
［4］项琦，万慕麟，周建新.粮油食品微生物学检验[M].北京：中国轻工业出版社，2001:123-125.
［5］中国标准汇编.GB 4789-2003.食品卫生检验方法[S].北京：中国标准出版社，2003:256-266.
［6］惠艺.原料奶中芽孢总数、耐热芽孢数的测定[J].中国乳品工业，1998,26(1):36.
［7］方中达.植物研究法（第3版）[M].北京：农业出版社，1990:123.
［8］牛天贵.食品微生物学实验技术[M].北京：中国农业大学出版社，2002:36-37.
［9］东秀珠，蔡妙英.常见细菌系统鉴定手册[M].北京：科学出版社，2001:205-207.
［10］赵斌，何绍江.微生物学实验[M]北京：科学出版社，2002:42-43.
［11］Kuklinsky-Sobral,J.Araujo,W.L.Mendes,R.et al.Isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion[J].Environmental Microbiology.2004,6(12):1244-1251.

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
[11]谷思玉,李欣洁,魏 丹,等.生物炭对大豆根际土壤养分含量及微生物数量的影响[J].大豆科学,2014,33(03):393.[doi:10.11861/j.issn.1000-9841.2014.03.0393]
　GU Si-yu,LI Xin-jie,WEI Dan,et al.Effects of Biochar on Soil Nutrient Contents and Microorganism of Soybean Rhizosphere[J].Soybean Science,2014,33(04):393.[doi:10.11861/j.issn.1000-9841.2014.03.0393]
[12]张红骥,孙彬,裴占江,等.有机无机复混肥对大豆根际微生物区系及幼苗生长的影响[J].大豆科学,2010,29(06):1016.[doi:10.11861/j.issn.1000-9841.2010.06.1016]
　ZHANG Hong-ji,SUN Bin,PEI Zhan-jiang,et al.Effects of Organic and Inorganic Compound Fertilizers on Soybean Rizosphere Microorganism and Seedling Growth[J].Soybean Science,2010,29(04):1016.[doi:10.11861/j.issn.1000-9841.2010.06.1016]

备注/Memo

基金项目：国家高技术研究发展计划（863计划）（2006AA102328）

作者简介：柳玉(1982-)，男，硕士研究生，研究方向为蛋白质加工与利用。
通讯作者：郭顺堂，教授，博士生导师。E-mail：shuntang@cau.edu.cn

更新日期/Last Update: 2014-10-20