[1]杨 柳 张彬彬 韩英鹏 　 李文滨.大豆亚麻酸含量的 QTL 分析*[J].大豆科学,2006,25(03):270-274,278.[doi:10.11861/j.issn.1000-9841.2016.03.0270]
　Yang Liu Zhang Binbin Han Yingpeng Li Wenbin.QTL ANALYSIS OF LINOLENIC ACID CONTENT IN SOYBEAN[J].Soybean Science,2006,25(03):270-274,278.[doi:10.11861/j.issn.1000-9841.2016.03.0270]
点击复制

大豆亚麻酸含量的 QTL 分析*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年03期 页码: 270-274,278 栏目: 出版日期: 2006-08-25
Title:
QTL ANALYSIS OF LINOLENIC ACID CONTENT IN SOYBEAN
作者:
杨 柳 张彬彬 韩英鹏 　 李文滨
(东北农业大学大豆研究所 ,教育部大豆生物学重点实验室,哈尔滨 150030)
Author(s):
Yang Liu　 Zhang Binbin　 Han Yingpeng 　 Li Wenbin
(Soybean Research Institute of NEAU ,Key Laboratory of Soybean Biology ,Ministry of Education , Harbin 150030)
关键词:
大豆; 亚麻酸含量; SSR 标记 ; QTL ; 遗传贡献率
Keywords:
Soybean ; Linolenic acid content ; SSR marker; QTL
DOI:
10.11861/j.issn.1000-9841.2016.03.0270
文献标志码:
A
摘要:
　 由于亚麻酸的高度不饱和性 ,导致大豆油的稳定性和耐贮性降低,并严重影响豆奶的商品价值。因此降低大豆种子亚麻酸含量是当今大豆育种的一个重要目标。本研究利用正常亚麻酸含量的品种合丰 25 与亚麻酸含量仅为 3%的突变品系 L - 14 杂交所得的 F 2 代和 F 3 代群体 ,对 500 多对 SSR引物进行筛选, 共有 90 个 SSR标记被分配到 1999 年 Cregan 定义的20条染色体中 , QTL分析表明有 3个分子标记 Satt066、Satt424 、Satt476 与大豆亚麻酸含量密切相关, 分别定位在连锁群 MLG B2 、MLG A2、MLG C1 上,且变异贡献率分别为 8. 7 % 、4. 99%和5. 3%。并用这些分子标记对 F 3 代家系进行了验证 。
Abstract:
Linolenic acid is the unstable component of soybean oil that is responsible for a shortened shelflife of the oil and commercial value of soybean milk . Therefore , the breeding objective to reduce linolenicacid levels in soybean oil is very important. In the present study , F 2 progeny segregating from a cross between Hefeng 25, a soybean cultivar with normal linolenic content , and L - 14 , a soybeanmutant line with only 3% linolenic acid, were used to screen over 500 SSR markers. A total of 90 SSRmarkers were distributed in twenty linkage groups developed by Cregan et al. (1999). QT L analysisshowed that three SSR markers Satt066、Satt424 、Satt476 were relevant to low linolenic acid, whih weredistributed in three linkage groups:MLG B2、MLG A2、MLG C1 ,respectively. The three QT Ls accountedfor 8. 7% 、4. 99%and 5. 3%of the phenotypic variation in linolenic acid level in this population , respec-tively. These markers have been tested on F 3 generation.

参考文献/References:

1　 王金陵, 杨庆凯, 吴宗璞. 中国东北大豆[ M] . 哈尔滨:黑龙江科学技术出版社, 1999:298 - 314.

2　 王金陵. 大豆[ M] . 哈尔滨:黑龙江科学技术出版社, 1982:1- 2.
3　 鲁振明. 大豆低亚麻酸种质的遗传与选育[ J] . 大豆通报, 1999, 4:16.
4　 马淑英, 梁歧. 大豆籽粒发育过程中脂肪酸的组分分析[ J] . 大豆科学, 1999, 18:124 - 128.
5　 朱军. 数量性状遗传分析的新方法及其在育种中的应用[ J] . 淅江大学学报(农业与生命科学版) , 2000, 26(1):1 - 6.
6　 胡明祥. 我国大豆品种脂肪酸组成的分析研究[ J] . 吉林农业科学. 1986,(1):12- 17.
7　 陈霞. 黑龙江省主栽大豆品种脂肪、脂肪酸组分的测定及其相关性的分析[ J] . 大豆科学, 1996, 15(1):91 - 95.
8　 裴东红. 降低大豆籽粒中亚麻酸含量的研究进展[ J] . 大豆科学,1995, 14(3):255 - 259.
9　 姚振纯. 大豆脂肪酸组分与改良[ J] . 大豆通报, 1997,(1):14.
10 刘丽君. 大豆亚麻酸的生化合成和调控[ J] . 大豆通报, 1993,(3):16 - 17.
11 S. M . Rahman, Y. Takagi . Inheritance of reduced linolenic acidcontent in soybean seed oil[ J] . T heor Appl Genet, 1997:299 -302.
12 I. Rajcan, K. J. Kasha , L. S. Kott1, et al. Detection of molecularmarkers associated with linolenic and erucic acid levels in springrapeseed [ M] . Kluwer Academic Publishers, 1999:173 - 181.
13 D. J. Somers , K. R. D. Friesen , G. Rakow. Identification ofmolecular markers associated with linoleic acid desaturation inBrassica napus[ J] . Theor Appl Genet, 1998, 96:897 - 903.
14 P. B. Cregan, T. Jarvik, A. L. Bush et al . An integrated ge-netic linkage map of the soybean genome[ J] . Crop Sci, 1999, 39:1464 - 1490.
15 Zenglu Li , Richard F , Wilson et al. Molecular mapping genes con-ditioning reduced palmitic acid content in N87- 2122 - 4 soybean[ J] . Crop Sci. , 2002, 42:373- 378.
16 Brummer, E. C. , A. D. Nickell, J. R. Wilcox , wt al. M appingthe Fan locus controlling linolenic acid content in soybean[ J] .Crop Sci. 1995, 34:829 - 833.
17 Keim, P. , T. C. Olson, R. C. Shoemaker. A rapid protocol for i-solating soybean DNA[ J] . Soy bean Genet. Newsl. 1988, 15:150- 152.
18 Zhu H, Hayes P , Kleinhofs A , Kudrna D, et al . Does functionfollow form principal QT Ls for fusarium head blight (FHB) re-sistance are coincident with Q TLs for inflorescence traits andplant height in a doubled-haploid population of barley[ J] . Theo-retical and Applied Genetics, 1999. 99(7- 8):1221 - 1232

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(03):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(03):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(03):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(03):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(03):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(03):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(03):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(03):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(03):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(03):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

备注/Memo

作者简介:杨柳(1981 -), 女, 硕士, 研究方向大豆生物技术。　

更新日期/Last Update: 2016-12-15