[1]王晓春,刘尚前王 　 罡季 　 静王 　 萍.大豆体细胞胚再生植株的研究＊[J].大豆科学,2006,25(02):149-152.[doi:10.11861/j.issn.1000-9841.2006.02.0149]
　Wang Xiaochun Liu Shangqian Wang Gang Ji Jing Wang Ping.A STUDY OF SOYBEAN PLANT REGENERATION VIA SOMATIC TISSUE CULTURES[J].Soybean Science,2006,25(02):149-152.[doi:10.11861/j.issn.1000-9841.2006.02.0149]
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大豆体细胞胚再生植株的研究＊

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年02期 页码: 149-152 栏目: 出版日期: 2016-05-25
Title:
A STUDY OF SOYBEAN PLANT REGENERATION VIA SOMATIC TISSUE CULTURES
作者:
王晓春1;  2　 刘尚前2王 　 罡1季 　 静1王 　 萍3
(1.天津大学农业与生物工程学院,天津 300072;2.河北北方学院南校区农业科学系, 河北宣化 075131;3.淮海工学院海洋学院 ,连云港 222005)
Author(s):
Wang Xiaochun1; 2　 Liu Shangqian2　 Wang Gang1　 Ji Jing1　 Wang Ping3
(1.Tian jin University of Agricultural and Biological Engineering Tianjin 300072;2.Department of Agriculture sciences of Hebei North University , X uanhua 075131;3.Huahai Institute of Technology , Marine School ,Lianyungang, 222005)
关键词:
大豆; 体细胞胚 ; 再生植株
Keywords:
Soybean ; Somatic embryos of soybean ; Regeneration plants
DOI:
10.11861/j.issn.1000-9841.2006.02.0149
文献标志码:
A
摘要:
以球形期大豆体细胞胚为材料,对其进行继代增殖、萌发及再生植株的研究。结果表明 ,大豆体细胞胚每隔 15-20 天分割成 3mm 左右的小块在 MS 培养基附加 10 -20 mg/ L 2,4 -D 的固体培养基以及弱光条件下 ,可以继续增殖 ,继代增殖能力强 , 扩繁系数为 3n (n 为继代培养次数);大豆体细胞胚性组织先在含有活性碳的培养基上培养, 能提高其萌发率以及正常胚率 ,1 个月以后转移到无活性碳的培养基 ,又能提高其再生速度以及再生率 。
Abstract:
　 The system of tissue culture were studied about sparal somatic embryos of soybean, the media a-bout the subculture ,germination and regeneration were established.The result showed that the media forsomatic embryogenesis proliferation was MS with 10～ 20mg/ L 2 ,4-D and weak light when they were di-vided into part somatic embryos with dismater 3mm every 15～ 20 day.The subculture coefficiency reached3 n ,("n"was the times of proliferation culture ),the germination frequency and normal somatic embryos fre-quency of soybean increased when active carbon was applied;when they were cultured one month underthis condition, again they were transferred into the media without active carbon, the regeneration frequencyand the regeneration speed of soybean increased.

参考文献/References:

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备注/Memo

项目来源:本研究由“国家植物转基因技术研究开发与中试基地建设”专项课题(J99-B-001)资助　?

作者简介:王晓春(1972-), 女, 讲师, 硕士, 主要从事作物栽培与遗传育种工作与研究。　?
通讯作者:王罡, 男, 教授, 博士生导师。Wgtt2003@y ahoo .com .cn

更新日期/Last Update: 2016-12-14