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微信公众号：大豆科学

[1]王　丽　宋志峰纪　锋　金卫东黄　璜　于志晶　唐晓博.高效液相色谱法测定大豆中的维生素 E 含量及其与粗脂肪含量的线性回归分析*[J].大豆科学,2006,25(02):113-117.[doi:10.11861/j.issn.1000-9841.2006.02.0113]
　Wang Li Song Zhifeng Ji Feng Jin Weidong Huang Huang Yu Zhijing Tang Xiaobo.THE DETERMINATION OF VITAMIN E USING HPLC AND THE LINEAR REGRESSION OFTHE CONTENTS OF VITAMIN E AND OIL IN SOYBEAN[J].Soybean Science,2006,25(02):113-117.[doi:10.11861/j.issn.1000-9841.2006.02.0113]

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高效液相色谱法测定大豆中的维生素 E 含量及其与粗脂肪含量的线性回归分析*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷期数: 2006年02期页码: 113-117 栏目: 出版日期: 2016-05-25

Title:: THE DETERMINATION OF VITAMIN E USING HPLC AND THE LINEAR REGRESSION OFTHE CONTENTS OF VITAMIN E AND OIL IN SOYBEAN

作者:: 王　丽 ¹　宋志峰 ¹ 纪　锋¹ 　金卫东 ¹ 黄　璜 ¹ 　于志晶 ² 　唐晓博 ³; (1. 吉林省农业科学院大豆研究中心, 公主岭 136100 ;2. 吉林农业大学研究生院,长春 130118 ;3. 吉林市农业科学院 , 长春 132101)

Author(s):: Wang Li ¹　 Song Zhifeng¹　 Ji Feng ¹ 　 Jin Weidong ¹　 Huang Huang ¹Yu Zhijing ²　 Tang Xiaobo ³; (1. Soybean Research Center of J ilin Academy of Agricultural Sciences ,Gongzhuling 136100;2. Graduate School of Jilin Agricultural University , Changchun 130118;3. Tilin City Academy of Agricultyral Science , Charychun 132101)

关键词:: 高效液相色谱法 ; 大豆; 维生素 E; 粗脂肪 ; 线性回归分析

Keywords:: HPLC ; Soybean ; Vitamin E ; Oil ; Linear regression

DOI:: 10.11861/j.issn.1000-9841.2006.02.0113

文献标志码:: A

摘要:: 利用高效液相色谱法测定 1000份大豆资源中的维生素 E 含量, 使用荧光检测器检测(Ex =295nm , Em =330nm),以 α- 、β - 、γ- 、δ- 生育酚为标准品 ,加入母育酚为内标, 计算大豆中维生素 E 各异构体的含量及总含量,α- 、β- 、γ- 、δ- 生育酚的加标回收率分别为 101. 10 %、97. 50%、96. 94%和 96. 30 %,测得野生大豆资源中维生素 E 含量范围 0. 05 ～ 0. 34mg /g ,栽培资源 0. 12～0. 38mg /g 。同时按国标方法测定粗脂肪含量 , 其中野生资源粗脂肪含量范围 3. 01 %～ 20. 60%,栽培资源 12. 00%～ 23. 59%。通过线性回归分析,明确了大豆中维生素 E 与粗脂肪含量呈正相关的线性关系。

Abstract:: T he contents of the vitamin E in 1000 soybean occessions were determined by high performanceliquid chromatography with fluorescence detection (Ex =295nm , Em =330nm). The isomers of vitamin Ewere determined by the standards α-、β-、γ-、δ-tocopherols and the internal standard of the tocol. The vita-min E contents was 0. 05～ 0. 34g /mg in wild soybean resources, 0. 10～ 0. 38g /mg in cultivated soybean re-sources, respectively. The average recoveries of α-、β-、γ-、δ-tocopherol were 101. 10 %, 97. 50%, 96. 94%and 96. 30%, respectively. Meanwhile , the oil contents of soybean resources were determined by nationalstandard method (GB 2906～ 82). Their range were 3. 01%～ 20. 60%in wild soybean resources,8. 00 %～23. 59%in cultivated soybean resources, respectively. The positive correlation between the contents of vi-tamin E and oil was confirmed by the analysis of linear regression.

参考文献/References:

1　郑云兰, 李霞辉. 大豆营养分析技术[ M] . 哈尔滨:黑龙江科学技术出版社, 1991, 190

2　崔洪斌. 大豆生物活性物质的开发与应用[ M] . 北京:中国轻工业出版社, 2001, 265 - 272

3　李里特. 大豆加工与利用[ M] . 北京:化学工业出版社, 2003, 38

4　中华人民共和国国家标准 - 谷类、油料作物种子粗脂肪测定方法GB2906 - 82[ S] . 北京:技术标准出版社, 1982

5 　 G. Panfili, P. M anzi , L. Pizzoferrato. High-performance liquidchromatog raphic method for the simultaneous determination of to-copherols, carotenes , and retinol and its geometric isomers in I-talian Cheeses[ J] . Analyst , No. 7, 1994(119):1161- 1165

6　 J. Lee , K. Suknark, Y. Kluvitse , et al. Rapid liquid chromato-g raphic assay of vitamin E and retinyl palmitate in extruded wea-ning foods[ J] . Journal of Food Science, No. 6, 1999(64):968- 972

7　于世林. 高效液相色谱方法及应用[ M] . 北京:化学工业出版社,2000, 243

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备注/Memo

?项目来源:美国伊利诺斯州瑞利生公司资助项目

?作者简介:王丽(1956- ), 女, 副研究员, 主要从事谷物品质分析研究。

通讯作者:宋志峰, T el:0434 - 6283231 E- mail:szf @cjaas. com

更新日期/Last Update: 2016-12-12