[1]乞永艳 　 Arnaud Bovy 　 唐益雄.外源茉莉酸对大豆异黄酮的影响*[J].大豆科学,2006,25(01):87-90.[doi:10.11861/j.issn.1000-9841.2006.01.0087]
　Qi Yongyan Arnaud Bovy T ang Yixiong.EFFECTS OF EXOGENOUS JASMONIC ACID ON ISOFLAVONOIDSIN Glycine max [ L.] MERRI.[J].Soybean Science,2006,25(01):87-90.[doi:10.11861/j.issn.1000-9841.2006.01.0087]
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外源茉莉酸对大豆异黄酮的影响*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第25卷 期数: 2006年01期 页码: 87-90 栏目: 出版日期: 2006-02-25
Title:
EFFECTS OF EXOGENOUS JASMONIC ACID ON ISOFLAVONOIDSIN Glycine max [ L.] MERRI.
作者:
乞永艳 1 　 Arnaud Bovy 2 　 唐益雄 3
(1. 中国农业科学院研究生院,北京 100081;2.Plant Research International , P. O. Box 16,6700 AA ,Wageningen , The Netherlands ;3. 中国农业科学院生物技术研究所 ,北京 100081)
Author(s):
Qi Yongyan1　 Arnaud Bovy2　 T ang Yixiong3
(1.Graduate School of Chinese Academy of Agricultural Sciences , Beijing, 100081 ;2. Plant ResearchInternational , P. O. Box 16, 6700 AA , Wageningen , The Netherlands ;3.Biotechnology Instituteof Chinese Academy of Agricultural Sciences, Beijing , 100081)
关键词:
大豆; 子叶 ; 茉莉酸; 异黄酮
Keywords:
Glycine max [ L. ] Merri ; Cotyledons; Jasmonic acid; Isoflavonoids
DOI:
10.11861/j.issn.1000-9841.2006.01.0087
文献标志码:
A
摘要:
摘下生长 8天的大豆子叶, 在不同浓度茉莉酸溶液中浸泡一定时间 ,用甲醇提取大豆异黄酮,经高压液相色谱(HPLC)测定,研究子叶表面伤口、浸泡时间以及茉莉酸浓度对大豆异黄酮的影响 。结果显示 :在对照组(0. 1%乙醇),子叶表面的伤口不影响异黄酮的分配比例 ;而用茉莉酸浸泡组 ,健康子叶中几乎不存在游离型的大豆甙元和染料木黄酮;丙二酰大豆甙和丙二酰染料木甙所占比例随着浸泡时间的延长而下降 ,大豆甙元和染料木黄酮所占比例随浸泡时间的延长而增加,大豆甙和染料木甙所占比例不随浸泡时间而变化;20μM 茉莉酸溶液浸泡的子叶中异黄酮总量最低 。
Abstract:
A series of experiments were designed to study the relationship between the isoflavonoids andthe cotyledon wounded surfaces, the concentrations of jasmonic acid (JA) and the soaked time. 8 - day -old soybean (Glycine max [ L. ] Merri)cotyledons were soaked into jasmonic acid solutions. Isoflavonoidswere extracted by methanol and detected by high pressure liquid chromatography(HPLC). T he resultsshowed that there was no effect the surface wound on the isoflavonoids. The ratio of the 6″- O - malonyl-daidzin(MGD) and 6″- O - malonylgenistin(MGG)decreased and the ratio of daidzein and genistein in-creased, while the ratio of daidzin and genistin kept stable during 72 hours soaked period. The total a-mount of isoflavonoids in 20μM JA treated samples was the lowest and decreased most quickly among the 5concentrations used.

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备注/Memo

基金项目:荷兰瓦赫宁根大学“交叉研究和教育基金” 项目, The Interdisciplinary Research and Education Fund (IN REF) (EstablishedBy the board of Wageningen University , the Netherlands)　?

作者简介:乞永艳(1970 - ), 女, 博士研究生, 研究方向:生物活性物质及其应用, E - mail:piaoliang - hao @163. com;Tel:010 -82111099, 13911879594, Fax :010- 88871902

更新日期/Last Update: 2016-12-12