Lang Man Liu Yuanying* Peng Xianlong Zhang Wenzhao.EFFECTS OF MAGNESIUM ON CARBON AND NITROGEN METABOLISMOF SOYBEAN AT DIFFERENT NITROGEN LEVELS[J].Soybean Science,2006,25(01):48-52,57.[doi:10.11861/j.issn.1000-9841.2006.01.0048]
不同氮肥用量下镁对大豆碳氮代谢的影响*
- Title:
- EFFECTS OF MAGNESIUM ON CARBON AND NITROGEN METABOLISMOF SOYBEAN AT DIFFERENT NITROGEN LEVELS
- Keywords:
- Soybean ; Magnesium(Mg); Carbon and nitrogen metabolism
- 文献标志码:
- A
- 摘要:
- 试验采用框栽法,在 N50kg /hm 2 ,N75kg /hm 2 两个水平下施用 MgO15kg /hm 2 , 测定了大豆叶片可溶性蛋白 、含氮量 、可溶性糖 、淀粉 、植株酰脲以及产量和籽粒蛋白含量 。结果表明, 与 N50相比 ,N50+Mg 的产量增加了 12. 95%,但差异不显著 ;与 N75 相比,N75+Mg 的各指标在各时期都有所增加 ,并达到了差异显著水平。其中可溶性蛋白含量在 V 4 和 R 2 期分别增加了 68. 04%和52. 07%;酰脲含量在 R 2 、R 4 、R 6 期分别增加了 75. 47%、21. 04%和 46. 39 %;淀粉含量在 R 4 、R 6 期分别增加了 59. 34%和 99. 02 %;产量和籽粒蛋白含量分别增加了 28. 67%和 3. 43%。与 N50 相比,N75的可溶性蛋白含量在 V 4 、R 2 、R 4 期分别增加了 36. 67%、65. 90%和 19. 70%,但酰脲含量在生殖生长期降低, 淀粉含量在 R 4 、R 6 期分别降低了 22. 34 %和 18. 21%;而 N75 +Mg 的酰脲含量比 N50 在 R 2 、R 4 、R 6 期分别增加了 59. 81%、8. 47 %和 25. 67%,淀粉含量在 R 4 、R 6 期也分别增加了 23. 60%和 62. 78%,差异显著 ,产量和籽粒蛋白含量各增加了 34. 38%,2. 93 %,均达到了 5%的显著水平 。这些结果表明, 在较高氮肥用量下施用适量的镁可协调碳氮代谢平衡 ,促进光合产物向碳氮方向合理分配 ,实现优质高产。
- Abstract:
- Pot experiment was conducted to determine the soluble protein , total nitrogen, soluble sugar,starch content of soybean leaves, ureide content of plant , soybean yield and seeds protein content when ap-plicating 15 kg /hm2MgO at N50 and N75 kg /hm2 . The results showed that compared with that of N50,the yield of N50+Mg increased by 12. 95%, but not significant. All the indexes contents of N75+Mg in-creased at different growth stage, and significant ,compared with that of N75. N75+Mg increased the sol-uble protein content by 68. 04 %and 52. 07%respectively at V 4 and R 2 stages, increased the ureide contentby 75. 47%, 21. 04% and 46. 39 % respectively at R 2 , R 4 , R 6 stages, increased the starch content by59. 34%and 99. 02% respectively at R 4 and R 6 stages, the yield and seeds protein content increased by 28. 67%and 3. 43%respectively. Compared with that of N50, the soluble protein content ofN75 increased by 36. 67%, 65. 90%, 19. 70%respectively at V 4 , R 2 , R 4 stages , significant at p=5%, butthe ureide content decreased at reproductive stage, and the starch content decreased by 22. 34% and 18.21% respectively at R 4 and R 6 stage, significant at p =5%. However , compared with that of N50, the ure-ide content of N75+Mg increased by 59. 81 %, 8. 47 %and 25. 67% respectively at R 2 , R 4 , R 6 stage , thestarch content increased by 23. 60%, 62. 78 %respectively at R 4 and R 6 stage , significant at p =5%, theyield and seeds content increased by 34. 38% and 2. 93% respectively , all significant at p =5%, whichdemonstrated that application of appropriate Mg at high nitrogen level could adjust the carbon and nitrogenmetabolism to realize high yield and good quality.
参考文献/References:
1 Walker C J, Werinstein JD. Further characterization of the mag-nesium chelatase in isolated developing cucumber chloroplasts[ J] . Plant Physiol, 1991, 95:1189- 1196.
相似文献/References:
[1]刘章雄,李卫东,孙石,等.1983~2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
备注/Memo
基金项目:国家“十五”重大科技攻关项目(项目编号:2001BA507A05 - 01)黑龙江省科技攻关项目(项目编号:GB04B717- 05)