Liu Lijun.THREE TYPES OF GENETIC MARKERS ASSOCIATED WITH RESISTANCE TO SOYBEAN SMV[J].Soybean Science,2005,24(04):310-313.[doi:10.11861/j.issn.1000-9841.2005.04.0310]
大豆花叶病毒遗传的三体标记＊
- Title:
- THREE TYPES OF GENETIC MARKERS ASSOCIATED WITH RESISTANCE TO SOYBEAN SMV
- Keywords:
- Soybean ; SMV ; Three types of genetic markers
- 文献标志码:
- A
- 摘要:
- 从大豆花叶病毒的形态抗性遗传标记 、分子生物学特点、生化遗传 、分子标记四个方面阐述了大豆研究所在大豆花叶病毒病方面所做的研究工作 ,以病毒病侵染大豆品种后的蛋白亚基变化、防卫系统细胞保护酶 -过氧化物酶 、苹果酸脱氢酶同工酶的酶蛋白分子表达特征,大豆叶肉细胞膜保护酶(超氧化物歧化酶 ,过氧化氢酶等)体系的变化特证;大豆细胞膜质过氧化有毒代谢产物的积累和变化, 以及 SMV 蛋白酶的分析结果 ,阐明了大豆病毒病的分子生物学特点;通过杂交和回交后代表型遗传特征鉴定和分析 ,证实大豆花叶病毒 SMV 的成株抗性的遗传基因表达特点, 并利用过氧化物酶和酯酶同工酶标记不同抗性品种和杂种后代的抗性遗传, 发现大豆病毒的生化遗传标记特征与成株抗性的表型特征相一致,可利用同工酶表达的遗传特征 ,筛选抗病毒杂交后代的一种技术 。同时利用 PCR 技术, 对杂种后代的 F 1 、F 2 代群体的抗性和遗传进行分析 , 证明大豆对SMV1 号株系的抗性是由一对显性基因控制的, 通过 RAPD 技术研究证实, 其显性 RAPD 标记是OPN 1400/ 1300 与黑农 39 的抗病基因的遗传距离为 8.2cm ,它为抗大豆花叶病遗传育种提供依据 。
- Abstract:
- The paper summarized that Soybean Research Institute Heilongjiang Academy of AgriculturalSciences had done some research on SMV from the aspects of morphology genetic marker , molecular biolo-gy characters, biochemical genetic and molecular genetic marker of SMV .It illustrated the molecular biol-ogy characters of SMV through analyzing the change of soybean protein ethyl of soybean cultivars inocula-ted with SMV1, the molecule expression characters of protective enzyme of defense system cell -peroxi-dase , malic dehydrogenase isoenzyme , the changeable characters of soybean protective enzymes system tomesophyll cells membrane , the deposition and change of soybean memeberanous peroxidatic toxiferous me-tabolite , and the results analyzing to SMV protein enzyme .Through appraising and analyzing inheritancephenotype of hybridization and phenotype of backcross mating , it testified that the genetic expressionalcharacters of SMV adult plant resistance .It used peroxidase and malic dehydrogenase isoenzyme in labelingresistance inheritance character of different resistant cultivars and their hybridization progenies and foundthat SMV biochemical genetic markers were in concordance with phenotype characters of adult plant resist-ance , and used isoenzyme expressional characters to filtrate the progenies resisting to virus.Using PCR toanalyze the F1, F2 group resistance and resistance inheritance , it testified that the resistance to SMV wasdecided by a couple of hypomorph .Through applying RAPD, it testified that the dominant RAPD labelingis OPN 1400/ 1300 and Heinong 39 resistant gene is 8.2cm , it will provide reference for this research in the fu-ture .
参考文献/References:
1 И.В.Кrishnan.Genetic engineering of soybean for improved nu-tritive value[ D] .Ⅳ International Soybean Processing and Utiliza-tion Conference, 2004, 388.
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备注/Memo
基金项目:国家 863 项目(2002AA211051-2;2002AA207007);黑龙江省自然科学基金、国家“948”项目