[1]乞永艳 　Arnaud Bovy Ric de Vos　唐益雄.外源茉莉酸对大豆中异戊烯黄酮 Glyceollins 及其前体 Glycinol 累积的影响*[J].大豆科学,2005,24(04):269-274.[doi:10.11861/j.issn.1000-9841.2005.04.0269]
　Qi Yongyan Arnaud Bovy Ric de Vos Tang Yixiong.EFFECTS OF EXOGENOUS JASMONIC ACID ON THE ACCUMULATIONOF GLYCEOLLINS AND GLYCINOLLN GLYCINE MAX [ L.] MERRI.[J].Soybean Science,2005,24(04):269-274.[doi:10.11861/j.issn.1000-9841.2005.04.0269]
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外源茉莉酸对大豆中异戊烯黄酮 Glyceollins 及其前体 Glycinol 累积的影响*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年04期 页码: 269-274 栏目: 出版日期: 2005-11-25
Title:
EFFECTS OF EXOGENOUS JASMONIC ACID ON THE ACCUMULATIONOF GLYCEOLLINS AND GLYCINOLLN GLYCINE MAX [ L.] MERRI.
作者:
乞永艳 1 　Arnaud Bovy 2Ric de Vos2　唐益雄 3
(1. 中国农业科学院研究生院, 北京 100081;2.Plant Research International , P. O. Box 16, 6700 AA ,Wageningen, T he Netherlands;3. 中国农业科学院生物技术研究所, 北京 100081)
Author(s):
Qi Yongyan 1Arnaud Bovy 2Ric de Vos 2Tang Yixiong 3
(1.Graduate School of Chinese Academy of Agricultural Sciences, Beijing , 100081;2.Plant Research International , P. O. Box 16 , 6700 AA , Wageningen , the Netherlands ;3. Biotechnology Institute of Chinese Academy of Agricultural Sciences , Beijing , 100081)
关键词:
大豆; 子叶; 茉莉酸; Glyceollins ; Glycinol ; 高压液相色谱
Keywords:
Glycine max [ L. ] Merri ; Cotyledons; Jasmonic acid; Glyceollins; Glycinol; High press liq-uid chromatography (HPLC)
DOI:
10.11861/j.issn.1000-9841.2005.04.0269
文献标志码:
A
摘要:
\以中黄 13 号大豆种子为材料 ,取生长 8 天的健康子叶, 在其表面挖出约2 ×2mm 的伤口,将50μl 不同浓度的茉莉酸溶液和 1mM 的硝酸银溶液滴在伤口处 ,黑暗中(25℃)保温不同时间后取样,经甲醇提取, 用高压液相色谱(HPLC)测定其中异戊烯化异黄酮 glyceollins 及其前体 glycinol的累积情况 。结果显示, 健康子叶中没有 glyceollins 和 glycinol 的累积, 1mM 硝酸银比茉莉酸具有更强的诱导 glyceollins 和 glycinol 累积的能力 ,glyceollins 的累积与茉莉酸浓度呈负相关。在各种处理中, glyceollin Ⅰ 、Ⅱ 、Ⅲ所占比例分别为:50%～ 80%、10%～ 40 %和 10%～ 20%, 但是glyceollin 三种异构体的比例随保温时间的延长而有所变化,glyceollin Ⅰ比例逐渐下降, glyceollinⅡ所占比例逐渐升高 ,在 1mM 硝酸银处理的子叶中表现更为明显。
Abstract:
Health cotyledons were detached from 8 - day - old soybean seedlings (Zhonghuang No. 13). Asection of tissue , approximately 2×2mm was cut from the concave surface. 50μl jasmonic acid or silver ni-trate solution was dripped into the wound and incubation in the dark at 25℃. Glyceollins and glycinol wereextracted by methanol and analyzed by HPLC. The results show that there is no accumulation of glyceol-lins and glycinol in health cotyledons. Silver nitrate acts much stronger than jasmonic acid to induce the ac-cumulation of glyceollins and glycinol. The accumulation of glyceollins is in inverse proportion to the con-centration of jasmonic acid. The percent of 3 glyceollin isomer is 50 %～ 80%, 10%～ 40%and 10%～ 20%for glyceollin I, II and III, respectively. During the incubation, the ratio of glyceollin I decreases and thatof glyceollin II increases. It can be obviously observed in samples treated by silver nitrate.

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备注/Memo

项目资助:荷兰瓦赫宁根大学“交叉研究和教育基金”项目, 项目名称:植物雌激素- 有健康保护作用的植物次生代谢物─植物中黄酮异戊烯转移酶基因的分离。

作者简介:乞永艳(1970 - ), 女, 博士研究生, 研究方向生物活性物质及其应用, E - mail:piaoliang - hao @163. com;电话:010 -82111099, 13911879594, 传真:010- 88871902

更新日期/Last Update: 2017-01-10