[1]王桂玲 　 黄永芬.胆碱磷酸转移酶基因转化大豆的初步研究*[J].大豆科学,2005,24(02):150-153.[doi:10.11861/j.issn.1000-9841.2005.02.0153]
　Wang Guiling Huang Yongfen.THE RESEARCH ON TRANSFERINGCPT GENE INTO SOYBEAN[J].Soybean Science,2005,24(02):150-153.[doi:10.11861/j.issn.1000-9841.2005.02.0153]
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胆碱磷酸转移酶基因转化大豆的初步研究*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年02期 页码: 150-153 栏目: 出版日期: 2005-05-25
Title:
THE RESEARCH ON TRANSFERINGCPT GENE INTO SOYBEAN
作者:
王桂玲 　 黄永芬
(哈尔滨师范大学生命科学系 ,哈尔滨 150080)
Author(s):
Wang Guiling 　 Huang Yongfen
(Harbin Normal University , Harbin 150080)
关键词:
大豆; 抗寒 ; CPT 基因; 转基因植物
Keywords:
Soybean ; Cold resistance ; CPT gene ; Transgenic plants
DOI:
10.11861/j.issn.1000-9841.2005.02.0153
文献标志码:
A
摘要:
胆碱磷酸转移酶基因(cpt)是磷脂合成代谢途径上一个关键的酶基因, 其基因产物是磷脂酰胆碱(PC),PC 做为主要膜脂与膜的流动性和植物的抗寒性有密切关系。本实验用花粉管通道法将质粒 PRDH401(含 35S - 35S - AMV - cpt - NOST)导入五个黑龙江常见栽培大豆品种中, 对D 1 代植株进行卡那霉素抗性筛选 、低温筛选和 PCR检测,初步筛选到转化植株 。
Abstract:
Cold resistance in the low temperature - induced plants is closely related to the synthesis metab-olism path of phospholipids. Cholinephospho transferase gene is a key enzyme gene in the synthesis metab-olism path of phospholipids. Phosphotidy choline(PC)is the enzyme gene product of CPT gene and it is thekey component of the membrane. . It is closely related with the mobility of membrane and cold resistance.In this experiment , plasmid PRDH401 which carrying the 35S - 35S - AMV - cpt - NOST fusiongene , was transferred into 5 varieties of soybean by means of the pollen tube pathwa. We got the D 1 genera-tion plants in the next year. Through the selection of kanamycin and low temperature, several transformedseedlings were obtained. PCR of the transformed soybean DNA indicated the integration of cpt gene intothe transgenic soybean genome.

参考文献/References:

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备注/Memo

基金项目:黑龙江省教育厅项目(10511031)　 作者简介:王桂玲(1964 -), 女, 博士研究生, 从事植物基因工程工作。

更新日期/Last Update: 2017-01-08