[1]郑蔚虹,田成亮　吴俊江　刘丽君**　孙剑秋.东北地区不同大豆品种间的 RAPD 分析*[J].大豆科学,2005,24(02):130-134.[doi:10.11861/j.issn.1000-9841.2005.02.0130]
　Zheng WeihongTian ChengliangWu JunjiangLiu Liyun**Sun Jianqiu.STUDIES ON GENETIC RELATIONSHIPS AMONG NORTHEASTSOYBEAN CULTIVARS OF CHINA BY RAPD[J].Soybean Science,2005,24(02):130-134.[doi:10.11861/j.issn.1000-9841.2005.02.0130]
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东北地区不同大豆品种间的 RAPD 分析*

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年02期 页码: 130-134 栏目: 出版日期: 2005-05-25
Title:
STUDIES ON GENETIC RELATIONSHIPS AMONG NORTHEASTSOYBEAN CULTIVARS OF CHINA BY RAPD
作者:
郑蔚虹1;  3　田成亮3　吴俊江2　刘丽君2**　孙剑秋3
(1. 温州大学生命与环境科学学院, 温州 325027;2. 黑龙江省农业科学院大豆研究所,哈尔滨 150086 ;3. 齐齐哈尔大学生命科学与工程学院生物系 ,齐齐哈尔 161006)
Author(s):
Zheng Weihong1; 3　Tian Chengliang3　Wu Junjiang2　Liu Liyun2**　Sun Jianqiu3
(1. School of Li f e and Environmental Sciences , Wenzhou University , Wenzhou 325027;2.Soybean, Institute Heilongjiang Academy of Agricultural Science Harbin 150086;3.Collegeof Lif e Science and Technology , Qiqihar University , Qiqihar 161006)
关键词:
大豆; 亲缘关系; RAPD
Keywords:
Soybean ; Cultivars; Genetic relationships; RAPD
DOI:
10.11861/j.issn.1000-9841.2005.02.0130
文献标志码:
A
摘要:
实验采用 RAPD 技术对我国东北地区不同生态区的野生大豆和栽培的大豆品种材料进行了种间遗传关系分析 。9 个引物共扩增出 141个片段 ,其中同源片段 6 个 ,特异性片段 21 个, 多态性片段 114 个, 显示出东北地区栽培的大豆品种间具有良好的多态性。 通过 NTSYS 软件对RAPD 结果进行 UPGMA 聚类分析, 得到了各品种间的亲缘关系树状图 。所有类型的品种在大约0. 45 - 0. 80 的距离水平被聚在一起, 并于近 0. 57 处分成 5 簇 。其中野大豆 01 - 336 居群与栽培种黑农 40 单独成簇 ,与其它品种间的亲缘关系最远, 其它野生居群及栽培品种交叉排列 , 分成 8簇。同一簇内野大豆居群间的遗传关系较近 ,与栽培大豆品种间遗传距离较远 ;不同簇间的野生大豆居群亲缘关系较远 ,隔离性强于簇内野生大豆与栽培种 。黑农 40 与其它栽培品种亲缘关系较远;农家品种小金黄 11905 与白眉 103亲缘关系很近, 与栽培品种合丰 34、抗线 3、绥农 8 亲缘关系相对近 ;农家品种黄宝珠与元宝金和宝丰 7亲缘关系相对较近。
Abstract:
　The genetic relationships among 8 populations of Glycinne soja and 20 varieties of G. max fromnortheast of China were analyzed by RAPD method. 141 bands were amplified totally with 9 primers, inwhich 6 bands were homologous , 21 bands were unique and 114 bands were polymorphic respectively. Theresults showed that there were high polymorphic between G. soja andG. max in Northeast of China. Phy-logenetic tree of cultivars was obtained through UPGMA cluster analysis. All cultivars were clustered at0. 45 to 0. 80 distance level , 5 clusters were branched at 0. 57. Wild species(G. soja 01- 336)and cultivar(G. max Heinong 40)were clustered solely from other cultivars, and the rest were crossed and divided to8 clusters again. In the same cluster , the relationships of the wild species were closed and insulated signifi-cantly with cultivars. In the different clusters, the relationships of the wild species were far and the segre-gation was stronger than G. soja and G. max that of the same cluster.

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备注/Memo

基金项目:国家高技术研究发展计划(863)编号 2002AA207007;黑龙江省教育厅科学技术研究项目, 编号 10541258

作者简介:郑蔚虹(1961 -), 女, 副教授, 研究方向植物生理与分子生物学。E - mail:zhengweihongl@sina. com
**通讯作者:E - mail:nky ssbd@mail. hl. cn

更新日期/Last Update: 2017-01-08