[1]叶小利 　 李学刚.蔗糖脂肪酸酯调节大豆叶片蔗糖酶生物合成机理研究[J].大豆科学,2005,24(02):95-100，102.[doi:10.11861/j.issn.1000-9841.2005.02.0095]
　Ye Xiaoli * Li Xuegang.Mechanism of Sucrose Fatty Acid Ester PromotingInvertase Biosynthesis in Soybean Leaves*[J].Soybean Science,2005,24(02):95-100，102.[doi:10.11861/j.issn.1000-9841.2005.02.0095]
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蔗糖脂肪酸酯调节大豆叶片蔗糖酶生物合成机理研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第24卷 期数: 2005年02期 页码: 95-100，102 栏目: 出版日期: 2005-05-25
Title:
Mechanism of Sucrose Fatty Acid Ester PromotingInvertase Biosynthesis in Soybean Leaves*
作者:
叶小利 1 　 李学刚 2
(1. 西南师范大学生命科学学院, 重庆 400715;2. 西南农业大学中心实验室 ,重庆 400716)
Author(s):
Ye Xiaoli1 *　 Li Xuegang2
(1.School of Life Science , Southwest Normal University , Chongqing , 400715 ;2.Central Laboratory , Southwest Agricultural University , Chongqing , 400716)
关键词:
大豆; 蔗糖酶; 蔗糖脂肪酸酯(SFE); AMD; CHI
Keywords:
Soybean ; Invertase ; SFE ; AMD; CHI
DOI:
10.11861/j.issn.1000-9841.2005.02.0095
文献标志码:
A
摘要:
2mmol /L 的 SFE 能显著地提高大豆蔗糖酶的活力 。SDS - PAGE 结果表明 SFE 主要通过增加大豆叶片蔗糖酶的生物合成量来大幅度地提高蔗糖酶的活力 。用转录抑制剂 AMD(8mg /L)和翻译抑制剂 CHI(1. 5mg /L)处理大豆叶片 ,发现它们都能在较短时间内大幅度地降低蔗糖酶的活性。SFE 对大豆开花期和结荚期的蔗糖酶生物合成量的增加是通过 SFE 促进蔗糖酶的转录水平和翻译水平来实现的, 转录起主要作用 。
Abstract:
Results showed that 2mmol /L of sucrose fatty acid ester (SFE) could increase the ac-tivity of invertase in soybean leaves, but no effects on the permeability of cell membrane includingthe leakage of K+and reducing glucose. The analysis of scanning on SDS - PAGE gels showedthat the increase of invertase activity after treated with 2mmol /L of SFE was caused by SFE en-hancing the content of increase. Experiments also indicated that 8mg /L of actinomycin D (AMD,a transcription inhibitor)and 1. 5mg /L of cycloheximede (CHI , a translation inhibitor)could de-cline the activity of invertase promoted by SFE in very short period obviously. It was suggestedthat SFE could increase invertase de novo biosynthesis on the level of the transcription mainly.

参考文献/References:

1　 Ye XL, Li XG , Cheng SH, et al . T he effects of surfactants onstomata and epicuticular wax structure of soybean[ J] . SoybeanScience , 2000, 19(1):49-53.

2　 Ye XL , Li XGg , Cheng SH , et al. The effects of sucrose fattyacid ester (SFE) on the activity of SOD and shlorophyll of soy-bean leaf[ J] . Soybean SCience, 2000, 19(4):330-336.
3　 Ye XL , Li XG, Cheng SH , et al. Effects of SFE on the activityand conformation of invertase of soybean leaf in vitro and in vivo[ J] . Acta Phytophysiologica Sinica, 2000, 26(5):446-452.
4　 Giaquinta , R. T. Sucrose translocation and storage in the glucosebeets[ J] . Plant Physiol. , 1979, 63:828～ 832.
5　 Dietmute EG , T homas R. Regulation and tissue - specific distri-bution of mRNAs for three extracellular invertase isoenzymes ofTomato suggests an important function in establishing and main-taining sink metabolism[ J] . Plant Phy siol, 1997, 115:273-282.
6　 Ricardo , C. P , Rees, T . Invertase activity during the developmentof carrot roots[ J] . Phytochemisty. 1970, 9:239-247.
7　 Roitsch T , Bittner M , Godt DE. Induction of apoplastic invertaseof Chenopodium rubrum by D - glucose and glucose analogue andtissue - specific expression suggest a role in sink - source regula-tion[ J] . Plant Physiol, 1995, 108:285-294.
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备注/Memo

　 基金项目:西南师范大学自然基金项目资助。


作者简介:叶小利(1971 -), 女, 博士, 副教授,从事天然药物化学方面的研究工作。

更新日期/Last Update: 2016-12-23