[1]刘北东朱延明李海燕杨谦.大豆子叶节再生影响因素的研究[J].大豆科学,2002,21(02):88-92.[doi:10.11861/j.issn.1000-9841.2002.02.0088]
　Liu Beidong ZhuYanmingLi Haiy anYang Qian.STUDYON FACTORS AFFECTING THE SOYBEAN COTYLEDONARY NODE REGENERATION[J].Soybean Science,2002,21(02):88-92.[doi:10.11861/j.issn.1000-9841.2002.02.0088]
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大豆子叶节再生影响因素的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第21卷 期数: 2002年02期 页码: 88-92 栏目: 出版日期: 2002-05-25
Title:
STUDYON FACTORS AFFECTING THE SOYBEAN COTYLEDONARY NODE REGENERATION
文章编号:
1000 -9841(2002)02 -0088 -05
作者:
刘北东1朱延明2李海燕2杨谦1
1.哈尔滨工业大学生命科学与工程系, 哈尔滨150001 ;
2.东北农业大学生命科学学院, 哈尔滨150030
Author(s):
Liu Beidong 1ZhuYanming2Li Haiy an2Yang Qian1
1.Department of Li fe Science and Engineering , Harbin Inst itute of Technology , Harbin 150001 ;
2.Li fe Science Col lage , Northeast Agriculture University , Harbin 150030)
关键词:
大豆; 子叶节; 丛生芽; 超声波辅助处理
Keywords:
Soybean ; Coty ledonary node ; Multiple shoot ; Sonication assisted t reatment
分类号:
S 565.035.3
DOI:
10.11861/j.issn.1000-9841.2002.02.0088
文献标志码:
A
摘要:
为了建立一个大豆子叶节高效再生体系用于大豆的遗传转化, 选用10 个东北主栽大豆品种(Glycine max (L .)Merr .)的子叶节作为外植体, 研究了基因型、植物激素的浓度、超声波辅助处理时间...等6 种影响大豆子叶节再生的因素。结果表明合丰35 、合丰25 、黑农40 再生效率较高,平均每个外植体分化出的丛生芽数分别为6 .69 、6 .32 和5 .98 ;确定了丛生芽分化阶段所需的BA浓度和生根阶段所需的IBA 浓度, 发现不同基因型需要不同的BA 浓度, 合丰35 、合丰25 为1 .5mg/l , 黑农40 为1 .2mg/l 。适宜的外植体大小为保留全部子叶。作为遗传转化时的除菌剂, 头孢唑啉钠(Cef)在500mg/l 时对子叶节的再生无显著影响。当遗传转化时使用的超声波辅助处理时间小于12 秒时, 对子叶节再生无显著影响。
Abstract:
To establish a high frequency regeneration system for the gene t ransfo rmation of soybean(Glycinemax (L .)Merr .), cotyledonary nodes f rom ten varieties w ere used as the ex plants in the regenerat ionexperiments.Six factors such as geno types , plant grow th regulators , sonication assisted t reatment and so onw ere studied .The result s show that the high regeneration f requency geno types are Hefeng 35 , Hefeng 25 ,Heinong 40 , the multiple shoot s per explant are 6 .69 , 6 .32 and 5 .98 respect ively .The BA concentrationsrequired in shoot dif ferentiation medium are different among these geno types, in Hefeng 35 , 1 .5mg/l Hefeng 25and in 1 .2mg/l Heinong 40 .But the IBA concentrations in root regenerat ion medium are the same .The fullcotyledo nary is the best explant size for shoo t differentiation .As an antibiotic to restrain the propagatio n ofexcess Ag robacterium , the Cef has no sig nificant inf luence on regeneration .When the t reatment time is shorterthan 12s , the sonicat ion assisted t reatment has no sig nificant influence on the regeneration of shoots .

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备注/Memo

作者简介:刘北东(1972 -), 男, 博士研究生, 研究方向微生物与植物基因工程。

更新日期/Last Update: 2016-12-30