[1]韩玉琴　卢翠华　李希臣　周思军　刘昭军　雷勃均　钱华.辐照外源DNA 直接导入大豆诱变效果的研究[J].大豆科学,2001,20(04):254-256.[doi:10.11861/j.issn.1000-9841.2001.04.0254]
　Han Yuqin Lu Cuihua Li Xichen Zhou Sijun Liu Zhaojun Lei Bojun Qian Hua.STUDIES ON THEMUTATION EFFECTS INDUCED BY DIRECTINTRODUCTION OF IRRADIATED FOREIGN DNA INTO SOYBEAN[J].Soybean Science,2001,20(04):254-256.[doi:10.11861/j.issn.1000-9841.2001.04.0254]
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辐照外源DNA 直接导入大豆诱变效果的研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第20卷 期数: 2001年04期 页码: 254-256 栏目: 出版日期: 2001-11-25
Title:
STUDIES ON THEMUTATION EFFECTS INDUCED BY DIRECTINTRODUCTION OF IRRADIATED FOREIGN DNA INTO SOYBEAN
文章编号:
1000 -9841(2001)04 -0254 -03
作者:
韩玉琴　卢翠华　李希臣　周思军　刘昭军　雷勃均　钱华
黑龙江省农业科学院生物技术研究中心哈尔滨150086
Author(s):
Han Yuqin 　Lu Cuihua 　Li Xichen 　Zhou Sijun 　Liu Zhaojun 　Lei Bojun 　Qian Hua
Biotechnology Research Center , Heilongjiang Academy of Agricultural Sciences , Harbin 150086
关键词:
大豆; 外源DNA ; 辐照
Keywords:
Soybean ; Foreig n DNA ; Irradiation
分类号:
S651.103.52
DOI:
10.11861/j.issn.1000-9841.2001.04.0254
文献标志码:
A
摘要:
提取农作物总DNA , 采用软x 射线, 剂量为2Gy 、20Gy 、200Gy 和60C0γ射线, 剂量为1Gy 、3Gy 和5Gy 进行照射, 利用花粉管通道技术将其直接导入大豆。以导入不经照射的DNA 为对照,实验结果表明:导入受照射的DNA 明显的降低了D0 代的结实率和D1 代的成苗率, 但后代材料的变异率明显提高, 并且出现了供体和受体都不具备的新性状。
Abstract:
Foreign total DNA of crop was i rradiated w ith sof t x -ray in doses of 2Gy , 20Gy amd 200Gyrespertivelg and60Coγ-ray in dosages of 1Gy , 3Gy and 5Gy respectively , and was int roduced into soybeanthroug h pollen tube channel .The same foreign DNA w ithout i rradiation treatment also int roduced into thesoybean w as used as CK .the result s indicated , althong h the seed rate of D0 and the germination rate of D1 w erelower than its CK , the mutation rate of the later generatio ns was increased compared with CK .Moreover , somenew characters which were different from the supplier and the receptor w ere produced in the descendants .

参考文献/References:

1 　李忠杰, 孙光祖, 王广金, 等.辐照外源DNA 导入小麦诱变效果的研究[ J] .核农学通报, 1996 , 17(4):151 -154.

2 　卢翠华, 韩玉琴, 李希臣, 等.辐照外源DNA 导入大豆的研究初报[ J] .黑龙江农业科学, 2000 ,(2):17 -18.
3 　周光宇, 翁坚, 龚蓁蓁, 等.农业分子育种受粉后外源DNA 导入植物的技术[ J] .中国农业科学, 1988 , 21(3):1 -6.
4 　雷勃钧, 尹光初, 王树林, 等.外源野生大豆DNA 导入栽培大豆引起的变异[ J] .中国油料, 1989 ,(3):11 -13.
5 　李忠杰, 孙光祖, 王广金, 等.辐照外源DNA 导入小麦诱变效果初探[ J] .核农学通报, 1995 , 16(1):1 -4.

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备注/Memo

基金项目:黑龙江省自然科学基金资助项目。　

作者简介:韩玉琴(1956 -)女, 副研究员, 从事生物技术研究

更新日期/Last Update: 2016-12-29