[1]田清震盖钧镒喻德跃贾继增.大豆DN A扩增片段长度多态性( AFLP)研究[J].大豆科学,2000,19(03):210-217.[doi:10.11861/j.issn.1000-9841.2000.03.0210]
　Tian Qing zhenGai Junyi?Yu DeyueJia Ji zeng.A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM( AFLP) IN SOYBEAN[J].Soybean Science,2000,19(03):210-217.[doi:10.11861/j.issn.1000-9841.2000.03.0210]
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大豆DN A扩增片段长度多态性( AFLP)研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第19卷 期数: 2000年03期 页码: 210-217 栏目: 出版日期: 2000-08-25
Title:
A STUDY ON AMPLIFIED FRAGMENT LENGTH POLYMORPHISM( AFLP) IN SOYBEAN
作者:
田清震1盖钧镒1喻德跃1贾继增2
1. 南京农业大学大豆研究所农业部国家大豆改良中心,南京210095;
2.中国农科院品种资源研究所农业部作物品种资源重点实验室,北京100081
Author(s):
Tian Qing zhen1　Gai Junyi1?　Yu Deyue1　Jia Ji zeng2
1. Soybean Research Institute, Nanjing Agriculture University; National Center ofSoybean Improvement , Ministry of Agricul ture, Nanjing , 210095;?
2. Institute ofCrop Germplasm Resour ces , Chinese Academy of Agricul tural Science; KeyLaboratory of Crop Germplasm & Biotechnology , Ministr y of Agr iculture, Bei jing , 100081
关键词:
大豆;  DN A提取;  扩增片段长度多态性( AFLP) ;  银染检测
Keywords:
Soybean;  DNA ex traction;  Silv er - s taining inspection;  AFLP ( amplifiedf ragment leng th polymo rphism)National Key Science and Technology Pro ject No. 960140106Author f or correspondence
DOI:
10.11861/j.issn.1000-9841.2000.03.0210
文献标志码:
A
摘要:
本文研究AFLP技术在大豆上的应用,在改进大豆种子DN A提取方法的基础上,比较同位素与银染检测方法的效果,筛选适宜于大豆AFLP分析的酶切和引物组合,从而建立了适用于大豆指纹图谱快速鉴定的AFLP操作程序,并对我国野生大豆和栽培大豆代表性材料进行了AFLP分析。
Abstract:
In the present paper, improvement of the DN A ex traction f rom dry soybean s eed,comparison between the silv er s taining and isot ope labeling methods, and ex plora tion of theendoenzyme and primer combinations sui table f or AFLP analysis in soybeans were presented.Based on this , a simple and f as t procedure suitable fo r soybean AFL P analysis w asestablished, and the repres entativ e accessions of G. soja and G.max f rom v arious ecologicalareas in China were examined by using the sugges ted procedure.

参考文献/References:

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2　Keim, P. , R. C. Sh oemaker, and R. G. Palmer. Res t ricti on f ragm ent l eng th pol ymorphism diversit y in s oybean.Th eor. Appl. Genet. , 1989, 77: 786～ 792
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7　Vos, P, R. , Hog ers , M. Bl eek er, et al. AFLP: a new t ech nique f or DNA f ingerprin ting. Nuclei c Acids Res. ,1995, 23( 21): 4407～ 4414?
8　Zabeau M. and Vos P. Selectiv e res tricti on f ragm en t ampli fication: a g eneral meth od f or DNA fingerprinting.European Pat en t Applicati on num ber 92402629. 7; Publication number 0535858A1. Eu ropean Patent Off ice,Paris. 1993
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10　Maughan, P. J. , M. A. S Maroof, G. R. Bus s, et al. Ampli fi ed f rag men t length polym orph ism ( AFLP) insoybean: s peci es diversit y, inh eri tance, near- isogenic lin e analysi s. Th eor. Appl. Genet. , 1996, 93: 392～ 401
11 　Pow el l, W. , M. Mo rgant e, C. Andre, et al. Th e compari son of RFLP, RAPD, AFL P and S SR( micros atelli te) mark ers f or g ermpl asm analysi s. Molecular Breeding, 1996, 2: 225～ 238
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更新日期/Last Update: 2016-12-27