[1]南相日　刘文萍　刘丽艳　吕晓波　何云霞 卫志明.PEG介导BT基因转化大豆原生质体获转基因植株[J].大豆科学,1998,17(04):326-330.[doi:10.11861/j.issn.1000-9841.1998.04.0326]
　Nan Xiang riLiu WenpingLiu LiyanLu XiaoboHe Yunxia?Wei Zhiming.PEG- MEDIATED TRANSFORMATION AND REGENERATION OFSOYBEAN PROTOPLAST WITH B ACI LLUS T HURIN GIEN SI S CrgIAc GENE[J].Soybean Science,1998,17(04):326-330.[doi:10.11861/j.issn.1000-9841.1998.04.0326]
点击复制

PEG介导BT基因转化大豆原生质体获转基因植株

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第17卷 期数: 1998年04 页码: 326-330 栏目: 出版日期: 1998-11-20
Title:
PEG- MEDIATED TRANSFORMATION AND REGENERATION OFSOYBEAN PROTOPLAST WITH B ACI LLUS T HURIN GIEN SI S CrgIAc GENE
作者:
南相日　刘文萍　刘丽艳　吕晓波　何云霞 卫志明
(黑龙江省农业科学院生物技术研究中心,哈尔滨150086)
(中国科学院上海植物生理研究所,上海200032)
Author(s):
Nan Xiang ri　Liu Wenping　Liu Liyan　Lu Xiaobo　He Yunxia?Wei Zhiming
(B io. Res. Center , Heilongjiang Academy of Agri . Sci. Harbin , 150086)
( Shanghai Institute of Plant Physiology , Academia of Sinica , Shanghai , 200032)
关键词:
大豆;  B. T毒蛋白基因;  基因组
Keywords:
Soy bean;  Baci llus thuringiensis Cry IAc gene;  Genome
DOI:
10.11861/j.issn.1000-9841.1998.04.0326
文献标志码:
A
摘要:
通过PEG法将B. T ( Bacillas tharingiensis Cry IAc)毒蛋白基因导入到大豆主栽品种黑农35,黑农37,合丰25和合丰35的原生质体中,经30ml /L潮霉素筛选,选择有抗性的愈伤组织进行分化,获得了三棵再生植株,移栽后全部成活,对移栽后植株的总DN A进行PCR分析,均显阳性。对PCR阳性植株进行Southern杂交分析,证明B. T毒蛋白基因已整合到大豆细胞基因组中。
Abstract:
Baci llus thuringiensis Cry IAc g ene was int roduced into the pro to plast of leadingsoybean cul tiv ars Heino ng 35, Heino ng 37, Hefeng 25 a nd Hefeng 35 wi th the PEGmethod. Through the screening wi th 30mg /l hyg romycin and the di fferentia tion of selectedresista nt calli, three reg enerated plants w ere obtained and t ransplanted successfully. PCR analy sis of the DN A f rom the transplanted plant s show ed posi tiv e reactio n.Southern blo t ana lysis of the PCR- posi tiv e pla nt s prov ed that the B. T Cry IAc g enehad integ ra ted into the g enome of these pla nt s.

参考文献/References:

[1 ]　丁群星、谢友菊、戴景瑞等, 1993,中国科学, ( B辑) , 2( 7): 707- 713

[2 ]　卫志明、许智宏, 1990,大豆原生质体培养和植株再生,植物学报, 32(8) : 582- 588
[3 ]　卫志明、黄健秋、徐淑萍等, 1996,植物遗传国家重点实验室年报, 36- 37
[4 ]　傅荣昭、孙勇如、贾士荣主编,植物遗传转化技术手册,中国科学技术出版社
[5 ]　Chris tou, P. et al. , 1989, Inherit ance and expression of f oreig n g enes in t ransg enic soybean pl an ts. Proc.Nat . Acad. Sci. U SA, 86, 7500- 7504[6 ]　Deblock , M. et al. , 1984, Expression of f oreig n genes in regenerated plants and th ei r progency. EM BO J. ,3, 1681- 1689[7 ]　Finer J J. McMul len MD, 1991, Transf ormati on of s oybean via particle bombardmen t of embryogeni c s uspensi on culture tis sue. In Vi t ro Cell Dev Bi ol. 27, 175- 182[8 ]　Henddw yn, J. 1994, Plant s g ene t ran sf er and exp ressi on prot ocols , Meth ods in molecul ar bi ology 49, 161-179[9 ]　Hinch ee, M. A. W. , et al. , 1988, Producti on of transg enic soybean pl an ts using agrobacterium mediat edDN A t rans f er. Bi o / tech nology, 6, 915- 922[10 ]　Horsch , R. B. , et al. , 1984, In h eri tance of f ancti onal f oreig n genes in Plan ts. Science, 223, 496- 498
[11 ]　Mccab e, D. E. et al. , 1998, Stabes t ransf ormati on of s oybean ( Gl ycine Max ) by par ticl e accel ation. Bio /Techonl ogy, 6, 923- 926
[12 ]　Sarw an , K. Dhi r et al. , 1991, Plant let regenerati on f rom imm eture cotyl edon prot oplast s of s oybean( Gl ycine Max L. ) . Plant s Cell Rep. 10, 39- 43
[13 ]　Sat o, S. et al. , 1993, St ab e t rans f ormation Via par ticle bom bardm en t in tw o dif f erent soybean t ransf ormation sys tems. Plant Cell Rep. 12, 408- 413
[14 ]　St ew art CN Jr. Via LE 1993, A rapid CT AB DN A is olation t ech nique useful f or RAPD fingerp rinting andoth er PCR applicati ons. Bi ot ech niques , 14, 748- 751
[15 ]　St ew art CN. Jr. et al. , 1996, Gen eti c t ransf ormati on, Recov ery, and charact eri zat ion of f erti le Soybeant rans genic f or syn theti cB aci llus th uringi ensis Cr yI Ac Gene. Plant Ph ys iol. 112, 121- 129

相似文献/References:

[1]刘章雄,李卫东,孙石,等.1983～2010年北京大豆育成品种的亲本地理来源及其遗传贡献[J].大豆科学,2013,32(01):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
　LIU Zhang-xiong,LI Wei-dong,SUN Shi,et al.Geographical Sources of Germplasm and Their Nuclear Contribution to Soybean Cultivars Released during 1983 to 2010 in Beijing[J].Soybean Science,2013,32(04):1.[doi:10.3969/j.issn.1000-9841.2013.01.002]
[2]李彩云,余永亮,杨红旗,等.大豆脂质转运蛋白基因GmLTP3的特征分析[J].大豆科学,2013,32(01):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
　LI Cai-yun,YU Yong-liang,YANG Hong-qi,et al.Characteristics of a Lipid-transfer Protein Gene GmLTP3 in Glycine max[J].Soybean Science,2013,32(04):8.[doi:10.3969/j.issn.1000-9841.2013.01.003]
[3]王明霞,崔晓霞,薛晨晨,等.大豆耐盐基因GmHAL3a的克隆及RNAi载体的构建[J].大豆科学,2013,32(01):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
　WANG Ming-xia,CUI Xiao-xia,XUE Chen-chen,et al.Cloning of Halotolerance 3 Gene and Construction of Its RNAi Vector in Soybean (Glycine max)[J].Soybean Science,2013,32(04):12.[doi:10.3969/j.issn.1000-9841.2013.01.004]
[4]张春宝,李玉秋,彭宝,等.线粒体ISSR与SCAR标记鉴定大豆细胞质雄性不育系与保持系[J].大豆科学,2013,32(01):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
　ZHANG Chun-bao,LI Yu-qiu,PENG Bao,et al.Identification of Soybean Cytoplasmic Male Sterile Line and Maintainer Line with Mitochondrial ISSR and SCAR Markers[J].Soybean Science,2013,32(04):19.[doi:10.3969/j.issn.1000-9841.2013.01.005]
[5]卢清瑶,赵琳,李冬梅,等.RAV基因对拟南芥和大豆不定芽再生的影响[J].大豆科学,2013,32(01):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
　LU Qing-yao,ZHAO Lin,LI Dong-mei,et al.Effects of RAV gene on Shoot Regeneration of Arabidopsis and Soybean[J].Soybean Science,2013,32(04):23.[doi:10.3969/j.issn.1000-9841.2013.01.006]
[6]杜景红,刘丽君.大豆fad3c基因沉默载体的构建[J].大豆科学,2013,32(01):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
　DU Jing-hong,LIU Li-jun.Construction of fad3c Gene Silencing Vector in Soybean[J].Soybean Science,2013,32(04):28.[doi:10.3969/j.issn.1000-9841.2013.01.007]
[7]张力伟,樊颖伦,牛腾飞,等.大豆“冀黄13”突变体筛选及突变体库的建立[J].大豆科学,2013,32(01):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
　ZHANG Li-wei,FAN Ying-lun,NIU Teng-fei?,et al.Screening of Mutants and Construction of Mutant Population for Soybean Cultivar "Jihuang13”[J].Soybean Science,2013,32(04):33.[doi:10.3969/j.issn.1000-9841.2013.01.008]
[8]盖江南,张彬彬,吴瑶,等.大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究[J].大豆科学,2013,32(01):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
　GAI Jiang-nan,ZHANG Bin-bin,WU Yao,et al.Screening of Soybean Genotypes Suitable for Suspension Culture with Adventitious Embryos and Genetic Transformation by Particle Bombardment[J].Soybean Science,2013,32(04):38.[doi:10.3969/j.issn.1000-9841.2013.01.009]
[9]王鹏飞,刘丽君,唐晓飞,等.适于体细胞胚发生的大豆基因型筛选[J].大豆科学,2013,32(01):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
　WANG Peng-fei,LIU Li-jun,TANG Xiao-fei,et al.Screening of Soybean Genotypes Suitable for Somatic Embryogenesis[J].Soybean Science,2013,32(04):43.[doi:10.3969/j.issn.1000-9841.2013.01.010]
[10]刘德兴,年海,杨存义,等.耐酸铝大豆品种资源的筛选与鉴定[J].大豆科学,2013,32(01):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]
　LIU De-xing,NIAN Hai,YANG Cun-yi,et al.Screening and Identifying Soybean Germplasm Tolerant to Acid Aluminum[J].Soybean Science,2013,32(04):46.[doi:10.3969/j.issn.1000-9841.2013.01.011]

更新日期/Last Update: 2017-01-14