[1]张志永①②　陈受宜　　盖钧镒　胡蕴珠　智海剑.栽培大豆品种间RAPD标记的多态性分析及聚类分析[J].大豆科学,1998,17(01):1-9.[doi:10.11861/j.issn.1000-9841.1998.01.0001]
　Zhang Zhiyo ngChen Shouyi Gai JunyiHu YunzhuZhi Haijian.POLYMORPHISMS AND CLUSTER ANALYSIS OFCULTIVATED SOYBEANS WITH RAPD[J].Soybean Science,1998,17(01):1-9.[doi:10.11861/j.issn.1000-9841.1998.01.0001]
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栽培大豆品种间RAPD标记的多态性分析及聚类分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第17卷 期数: 1998年01 页码: 1-9 栏目: 出版日期: 1998-02-20
Title:
POLYMORPHISMS AND CLUSTER ANALYSIS OFCULTIVATED SOYBEANS WITH RAPD
作者:
张志永①②　陈受宜　　盖钧镒　胡蕴珠　智海剑　
(中国科学院遗传所①,北京100101)　　
(南京农业大学②,南京210095)
Author(s):
Zhang Zhiyo ng　Chen Shouyi Gai Junyi　Hu Yunzhu　Zhi Haijian
( Inst itute of Genetics, Academia Sinica , 100101)’
( Soybean Research Institute , Nanjing A gricul tural University , 210095)
关键词:
栽培大豆;  RAPD多态性;  聚类分析
Keywords:
Cultiva ted so ybeans;  RAPD;  Po lymo rphi sms;  Cluster analy sis
DOI:
10.11861/j.issn.1000-9841.1998.01.0001
文献标志码:
A
摘要:
应用从6000多份大豆种质资源中筛选出的21个抗大豆花叶病毒病的品种或品系及7个感病的品种或品系,选用20个随机引物,对总DN A进行了随机扩增。有18个引物扩增得到了稳定的RAPD图谱, O PH- 06和O PH- 10未能扩增到RAPD产物。扩增出的片段分子量在0. 6- 4. 6Kb之间。随机引物扩增出的条带数在5- 17条之间,共扩增出165个条带,品种间相同的条带有104个,多态性条带有61个,多态性条带占37%。这20个引物可分为①无扩增产物的引物; ②扩增产物无多态性的引物; ③扩增产物多态性低于15%的引物;④扩增产物多态性在15% - 30%之间的引物; ⑤扩增产物多态性在30% - 50%之间的引物; ⑥扩增产物多态性超过50%的引物。依据遗传距离进行聚类分析的结果表明,这些材料可以明确地聚成5组。各组内姊妹系衍生出的品种或品系首先聚在了一起,其次有一定血缘关系的聚在了亚组里。另外,各组内或亚组内的品种或品系在地理分布上具有一定相关性。
Abstract:
In present study , tw enty- one so ybean accessions wi th host resista nce to di fferentSMV st rains and 7 susceptible cul tiv ars or lines w ere a nalysed for their g enetic diversi tyusing RAPD. Tw enty 10- mer random primers w ere used to ampli fy the tota l DN Af rom 28 soy bean accessions. The reult s show ed tha t repriducible amplified productscould be go t wi th 18 primers. The leng th o f amplificatio n product s are betw een 0. 6- 4.6kb. Totally 165 RAPD bands w ere identified, of which , 104 bands w ere commom in al lthe accessions and 61 bands w ere polymo rphic betw een any two accessions. The polymorphic bands accounted for 37% o f the to tal ampli fied ba nds. These primers could bedivided into six g roups. 1) no ampli ficatio n products; 2) no polymorphisms betw een amplifica tion products; 3) po lymorphic bands accounted fo r less tha n 15% of the totalbands; 4) polymo rphi sms betw een 15% - 30% ; 5) polymorphisms betw een 30% -50% ; 6) po lymo phi sms abov e 50% . Based on the g enetic distance mat rix, cluster analysi s w ere conducted. The result s show ed tha t these soybea n accessio ns co uld be div idedinto five g ro ups. Cultiv ars and lines clustered togeth er in a ny g roup o r subg roup w ereconsistent wi th thei r pedig ree and g eo graphical dist ributio n.

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更新日期/Last Update: 2017-01-13