[1]彭玉华　李卫.两个广谱抗虫基因源抗性背景DN A的多样性分析[J].大豆科学,1997,16(02):103-106.[doi:10.11861/j.issn.1000-9841.1997.02.0103]
　Peng Yuhua Li Wei.RESISTANT BACKGROUND DNA POLYMORPHISM BETWEEN TWO LEAF-FEEDINGINSECT RESISTANT SOYBEAN GERMPLASMS[J].Soybean Science,1997,16(02):103-106.[doi:10.11861/j.issn.1000-9841.1997.02.0103]
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两个广谱抗虫基因源抗性背景DN A的多样性分析

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第16卷 期数: 1997年02 页码: 103-106 栏目: 出版日期: 1997-05-20
Title:
RESISTANT BACKGROUND DNA POLYMORPHISM BETWEEN TWO LEAF-FEEDINGINSECT RESISTANT SOYBEAN GERMPLASMS
作者:
彭玉华　李卫
(中国农科院油料所,武汉430062)?
(湖北大学生物系武汉430062)
Author(s):
Peng Yuhua Li Wei
(Oil Crops Resear ch Institute , CAA S, 430062)?
(Dept . of L i f e Sci . Hubei Univ. 430062)
关键词:
大豆;  叶食性害虫;  近等基因系;  RAPDs
Keywords:
Soy bean ( G. max ) ;  Insect resistance;  NILs;  RAPDs
DOI:
10.11861/j.issn.1000-9841.1997.02.0103
文献标志码:
A
摘要:
利用8对抗- 感大豆叶食性害虫近似等位基因系, 100个随机引物进行PCR扩增,在406个稳定重现的片段中,有2个在近等基因系间表现多态性; 不同抗性来源的两组材料间的多态性DN A片段的有/无呈相反趋势,相同抗性来源的材料间的多态性DN A片段的有/无也存在差异。由此可以认为,抗性供体PI171451, PI229358的抗性背景DN A的多样性比较丰富,遗传背景相差较大,说明它们的抗性基因来源不同。在进行广谱抗虫育种实践中,为丰富育成品种,尤其是回交育成品种在抗性基因和抗性基因背景DN A上遗传多样性,避免其遗传脆弱性,以维持持久的抗性,建议同时将上两个广谱抗虫基因源用于大豆广谱抗虫育种计划。
Abstract:
8 pai rs o f leaf-feeding insect s resistant /susceptible so ybean near isogenic lines( NILs) a nd 100 randomly selected primers were inv olved in thi s RARDs study. Amo ngthe 406 repeatable DN A fragment s. 2 shew polymorphism betw een and among theN ILs /Pa rent s used in this prog ram. A div erse tendency o f the absence /presence o f the 2polymo rphic DN A f ragment s between the tw o g roups o f NILs f rom dif ferent do ner parentsimplied the possibility that PI171451 and PI2295358 ca rry di fferent leaf-feeding insects resi stance cont rolling gene( s) , and hav e di fferent DN A backg ro und. Therefore, equalinteg ra tion of leaf-feeding insect resista nce f rom PI171451 and PI229358 into soybean v arieties a re st rongly recommended.

参考文献/References:

[1 ]　S teven P Nol ting. 1989. Yi eld res ponse of s oybean of defoliat ion by th e M exican Bean Beetl e. J. Ent omology82( 4): 1212～ 1218

[2 ]　Van Duyn JW. 1971. Resis t ance in s oybeans to th e Mexican Bean Beetl e I. Sources of resis t ance. Crop Sci570～ 573
[3 ]　Hatch ett JH. 1976. Leaf-feeding resi s tance t o bollwo rm and t obacco b udw orm in th ree soybean plant int roductions.Crop Sci. 16: 277～ 280
[4 ]　Luedd ers VD 1977. Resis t ance of sel ect ed s oybean g enot ypes and s egret at ing populati ons to cab bage l ooperf eeding Crop Sci. 17: 395～ 396
[5 ]　Clark. 1972. Resis tance of certain s oyb ean cul tivars to bean l eaf beetl e, St riped bli s ter beet le, and bol lworm. J. Econ Ent om ol 65: 1669～ 1672
[6 ]　Ruf ener. 1989. Genetics of an tibosi s resis t ance to Mexi can Bean Beetl e in soybean. Crop Science 29: 618～621
[7 ]　Sis sion V A. 1976. Evidence of inh erit ance of resi s tance t o the Mexican Bean Beet le in Soybeans. Crop Science16: 835～ 837

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更新日期/Last Update: 2017-01-13