大豆磷脂酶D家族基因全基因组鉴定及耐盐性分析
- Title:
- Genome-wide Identification and Salt Tolerance Analysis of Phospholipase D Gene Family in Soybean
- 摘要:
- 磷脂酶D(Phospholipase D, PLD)及其产物磷脂酸(Phosphatidic Acid, PA)在植物生长发育和胁迫响应的调控中起着重要作用。目前，对大豆PLD基因家族的研究仍不够全面。为进一步寻找与大豆抗逆性相关的潜在候选基因，本研究利用全基因组分析方法对28个GmPLDs的基因结构、蛋白保守结构域、共线性和系统发育关系等进行研究，并采用qRT-PCR方法分析基因在盐胁迫下的表达量。结果表明：大豆PLD家族基因分布在16条染色体上，片段复制在大豆PLD基因家族的扩展中发挥了重要作用。基因表达模式分析显示GmPLDs在不同组织中的表达存在差异，启动子顺式作用元件分析显示GmPLDs具有参与非生物胁迫和激素途径的潜在功能。qRT-PCR分析显示，18个大豆PLDs基因表达受盐胁迫诱导显著上调，可能参与大豆耐盐胁迫。研究结果为解析及克隆大豆耐盐基因提供理论依据，并为挖掘响应非生物逆境胁迫GmPLDs基因提供了基础信息。
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