HUANG Wen-ting,FENG Nai-jie,ZHENG Dian-feng,et al.Effects of Uniconazole and Diethyl Aminoethyl Hexanoate on Photosynthetic Characteristics and Carbon Metabolism of Soybean Leaves[J].Soybean Science,2020,39(02):243-251.[doi:10.11861/j.issn.1000-9841.2020.02.0243]
烯效唑和胺鲜酯对大豆叶片光合特性与碳代谢的调控效应
- Title:
- Effects of Uniconazole and Diethyl Aminoethyl Hexanoate on Photosynthetic Characteristics and Carbon Metabolism of Soybean Leaves
- Keywords:
- Uniconazole; Diethyl aminoethyl hexanoate; Soybean; Photosynthetic characteristics; Carbon metabolism
- 文献标志码:
- A
- 摘要:
- 为探究化控技术对大豆盛花、盛荚和鼓粒期光合特性与碳代谢的调控效果，采用大田试验，于垦丰41初花期叶面喷施烯效唑（S3307）和胺鲜酯（DTA-6），以喷施清水作为对照，测定各生育时期光合参数、碳代谢产物及其相关酶、干物质和产量构成因素。结果表明：烯效唑和胺鲜酯能显著提高各生育时期的水分利用效率、净光合速率、SPAD、蔗糖与淀粉含量、及盛花期和盛荚期的气孔导度和蒸腾速率。胺鲜酯显著降低各生育时期转化酶活性，提高蔗糖合成酶与蔗糖磷酸合成酶活性，在盛荚期效果尤为显著，烯效唑可提高各生育时期蔗糖合成酶与蔗糖磷酸合成酶活性并降低花期转化酶活性。与对照相比，胺鲜酯和烯效唑提高盛花期叶和茎分配率，降低叶柄分配率，胺鲜酯显著提高各生育时期的单株干物质积累并降低盛荚期叶分配率，烯效唑显著提高盛花期单株干物质积累和盛荚期荚分配率。胺鲜酯和烯效唑可不同程度调控单株荚数、单株粒数和百粒重，提高产量，胺鲜酯和烯效唑的增产率分别达到8.78%和7.16%。说明烯效唑和胺鲜酯能够有效提高大豆各生育时期光合作用能力，促进碳代谢产物及相关酶的合成，有利于提高产量，且胺鲜酯作用效果最佳，增产率最高。
- Abstract:
- In order to explore the effects of chemical control technology on photosynthetic characteristics and carbon metabolism of soybean in flowering, podding and seed-filling stages, Kenfeng 41 was used as material to spray uniconazole（S3307）and diethyl aminoethyl hexanoate（DTA-6）on the leaves of soybean in early flowering stage, and clean water was sprayed as control. Photosynthetic parameters, carbon metabolites and their related enzymes, dry matter and yield components were measured at different growth stages. The results showed that: Uniconazole and diethyl aminoethyl hexanoate could significantly increase the water use efficiency, net photosynthetic rate, SPAD, the content of sucrose and starch in different growth stages and stomatal conductance and transpiration rate in flowering and podding stages. Diethyl aminoethyl hexanoate significantly decreased invertase activity and increased sucrose synthase and sucrose phosphate synthase activity at different growth stages, especially at podding stage. Uniconazole could increase sucrose synthase and sucrose phosphate synthase activity at different growth stages and reduce invertase activity at flowering stage. Compared with the control, uniconazole and diethyl aminoethyl hexanoate increased the distribution rate of leaves and stems, decreased the distribution rate of petioles at flowering stage, diethyl aminoethyl hexanoate significantly increased the dry matter accumulation per plant in different growth stages and decreased the distribution rate of leaves at podding stage, uniconazole significantly increased the dry matter accumulation per plant and the distribution rate of pods at podding stage.Uniconazole and diethyl aminoethyl hexanoate could regulate pod number per plant, seed number per plant and 100-seed weight in varying degrees to increase yield. The yields of diethyl aminoethyl hexanoate and uniconazole increased by 8.78% and 7.16%, respectively. In conclusion, uniconazole and diethyl aminoethyl hexanoate can effectively improve the photosynthetic capacity of soybean at different growth stages, promote the synthesis of carbon metabolites and related enzymes, and it’s help for improving yield. Diethyl aminoethyl hexanoate has the best effect and the highest yield.
参考文献/References:
［1］Athow K L.Soybean pest management［J］. Journal of The American Oil Chemists′ Society,1981,58(3):130-135.［2］文莲莲,李岩,张聃丘,等.冬季温室补光时长对番茄幼苗生长、光合特性及碳代谢的影响［J］.植物生理学报,2018,54(9):1490-1498. (Wen L L, Li Y, Zhang D Q, et al. Effects of supplemental light duration on the growth, photosynthetic characteristic and carbon metabolism of tomato seedlings in winter under solar greenhouse［J］. Plant Physiology Journal, 2018, 54(9): 1490-1498.)［3］Hamed K, Ghorban K. The role of uniconazole in improving physiological and biochemical attributes of bean (Phaseolus vulgaris L.) subjected to drought stress［J］. Journal of Crop Science and Biotechnology, 2019, 22(2): 161-168. ［4］Yuan L, Xu D Q. Stimulatory effect of exogenous GA3 on photosynthesis and the level of endogenous GA1+3 in soybean leaf［J］. Journal of Plant Physiology and Molecular Biology, 2002, 28(4): 317-320. ［5］Ahmad I, Kamran M, Yang X N, et al. Effects of applying uniconazole alone or combined with manganese on the photosynthetic efficiency, antioxidant defense system, and yield in wheat in semiarid regions［J］. Agricultural Water Management,2019,25(2):400-414.［6］齐德强,赵晶晶,冯乃杰,等.烯效唑(S3307)和胺鲜酯（DTA-6）对马铃薯叶与块茎糖代谢及产量的影响［J］.作物杂志, 2019(4):148-153. (Qi D Q, Zhao J J, Feng N J, et al. Effects of S3307 and DTA-6 on sugar metabolism and yield of potato leaves and tubers［J］. Crops, 2019(4): 148-153.)［7］温国泉,刘永贤,农梦玲,等.不同植物生长调节剂对南方淮山药叶片光合作用的影响［J］.西南农业学报,2017,29(7): 1590-1594. (Wen G Q, Liu Y X, Nong M L, et al. Effects of plant growth regulators (combination) on leaf photosynthesis of Yam in South China［J］.Southwest China Journal of Agricultural Sciences, 2017, 29(7): 1590-1594.)［8］王畅,赵海东,冯乃杰,等. S3307和DTA-6对芸豆生殖生长阶段光合特性和产量的影响［J］.草业学报,2018,27(11): 162-170. (Wang C, Zhao H D, Feng N J, et al. Effects of S3307 and DTA-6 on the photosynthetic characteristics and yield of kidney bean plants in the reproductive stage［J］. Acta Prataculturae Sinica, 2018, 27(11): 162-170.)［9］Liu C J, Feng N J, Zheng D F, et al. Uniconazole and diethyl aminoethyl hexanoate increase soybean pod setting and yield by regulating sucrose and starch content［J］. Journal of The Science of Food and Agriculture, 2019, 99(2): 748-758.［10］潘彬荣,岳高红,刘永安,等.胺鲜酯对甜玉米叶片光合特征、籽粒糖分积累和产量的调控效应［J］. 农药学学报, 2015, 17(6): 660-666. (Pan B R, Yue G H, Liu Y A, et al. Regulation effects of diethyl aminoethyl hexanoate on the leaf photosynthetic characteristics, the grain sugar accumulation and the yield of sweet corn［J］. Chinese Journal of Pesticide Science, 2015, 17(6): 660-666.)［11］郑殿峰,赵玖香,赵黎明.植物生长调节剂对大豆光合作用和同化物分配的影响［J］.西南农业学报, 2008, 21(5): 1265-1269. (Zheng D F, Zhao J X, Zhao L M. Effect of plant growth regulator(PGRs) on photosynthesis and assimilate distribution of soybean［J］. Southwest China Journal of Agricultural Sciences,2008,21(5):1265-1269.)［12］蒋莲芝,樊亚娟,刘俊环,等.大豆应用多效唑试验效果初探［J］.大豆通报, 2001(5):6. ( Jiang L Z, Fan Y J,Liu J H,et al.Preliminary study on the effect of paclobutrazol application in soybean［J］.Soybean Bulletin,2001(5):6.)［13］周天,胡勇军,周晓梅,等.三十烷基磷酸酯钾对大豆的生理功能及产量的影响［J］.东北师大学报(自然科学),2004,36(2): 31-33.(Zhou T, Hu Y J, Zhou X M, et al. Effect of potassium hydrogen 1-triacontyl phosphate (TPK) on the physiological function and yield of soybean［J］. Journal of Northeast Normal University (Natural Science), 2004, 36(2): 31-33.)［14］王永锋,裴桂英,张跃进,等.不同微生物肥和调节剂在大豆上的施用效果［J］.安徽农业科学,2001,29(4):509-510.(Wang Y F,Pei G Y,Zhang Y J,et al. Preliminary report on micro-fertilizer and moderator applied in soybean［J］. Journal of Anhui Agricultural Sciences,2001,29(4):509-510.)［15］张宪政.作物生理研究法［M］.北京:农业出版社,1992. (Zhang Z X. Crop physiology research method［M］. Beijing: Agricultural Press, 1992.)［16］张志良.植物生理学实验指导［M］.北京:高等教育出版社,2001. (Zhang Z L. Guidance of plant physiology experiments［M］. Beijing: Higher Education Press, 2001.)［17］Yadav S K, Singh V, Jyothi L N, et al.Carbohydrates and sucrose metabolizing enzymes in the leaves of Vigna mungo genotypes as influenced by elevated CO2 concentration［J］.Journal of Agricultural Science and Technology,2013,15 (6) :1107-1120.［18］Rodriguez R E, Debernardi J M, Palatnik J F. Morphogenesis of simple leaves: Regulation of leaf size and shape［J］.Wiley Interdisciplinary Reviews: Developmental Biology, 2014, 3(1): 41-57. ［19］叶子飘,胡文海,闫小红,等.基于光响应机理模型的不同植物光合特性［J］.生态学杂志, 2016, 35(9): 2544-2552. (Ye Z P, Hu W H, Yan X H, et al. Photosynthetic characteristics of different plant species based on a mechanistic model of light-response of photosynthesis［J］.Chinese Journal of Ecology, 2016, 35(9): 2544-2552.)［20］杨文钰,樊高琼,任万君,等.烯效唑干拌种对小麦光合作用和14C同化物分配的影响［J］.作物学报,2005,31(9):1173-1178.(Yang W Y, Fan G Q, Ren W J, et al. The effects of uniconazole waterless seed dressing on photosynthesis and 14C assimilate distribution in wheat［J］.Acta Agronomica Sinica,2005,31(9):1173-1178.)［21］许淑娟,孙周平,王志鑫.烯效唑对雾培马铃薯光合特性及荧光的影响［J］.西北农业学报,2009,18(3):127-130.(Xu S J,Sun Z P,Wang Z X. Effects of uniconazole treatments on photosynthetic and physiological indices of aeroponic potato［J］.Acta Agriculturae Boreali-Occidentalis Sinica,2009,18(3):127-130.)［22］郭会君,靳文奎,赵林姝,等.实践八号卫星飞行环境中不同因素对小麦的诱变效应［J］.作物学报,2010(5): 764-770. (Guo H J, Jin W K, Zhao L S, et al. Mutagenic effects of different factors in spaceflight environment of Shijian-8 satellite in wheat［J］.Acta Agronomica Sinica,2010(5): 764-770.)［23］许乃霞,杨益花,苏祖芳.抽穗后水稻株型与高光效群体形成关系的研究［J］.耕作与栽培,2009(5): 17-19. (Xu N X,Yang Y H,Su Z F. Study on the relation between plant type and population formation of high photosynthetic efficiency in rice after heading［J］.Tillage and Cultivation,2009(5): 17-19.) ［24］夏淑芳,沈允钢,李德耀,等.光合作用及其产物的转化、积累与输出［C］.中国科学院植物生理研究所,中国科学院植物研究所.光合作用研究进展(第三卷).北京:科学出版社,1984:156. (Xia S F, Shen R G, Li D Y, et al. Photosynthesis and the transformation, accumulation and export of its products［C］.Institute of Plant Physiology, Chinese Academy of Sciences, Institute of Botany, Chinese Academy of Sciences. Advances In Photosynthesis (Volume 3). Beijing: Science Press, 1984:156.)［25］邓忠,白丹,翟国亮,等.不同植物生长调节剂对新疆棉花干物质积累、产量和品质的影响［J］.干旱地区农业研究,2011,29(3):122-127.(Deng Z, Bai D, Zhai G L, et al. Study on the effect of plant growth regulators on dry matter accumulation, yield and quality of cotton in Xinjiang［J］.Agricultural Research in the Arid Areas,2011,29(3):122-127.)［26］王宝生,刘春娟,冯乃杰,等.植物生长调节剂对大豆植株上、中部干物质积累及产量的影响［J］.南方农业学报,2015,46(9):1567-1573.(Wang B S, Liu C J,Feng N J,et al.Effect of plant growth regulator on dry matter accumulation in upper and central parts and yield of soybean［J］.Journal of Southern Agriculture,2015,46(9):1567-1573.)［27］Zeeman S C,Smith S M, Smieh A M.The diurnal metabolism of leaf starch［J］.Biochemical Journal,2007,401(1):13-28.［28］郑绍辉, 赵鑫, Md. Matiul Islam. 大豆鼓粒期的氮积累及转移对产量的影响［J］. 土壤与作物,2017, 6(1): 1-8. (Zheng S H, Zhao X, Md.Matiul Islam.Influence of nitrogen accumulation and redistribution on seed yield in soybean during seed filling［J］.Soils and Crops,2017,6(1):1-8.)［29］Jones T L.Chilling delays circadian pattern of sucrose phosphate synthase and nitrate reductase activity in tomato［J］.Plant Physiology,1998,118:149-158.［30］Sarquís J I, Gonzalez H, de Jiménez E S, et a1. Physiological traits associated with mass selection for improved yield in a maize population［J］.Field Crops Research,1998,56:239-246.［31］张玉平,刘强,荣湘民,等.不同水稻品种(组合)碳代谢关键酶活性比较［J］.中国稻米,2007(4):15-19.(Zhang Y P, Liu Q, Rong X M, et al. Comparison of key enzyme activities in carbon metabolism of different rice varieties (combinations)［J］.China Rice,2007(4):15-19.)［32］张盼盼,王小林,郭亚宁,等.胺鲜酯浸种对苗期糜子形态和叶片生理特性的影响［J］.草业学报,2018,27(9):77-84.(Zhang P P,Wang X L,Guo Y N,et al. Effects of DTA-6 seed soaking on morphological and leaf physiological characteristics in proso millet at seedling stage［J］.Acta Prataculturae Sinica,2018,27(9):77-84.)［33］宋春艳,冯乃杰,郑殿峰,等.植物生长调节剂对大豆叶片碳代谢相关生理指标的影响［J］.干旱地区农业研究,2011,29(3):91-95. (Song C Y,Feng N J,Zheng D F,et al. Effects of plant growth regulators(PGRs)on carbon metabolism related indicators in soybean leaves［J］.Agricultural Research in the Arid Areas,2011,29(3):91-95.)
相似文献/References:
[1]罗庆明,杨文钰,曹晓宁,等.大豆苗期叶片形态对烯效唑干拌种的响应[J].大豆科学,2009,28(06):1004.[doi:10.11861/j.issn.1000-9841.2009.06.1004]
LUO Qing-ming,YANG Wen-yu,CAO Xiao-ning,et al.Response of Leaf Morphological Characters of Soybean Seedlings to Uniconazole Treatment for Dry Seed[J].Soybean Science,2009,28(02):1004.[doi:10.11861/j.issn.1000-9841.2009.06.1004]
[2]宋胜,冯乃杰,郑殿峰.烯效唑浸种对大豆种子萌发及保护性酶系的影响[J].大豆科学,2008,27(02):259.[doi:10.11861/j.issn.1000-9841.2008.02.0259]
SONG Sheng,FENG Nai-jie,ZHENG Dian-feng.Effect of Seed Soaking with Uniconazole on Germination and Anti- oxidantEnzyme of Soybean[J].Soybean Science,2008,27(02):259.[doi:10.11861/j.issn.1000-9841.2008.02.0259]
[3]龚万灼,张正翼,杨文钰,等.烯效唑干拌种对大豆形态特征和产量的影响[J].大豆科学,2007,26(03):369.[doi:10.3969/j.issn.1000-9841.2007.03.017]
GONG Wan-zhuo,ZHANG Zheng-yi,YANG Weng-yu,et al.EFFECT OF UNICONAZLOE FOR DRY SEED TREATMENT ON MORPHOLOGICAL CHARACTERISTICS AND YIELD OF SOYBEAN[J].Soybean Science,2007,26(02):369.[doi:10.3969/j.issn.1000-9841.2007.03.017]
[4]闫艳红,万燕,杨文钰,等.叶面喷施烯效唑对套作大豆花后碳氮代谢及产量的影响[J].大豆科学,2015,34(01):75.[doi:10.11861/j.issn.1000-9841.2015.01.0075]
YAN Yan-hong,WAN Yan,YANG Wen-yu,et al.Effect of Spraying Uniconazole on Carbon and Nitrogen Metabolism and Yield of Relay Strip Intercropping Soybean[J].Soybean Science,2015,34(02):75.[doi:10.11861/j.issn.1000-9841.2015.01.0075]
[5]张春初 王永锋 裴桂英 马赛飞 郭光 张跃进 孙志安.烯效唑在大豆上的应用效果[J].大豆科学,2002,21(02):151.[doi:10.11861/j.issn.1000-9841.2002.02.0151]
Zhang Chunchu Wang Yongfeng Pei Guiying Ma Saifei Guo Guang Zhang Yaojin Sun Zhian.EFFECTOF PENTEFEZO APPLYINGON THE SOYBEAN[J].Soybean Science,2002,21(02):151.[doi:10.11861/j.issn.1000-9841.2002.02.0151]
[6]张明会,王碧莹,李 双,等.根瘤菌与烯效唑互作对大豆生长及固氮的影响[J].大豆科学,2018,37(01):112.[doi:10.11861/j.issn.1000-9841.2018.01.0112]
ZHANG Ming-hui,WANG Bi-ying,LI Shuang,et al.Effect of Uniconazole and Rhizobium Interaction on Growth and Nitrogen Fixation in Soybean[J].Soybean Science,2018,37(02):112.[doi:10.11861/j.issn.1000-9841.2018.01.0112]
[7]梁建秋,于晓波,张明荣,等.化控和密度对套作大豆南黑豆20农艺性状和产量的影响[J].大豆科学,2018,37(06):876.[doi:1011861/jissn1000-98412018060876]
LIANG Jian-qiu,YU Xiao-bo,ZHANG Ming-rong,et al.Effect of Various Chemical Treatments and Density on Agronomic Traits and Yield of Inter-planting Soybean Cultivar Nanheidou 20[J].Soybean Science,2018,37(02):876.[doi:1011861/jissn1000-98412018060876]
[8]王通宇,方淑梅,王庆燕,等.叶面喷施不同化控复配剂对大豆产量与品质的影响[J].大豆科学,2023,42(01):70.[doi:10.11861/j.issn.1000-9841.2023.01.0070]
备注/Memo
收稿日期：2019-09-10