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[1]魏圣军,耿殿祥,邬奇,等.UV-A辐射对大豆芽苗菜中抗坏血酸含量的影响[J].大豆科学,2015,34(03):420-426.[doi:10.11861/j.issn.1000-9841.2015.03.0420]
　WEI Sheng-jun,GENG Dian-xiang,WU Qi,et al.Effect of UV-A Irradiation on Ascorbic Acid Content in Soybean Sprout[J].Soybean Science,2015,34(03):420-426.[doi:10.11861/j.issn.1000-9841.2015.03.0420]

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UV-A辐射对大豆芽苗菜中抗坏血酸含量的影响

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第34卷期数: 2015年03期页码: 420-426 栏目: 出版日期: 2015-06-25

Title:: Effect of UV-A Irradiation on Ascorbic Acid Content in Soybean Sprout

作者:: 魏圣军; 耿殿祥; 邬奇; 张晓燕; 苏娜娜; 崔瑾; 南京农业大学生命科学学院，江苏南京 210095

Author(s):: WEI Sheng-jun; GENG Dian-xiang; WU Qi; ZHANG Xiao-yan; SU Na-na; CUI Jin; College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China

关键词:: 大豆芽苗菜; UV-A; 抗坏血酸; DHAR; GR

Keywords:: Soybean sprout; UV-A; Ascorbic acid; DHAR; GR

DOI:: 10.11861/j.issn.1000-9841.2015.03.0420

文献标志码:: A

摘要:: 以黑暗培养为对照，以白光、红光、蓝光、黄光、UV-A和UV-B为处理，研究了不同光质对大豆芽苗菜生长和抗坏血酸含量的影响。结果表明：与对照相比，光照处理显著降低了大豆芽苗菜下胚轴的长度（白光除外），且显著提高了下胚轴和子叶中抗坏血酸含量。与其它光质处理相比，UV-A连续光照36 h后，下胚轴和子叶中抗坏血酸含量提高最显著。进一步着重研究了UV-A的调节机理，与对照相比，UV-A连续光照36 h后，大豆芽苗菜子叶中谷胱甘肽含量显著升高，下胚轴和子叶中DHAR、GR酶活性及其基因的相对表达量均显著提高。综上所述，光照有利于大豆芽苗菜中抗坏血酸含量的积累；UV-A可能是通过提高DHAR、GR酶活性及基因的表达量显著提高抗坏血酸的含量。

Abstract:: Effects of light quality on the growth and ascorbic acid content of soybean sprouts were studied in this article.Dark treatment was used as the control, and white, red, blue, yellow, UV-A and UV-B irradiation were used as different treatments. The results showed that compared with the dark, light reduced the hypocotyl length of soybean sprouts except white light, and more importantly the content of ascorbic acid were significantly improved both in hypocotyl and cotyledon of soybean sprouts.Compared with other light qualities, the most striking increase in contents of ascorbic acid in hypocotyl and cotyledon of soybean sprouts were happened after continuous UV-A illumination for 36 h. Further study was emphasized on the regulation mechanism of UV-A about how it could significantly raise the content of ASA, the results indicate that, after UV-A 36 h continuous illumination, the content of GSH in cotyledon, the enzymes activities of DHAR and GR and their gene relative expression in hypocotyl and cotyledon were notability improved in contrast to the control.To sum up, light is conductive to the accumulation of ascorbic acid in soybean sprouts; and UV-A through strengthen the enzyme activity of DHAR and GR and up-regulating their gene-expression to increase the content of ascorbic acid.

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备注/Memo

基金项目：国家自然科学基金（31171998）；国家基础人才培养基金（J1310015）。

第一作者简介：魏圣军（1989-），男，硕士，主要从事植物光生物学方面的研究。 E-mail: 2012116010@njau.edu.cn。

通讯作者：崔瑾(1974-)，女，博士，教授，主要从事设施栽培环境调控机理研究。 E-mail: cuijin@njau.edu.cn。

更新日期/Last Update: 2015-07-15