YUAN Xiang-yang,GUO Ping-yi,ZHANG Li-guang,et al.Effect of Glyphosate on Photosynthesis Diurnal Variation of Glyphosate-resistant Soybean [Glycine max (L.) Merr.][J].Soybean Science,2011,30(03):419-423.[doi:10.11861/j.issn.1000-9841.2011.03.0419]
草甘膦对抗草甘膦大豆光合特性日变化的影响
- Title:
- Effect of Glyphosate on Photosynthesis Diurnal Variation of Glyphosate-resistant Soybean [Glycine max (L.) Merr.]
- 文章编号:
- 1000-9841(2011)03-0419-05
- Keywords:
- Glyphosate; Glyphosate-resistant soybean; Photosynthetic characteristics; Diurnal variation
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 在大田条件下，采用随机区组设计，研究了不同用量草甘膦对抗草甘膦大豆（RR1）光合特性日变化的影响。结果表明：（1）在未喷施草甘膦情况下，抗草甘膦大豆（RR1）的净光合速率（Pn）、蒸腾速率（Tr）和气孔导度（Gs）日变化均呈单峰曲线，峰值分别出现在10∶00、12∶00和10∶00。RR1叶片叶绿素含量指数（CCI）的日变化呈先降低后升高趋势，14∶00左右最低。水分利用效率（WUE）和胞间CO2浓度（Ci）随着时间的推移均呈波浪式变化，WUE在6∶00、10∶00和16∶00有3个小峰，而Ci在6∶00最高。（2）喷施草甘膦后，RR1光合特性的日变化趋势总体与未喷药前相似。Pn和CCI随草甘膦用量的增加呈降低趋势；但当草甘膦用量大于4.48 L·hm-2时，CCI和Pn显著下降。12∶00以前（包含12∶00），除低用量（1.12 L·hm-2）的草甘膦促进RR1的Gs外，各用量抑制了RR1的Tr和Gs；而12∶00以后，草甘膦却促进了RR1的Tr和Gs。草甘膦增加了RR1的Ci，而大于2.24 L·hm-2的草甘膦却降低了RR1的WUE。表明光强和草甘膦会影响大豆的光合特性和叶绿素含量。
- Abstract:
- Random complete block design was used to research the different dosages of glyphosate on diurnal variation of photosynthetic characteristics in leaves of glyphosate-resistant soybean (RR1) in the field. The results showed that: (1) The trend of diurnal variation of net photosynthetic rate (Pn), transpiration rate (Tr) and stomatal conductance (Gs) in RR1 leaves showed a single peak curve, the peak appeared at about 10∶00, 12∶00 and 10∶00, respectively. Chlorophyll content index (CCI) declined until 14∶00, then increased. The water use efficiency (WUE) and intercellular CO2 concentration (Ci) of RR1 changed like wave as time went on， WUE appeared three peaks at 6∶00, 10∶00 and 16∶00, respectively, while the highest Ci was appeared at 6∶00. (2) The trend of diurnal variation of photosynthetic characteristics in RR1 leaves treated with glyphosate was similar with the control. Pn and CCI decreased with increasing of glyphosate dosage, but only dropped significantly when glyphosate dose > 4.48 L·hm-2. Glyphosate dose at 1.12 L·hm-2 promoted Gs of RR1, while the others inhibited Tr and Gs before 12∶00 (including 12∶00). Glyphosate increased Tr and Gs of RR1 after 12∶00. Ci of RR1 increased with increasing of glyphosate dosage, and glyphosate > 2.24 L·hm-2 reduced WUE of RR1. Results suggest photosynthetic characteristics and chlorophyll content of RR1 leaves are affected by photosynthetic active radiation and glyphosate.
参考文献/References:
[1]Reddy K N. Glyphosate-resistant soybean as a weed management tool: Opportunities and challenges[J]. Weed Biology and Management, 2002,1(4):193-202. [2]Nelson K A, Renner K A. Cost-effective weed management in wide and narrow-row glyphosate resistant soybean[J]. Journal of Production Agriculture,1999,12:460-465. [3]Nelson K A, Renner K A. Soybean growth and development as affected by glyphosate and post emergence herbicide tank mixtures[J]. Agronomy Journal,2001,93(2):428-434. [4]原向阳, 郭平毅, 张丽光, 等. 不同时期喷施草甘膦对大豆生理指标的影响[J]. 中山大学学报（自然科学版）,2009,48(2):90-94. (Yuan X Y, Guo P Y, Zhang L G, et al. Impact of spraying glyphosate on physiological index of soybean at different growth stages[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni(Natural Science Edition),?2009,48(2):90-94.) [5]原向阳, 张丽光, 郭平毅, 等. 第一复叶期喷施农达41%水剂对大豆生理指标的影响[J]. 中国生态农业学报, 2008, 16(2):401-404. (Yuan X Y, Zhang L G, Guo P Y, et al. Impact of Roundup 41%aqua on physiological index of soybean at one-trifoliolate leaf stage [J]. Chinese Journal of Eco-Agriculture,2008,16(2):401-404.) [6]Weber M L, Kapusta G. Control of tall weeds in soybean with glyphosate[J]. Research Report of the North Central Weed Science Society,1998,55:371-373. [7]Young B G, Young J M. Influence of late season post-emergence herbicides on soybean growth and development [J]. Research Report of the North Central Weed Science Society,2000,57:263-264. [8]Zablotowicz R M, Krishna N R. Impact of glyphosate on the Bradyrhizobium japonicum?symbiosis with glyphosate-resistant transgenic soybean: A mini-review[J]. Journal of Environmental Quality,2004,33:825-831. [9]Zablotowicza R M, Krishna N R. Nitrogenase activity, nitrogen content and yield responses to glyphosate in glyphosate-resistant soybean[J]. Crop Protection,2007,26(3):370-376. [10]King C A, Purcell L C, Earl D V. Plant growth and nitrogenase activity of glyphosate-tolerant soybean in response to foliar glyphosate applications[J]. Agronomy Journal,2001,93:179-186. [11]原向阳, 郭平毅, 张丽光, 等. 干旱胁迫下草甘膦对抗草甘膦大豆幼苗保护酶活性及脂质过氧化作用的影响[J]. 中国农业科学, 2010,43(4): 698-705. (Yuan X Y, Guo P Y, Zhang L G, et al. Glyphosate and post-drought rewatering on protective enzyme activities and membrane lipid peroxidation in leaves of glyphosate-resistant soybean [Glycine max?(L.) Merr.] seedlings [J]. Scientia Agricultura Sinica, 2010,43(4):698-705.) [12]原向阳, 毕耀宇, 王鑫, 等. 除草剂对抗草甘膦大豆光合作用和蒸腾作用的影响[J]. 农业现代化研究, 2006,27(4):311-313. (Yuan X Y, Bi Y Y, Wang X, et al. Effect of herbicides on photosynthesis and transpiration of glyphosate-resistant soybean [J]. Research of Agricultural Modernization,2006,27(4):311-313.) [13]高辉远, 邹琦, 程炳嵩. 大豆光合日变化的不同类型及其影响因素[J]. 大豆科学,1992,11(3):219-225. (Gao H Y, Zou Q, Cheng B S. The different types of diurnal course of photosynthesis of soybean [Glycine max?(L). Merr.] leaves and related factors [J]. Soybean Science,1992,11(3):219-225.) [14]高辉远, 邹琦, 程炳嵩. 大豆光合日变化与内生节奏的关系(简报)[J]. 植物生理学通讯, 1992,28(4):262-264. (Gao H Y, Zou Q, Cheng B S. Relationship between diurnal variation of photosynthesis and circadian rhythm in soybean leave [J]. Plant Physiology Communication,1992,28(4):262-264.) [15]邹冬生, 郑丕尧, 王瑞舫. 大豆叶片光合、蒸腾的日变化及其与生态因子的相关性研究[J]. 植物生态学与植物学学报,1990,14(4):350-357. (Zou D S, Zheng P Y, Wang R F. Studies on the diurnal variation of the photosynthesis and transpiration of soybean leaf and its correlation with ecological factors in field conditions [J]. Acta Phytoecologica Geobotanica Sinica,1990,14(4): 350-357.) [16]郑国生, 邹琦. 不同天气条件下田间大豆光合作用日变化的研究[J]. 中国农业科学,1993,26(1):44-50. (Zheng G S, Zou Q. A study on the diurnal variation of photosynthesis of field-grown soybean in different weather conditions [J]. Scientia Agricultura Sinica, 1993,26(1):44-50.) [17]原向阳, 郭平毅, 张丽光, 等.草甘膦对大豆光合特性日变化的影响[J]. 山西农业大学学报(自然科学版),2010,30(6):500-504. (Yuan X Y, Guo P Y, Zhang L G, et al. Effect of glyphosate on diurnal variation of photosynthesis in leaves of glyphosate-resistant soybean [Glycine max?(L.) Merr.] [J].Journal of Shanxi Agricultural University (Natural Science Edition),2010,30(6):500-504.) [18]高辉远, 邹琦, 程炳嵩. 大豆光合日变化过程中气孔限制和非气孔限制的研究[J]. 西北植物学报,1993,13(2):96-102. (Gao H Y, Zou Q, Cheng B S. Study on the stomatal and nonstomatal limitation during the diurnal course of photosynthesis in soybean leaves [J]. Acta Botanica Boreali-Occidentalla Sinica,1993,13(2):96-102.) [19]张瑞朋, 杨德忠, 傅连舜, 等. 不同来源大豆品种光合速率日变化及其影响因子的研究[J]. 大豆科学,2007,26(4):490-495. (Zhang R Y, Yang D Z, Fu L S, et al. Research of photosynthesis diurnal variation and its affecting factors for different source soybeans [J].Soybean Science,2007,26(4):490-495.) [20]郑国生, 王焘. 田间冬小麦叶片光合午休过程中的非气孔限制[J]. 应用生态学报,2001,12(5):799-800. (Zheng G S, Wang T. Nonstomatic limitations in midday depression of photosynthesis in winter wheat leaves[J]. Chinese Journal of Applied Ecology, 2001,12(5):799-800.) [21]何春霞，李吉跃，张燕香，等. 五种绿化树种叶片比叶重、光合色素含量和δ13C的开度与方位差异[J]. 植物生态学报,2010,34(2):134-143. (He C X, Li J Y, Zhang Y X, et al. Differences in leaf mass per area, photosynthetic pigments and δ13C by orientation and crown position in five greening tree species[J]. Chinese Journal of Plant Ecology,2010,34(2):134-143.) [22]周忆堂，马红群，梁丽娇，等.不同光照条件下长春花的光合作用和叶绿素荧光动力学特征[J].中国农业科学, 2008, 41(11): 3589-3595. (Zhou Y T, Ma H Q, Liang L J, et al. Photosynthetic characteristics and chlorophyll fluorescence in leaves of Catharanthus roseus?grown under different light intensities[J].Scientia Agricultura Sinica, 2008,41(11):3589-3595.)
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备注/Memo
基金项目：引进国际先进农业科学技术计划“948计划”资助项目（61002）；山西农业大学2009年引进人才科研启动项目（XB2009018）;山西省研究生优秀创新资助项目（20081063）。