[1]刘琪,相世刚,胡玉琪,等.野生大豆对草甘膦的耐性筛选及施用草甘膦后叶绿素荧光参数的变化[J].大豆科学,2018,37(03):399-407.[doi:10.11861/j.issn.1000-9841.2018.03.0399]
　LIU Qi,XIANG Shi-gang,HU Yu-qi,et al.Tolerance Screening of Wild Soybean to Glyphosate and the Changes of Chlorophyll Fluorescence Parameters after Glyphosate Application[J].Soybean Science,2018,37(03):399-407.[doi:10.11861/j.issn.1000-9841.2018.03.0399]
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野生大豆对草甘膦的耐性筛选及施用草甘膦后叶绿素荧光参数的变化

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第37卷 期数: 2018年03期 页码: 399-407 栏目: 出版日期: 2018-05-20
Title:
Tolerance Screening of Wild Soybean to Glyphosate and the Changes of Chlorophyll Fluorescence Parameters after Glyphosate Application
作者:
刘琪; 相世刚; 胡玉琪; 强胜; 宋小玲
（南京农业大学 生命科学学院/杂草研究室，江苏 南京 210095）
Author(s):
LIU Qi; XIANG Shi-gang; HU Yu-qi; QIANG Sheng; SONG Xiao-ling
(Weed Research Laboratory,College of Life Science of Nanjing Agricultural University,Nanjing 210095,China)
关键词:
野生大豆; 草甘膦; 叶绿素荧光参数; 耐性
Keywords:
Wild soybean;  Glyphosate;  Chlorophyll fluorescence parameters;  Tolerant
DOI:
10.11861/j.issn.1000-9841.2018.03.0399
文献标志码:
A
摘要:
为研究不同种群野生大豆对草甘膦耐性的差异以及草甘膦对其叶绿素荧光参数的影响，在温室条件下测定了17个不同地理位置种群野生大豆幼苗对草甘膦的耐性，从中筛选出了对草甘膦耐性最高、较高、较低以及最低的种群，并测定了这4个种群在喷施草甘膦后叶绿素荧光参数的变化。结果表明：根据50%有效量（ED50）值，湖南郴州（CZ）种群耐性最低，辽宁铁岭（TL）种群耐性较低，安徽舒城（SC）种群耐性较高，江苏金坛（JT）种群耐性最高，耐性指数为分别为1，1.78，2.17，3.17。在施用不同剂量草甘膦后2 d，4个种群的PSⅡ最大光化学效率（Fv/Fm）、PSⅡ实际光合效率（Y(Ⅱ)）、表观电子传递效率（ETR）、光化学荧光淬灭系数（qP）总体上均随着草甘膦剂量的增加而降低。非光化学淬灭系数（NPQ）呈现不同的变化趋势，JT种群NPQ随着草甘膦剂量的增加而逐渐升高， SC、TL及CZ种群则呈现先增加后下降的趋势。总体上耐性越高的种群Fv/Fm、Y(Ⅱ)、ETR、qP在草甘膦处理后下降幅度越小，NPQ越能够维持稳定上升。在施用1.2~2.4,0.6,0.6~1.8,0.6~1.2 kg·hm-2有效剂量草甘膦后，JT种群Fv/Fm、Y（Ⅱ）、ETR、qP分别显著高于其它种群。因此，可通过比较不同野大豆种群喷施草甘膦后叶绿素荧光参数的差异进行野生大豆对草甘膦耐性的快速筛选。
Abstract:
In order to study the difference of glyphosate tolerance of wild soybean from different populations and the effects of glyphosate on their fluorescence parameters, the glyphosate tolerance of different wild soybean populations which were sampled from 17 different sites were measured in greenhouse, and 4 populations with the highest, higher, lower, and lowest tolerance to glyphosate were selected to determine the changes of chlorophyll fluorescence parameters after spraying glyphosate. The results showed that, based on ED50 value, the population of Jintan (JT), Shucheng (SC), Tieling (TL) and Chenzhou (CZ) had the highest, higher, lower, and lowest tolerance to glyphosate and their tolerance index was 3.17, 2.17, 1.78 and 1, respectively. Two days after applying different doses of glyphosate, the maximal photochemical efficiency (Fv/Fm) and actual photochemical efficiency (Y(Ⅱ)) of PSⅡ, photochemical quenching (qP) and electron transport rate (ETR) of those 4 populations were generally decreased with the increasing glyphosate does. The non-photochemical quenching (NPQ) showed different trend of change in different populations, the NPQ of JT population increased while the NPQ of SC, TL and CZ population increased first and then decreased with the increasing glyphosate does. Consequently, after glyphosate application, the higher the tolerance, the smaller decline rate of Fv/Fm, Y (II), ETR and qP, and the more steady increasing of the NPQ.After the application of 1.2-2.4,0.6,0.6-1.8 and 0.6-1.2 kg·ha-1 active ingredients of glyphosate, the Fv/Fm,Y(Ⅱ), ETR and qP of JT population were significantly higher than that of other populations respectively.Therefore, after glyphosate application, comparing the difference of fluorescence parameters of different wild soybean populations could be used for rapid screening of glyphosate tolerance.

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备注/Memo

收稿日期：2018-03-09

基金项目：国家转基因生物新品种培育重大专项(2016ZX08012005-006)。
第一作者简介：刘琪（1993-），男，硕士，主要从事转基因生物安全评估研究。E-mail:liuqi88@126.com。
通讯作者：宋小玲（1969-），女，博士，教授，主要从事转基因生物安全评估研究。E-mail:sxl@njau.edu.cn。

更新日期/Last Update: 2018-06-08