[1]王宇,张锴,孙伟明,等.冀东野生大豆对大豆花叶病毒的抗性鉴定及抗病反应[J].大豆科学,2017,36(01):92-97.[doi:10.11861/j.issn.1000-9841.2017.01.0092]
　WANG Yu,ZHANG Kai,SUN Wei-ming,et al.Resistance Identification and Response of Wild Soybean (Glycine soja) to Soybean Mosaic Virus in Eastern Hebei Province[J].Soybean Science,2017,36(01):92-97.[doi:10.11861/j.issn.1000-9841.2017.01.0092]
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冀东野生大豆对大豆花叶病毒的抗性鉴定及抗病反应

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第36卷 期数: 2017年01期 页码: 92-97 栏目: 出版日期: 2017-01-20
Title:
Resistance Identification and Response of Wild Soybean (Glycine soja) to Soybean Mosaic Virus in Eastern Hebei Province
作者:
王宇1; 张锴2; 孙伟明2; 王帅1; 曹红梅1; 董秋平2; 张华雪2; 李凯3
河北科技师范学院 园艺科技学院，河北 秦皇岛 066000; 2.河北科技师范学院 生命科技学院，河北 秦皇岛 066000; 3.南京农业大学 国家大豆改良中心/作物遗传与种质创新国家重点实验室，江苏 南京 210095
Author(s):
WANG Yu1;  ZHANG Kai2;  SUN Wei-ming2;  WANG Shuai1;  CAO Hong-mei1;  DONG Qiu-ping2;  ZHANG Hua-xue2;  LI Kai3
1.College of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; 2. Life Science and Technology Institute, Hebei Normal University of Science and Technology, Qinhuangdao 066000, China; 3. National Center for Soybean Improvement of Nanjing Agricultural University/National Key Laboratory for Crop Genetics and Germplasm Enhancement，Nanjing 210095, China
关键词:
野生大豆; 大豆花叶病毒; 抗性鉴定; 基因表达
Keywords:
Wild soybean(Glycine soja);  Soybean Mosaic Virus;  Resistance identification;  Gene expression
DOI:
10.11861/j.issn.1000-9841.2017.01.0092
文献标志码:
A
摘要:
对冀东地区收集的162份野生大豆（Glycine soja）材料进行大豆花叶病毒（SMV）抗性鉴定并对筛选到的抗、感材料植株体内的过氧化物酶（POD）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）活性及病程相关基因GmPR-1和GmPR-10的表达进行测定。结果表明：抗病野生大豆材料7份（4.3%），中抗材料6份（3.7%），中感材料62份（38.3%），感病材料87份（53.7%）；抗病材料POD和CAT活性比对照显著增加，而感病材料显著降低，抗病材料的GmPR-1和GmPR-10基因表达量比对照显著增加，感病材料与对照无显著差异，初步表明POD、CAT及病程相关基因GmPR-1和GmPR-10可能与大豆抗病性有关。
Abstract:
In this study, the resistance identification of 162 wild soybean (Glycine soja) to Soybean Mosaic Virus (SMV) and the enzymatic activity of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) and genes expression of GmPR1 and GmPR10 in resistance/susceptible wild soybean plants were investigated. The results showed that 7 wild soybean materials were resistance accessions(4.3%), 6 materials were moderately resistance accessions (3.7%), 62 materials were intermediate accessions (38.3%) and 87 materials were susceptible accessions (53.7%). The activity of POD and CAT in resistance plants were significantly increased compared to CK, whereas the activity of these two enzymes were significantly decreased in susceptible plants, the expression of GmPR1and GmPR10 in resistance plants were significantly increased compared to susceptible plants. Preliminarily showed that POD, CAT and GmPR1 and GmPR10 may be related to resistance response in soybean-SMV interaction.

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备注/Memo

基金项目：河北省自然科学基金（C2016407069）；河北省高校科学技术研究项目（QN2015097）；国家自然科学基金（31671718）；中央高校基本科研业务费（Y0201600115）；国家现代农业产业技术体系（CARS-04）。

第一作者简介：王宇（1986-），女，硕士，主要从事大豆抗病育种研究。E-mail: Wangyu66yuanyi@163.com。
通讯作者：李凯（1979-），男，博士，副教授，从事大豆抗病育种研究。E-mail:Kail@njau.edu.cn。

更新日期/Last Update: 2017-03-14