[1]孙家骥,李爽,朱晓峰,等.胞囊线虫侵染抗感大豆品种后根系谷胱甘肽代谢水平差异研究[J].大豆科学,2016,35(03):468-471.[doi:10.11861/j.issn.1000-9841.2016.03.0468]
　SUN Jia-ji,LI Shuang,ZHU Xiao-feng,et al.Glutathione Metabolism Different Expression in Ｒesistant and Susceptible SoybeanＲoots after Infected by Heterodera glycines Ichinohe[J].Soybean Science,2016,35(03):468-471.[doi:10.11861/j.issn.1000-9841.2016.03.0468]
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胞囊线虫侵染抗感大豆品种后根系谷胱甘肽代谢水平差异研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 468-471 栏目: 出版日期: 2016-05-20
Title:
Glutathione Metabolism Different Expression in Ｒesistant and Susceptible SoybeanＲoots after Infected by Heterodera glycines Ichinohe
作者:
孙家骥; 李爽; 朱晓峰; 王媛媛; 陈立杰; 刘晓宇; 陈井生; 段玉玺
沈阳农业大学植物保护学院/北方线虫研究所，辽宁沈阳110866
Author(s):
SUN Jia-ji; LI Shuang; ZHU Xiao-feng; WANG Yuan-yuan; CHEN Li-jie; LIU Xiao-yu; CHEN Jing-sheng; DUAN Yu-xi
College of Plant Protection，Shenyang Agricultural Uinversity /Plant Protection Station of Liaoning Province，Shenyang 110866，China
关键词:
灰皮支黑豆;  谷胱甘肽;  大豆胞囊线虫;  荧光定量PCＲ
Keywords:
Huipizhiheidou;  Glutathione;  Heterodera glycines Ichinohe;  Ｒeal-time Quantitative PCＲ
分类号:
S565. 1
DOI:
10.11861/j.issn.1000-9841.2016.03.0468
文献标志码:
A
摘要:
以大豆胞囊线虫3 号生理小种抗性品种灰皮支黑豆( ZDD2315) 和感病品种辽豆15 为材料，室内人工接种大豆胞囊线虫，检测抗、感品种侵染后二龄幼虫数量变化及根内活性氧变化，利用Ｒeal-Time Quantitative PCＲ 分析了在大豆胞囊线虫3 号生理小种互作下谷胱甘肽代谢途径相关基因在抗感大豆品种根内的相对表达量变化情况。结果表明: 接种后第5 天，抗感品种根内幼虫的数量大致相同; 第15 天，在灰皮支黑豆中，根内线虫幼虫数量减少; 辽豆15根内H2O2含量始终略高于灰皮支黑豆; 大豆胞囊线虫侵染后，谷氨酰胺半胱氨酸连接酶基因接种后3 个时期的表达均上调; 而谷胱甘肽合成酶基因在感病品种和抗病品种中处理组呈现了不同的表达趋势，尤其在接种15 d，在抗病品种灰皮支黑豆中表达下调，而在感病对照中表达上调; H 型谷胱甘肽合成酶基因在抗性品种灰皮支黑豆中下调，说明谷胱甘肽相关基因的减少和下调在抗病品种灰皮支黑豆对大豆胞囊线虫的抗性呈正相关，导致大豆根系的氧化性增强，从而不利于大豆胞囊线虫的定殖和发育。
Abstract:
The resistant variety Huipizhiheidou and susceptible cultivar Liaodou 15 were tested against soybean cyst nematode( Heterodera glycines，SCN) . The plants were inoculated with eggs of SCN 3 to test the number of the second stage juvenilesand the content of reactive oxygen species. The expression of Glutathion synthesis pathway related genes were detected in theresistant Huipizhiheidou and the susceptible Liaodou 15 by Ｒeal-time Quantitative PCＲ. The results showed that at 5 dpi，nodifference of J2 occurred between Huipizhiheidou and Liaodou 15，at 15 dpi，the number of juveniles invaded roots of HuipizhiHeidouwas less than Liaodou 15. Quantification of reactive species( ＲOS) was higher in Liaodou 15. The relative expressionlevel of glutathione glutamate cysteine ligase was higher in Huipizhiheidou and Liaodou 15 after all inoculated times. Incontrast，the expression of GSHS and hGSHS was significantly lower in Huipizhiheidou than in Liaodou 15 at 15 days. The reductionof glutathione related genes were positively related with resistance of Huipizhiheidou，lead to the oxidation of soybeanroot，which go against to the development of soybean cyst nematode

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备注/Memo

基金项目: 国家自然科学基金( 31171569 ) ; 国家现代农业产业技术体系建设专项( CAＲS-04-PS13 ) ; 农业部公益性行业科研专项( 201503114) ; 教育部博士点基金( 20112103110006) 。

第一作者简介: 孙家骥( 1990-) ，男，硕士，主要从事大豆胞囊线虫研究。E-mail: sunjiaji007@ foxmail. com。
通讯作者: 段玉玺( 1964-) ，男，教授，博导，主要从事植物病理学、植物线虫学研究。E-mail: duanyx6407@163. com。

更新日期/Last Update: 2016-07-24