[1]林志伟,于春生,李海燕,等.哈茨木霉抗多菌灵突变株对大豆菌核病的诱导抗性生理[J].大豆科学,2016,35(03):472-476.[doi:10.11861/j.issn.1000-9841.2016.03.0472]
　LIN Zhi-wei,YU Chun-sheng,LI Hai-yan,et al.Induced Ｒesistance Physiological on Stem Ｒot of Soybean by Carbendazim ＲesistanceMutant Strain of Trichoderma harzinaum[J].Soybean Science,2016,35(03):472-476.[doi:10.11861/j.issn.1000-9841.2016.03.0472]
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哈茨木霉抗多菌灵突变株对大豆菌核病的诱导抗性生理

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第35卷 期数: 2016年03期 页码: 472-476 栏目: 出版日期: 2016-05-20
Title:
Induced Ｒesistance Physiological on Stem Ｒot of Soybean by Carbendazim ＲesistanceMutant Strain of Trichoderma harzinaum
作者:
林志伟1; 于春生2; 李海燕1; 孙冬梅1; 李敏3; 张匀华4
1. 黑龙江八一农垦大学农学院，黑龙江大庆163319;?
2. 林口县农技推广中心，黑龙江牡丹江157600;?
3. 东华理工大学学院，江西南昌330013;?
4. 黑龙江省农业科学院植物保护研究所，黑龙江哈尔滨150086
Author(s):
LIN Zhi-wei; YU Chun-sheng; LI Hai-yan; SUN Dong-mei; LI Min; ZHANG Yun-hua
1. Heilongjiang BaYi Agricultural University，Daqing 163319，China;?
2. Linkou County Agricultural Technology Extension Center，Mudanjiang 157600，China;
3. East China Institute of Technology，Nanchang 330013，China;?
4. Institute of Plant Protection，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，China
关键词:
哈茨木霉;  抗多菌灵突变株;  大豆菌核病;  诱导抗性
Keywords:
Trichoderma harzianum;  Carbendazim resistant mutant strain;  Sclerotinia sclerotiorum;  Induced resistance
DOI:
10.11861/j.issn.1000-9841.2016.03.0472
文献标志码:
A
摘要:
通过测定接种菌核病菌后不同时间大豆植株抗逆酶活性的变化，研究了土壤中施用拮抗真菌哈茨木霉菌及其抗多菌灵突变株对植株抗逆生理的影响。结果表明: 哈茨木霉突变菌株的处理对SOD 酶的诱导出现较早，在接种4 h 后酶活开始增强，而接种后36 h 相比对照可提高67%以上; 接种72 h 后POD 酶活比对照提高27. 3%; 而MDA 含量与对照相比，却始终处于相对较低水平。表明哈茨木霉菌抗多菌灵突变株的应用具有诱导植株抗逆酶活性提高及增强植株抗病能力的作用。
Abstract:
By measuring the plant enzyme activity changes of soybean after inoculate Sclerotinia sclerotiorum on the soybeanleaves， the effects of applying antagonism fungi，Trichoderma harzianum and its carbendazim resistant mutant strain，to the soilon plant physiology was studied. Ｒesult showed that. The induced SOD enzyme activity occurred earlier in the treatment of T.harzianum mutant strain，the increasing occurred after inoculated S. sclerotiorum for 4 hours，after inoculated for 36 hours theSOD enzyme activity increased 67% than control，the POD enzyme activity increased 27. 3% than control，but the MDA contentwas always lower than control. These indicated that T. harzianum and its carbendazim resistant mutant strain could beused to improve the induced enzyme activity and disease resistance.

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备注/Memo

基金项目: 农业部公益项目( 201103016-03A1) ; 国家自然科学基金地区科学基金( 21267001) 。

第一作者简介: 林志伟( 1970-) ，男，硕士，副教授，主要从事植物病虫害防治研究。E-mail: lzwsdm@ sohu. com。

更新日期/Last Update: 2016-07-24