TAO Bo,PAN Si-yang,WANG Huan,et al.Effect of UV Mutation on Chlorimuron-ethyl Degradation Ability ofAspergillus Niger[J].Soybean Science,2016,35(03):455-460.[doi:10.11861/j.issn.1000-9841.2016.03.0455]
紫外诱变对黑曲霉降解氯嘧磺隆能力的影响
- Title:
- Effect of UV Mutation on Chlorimuron-ethyl Degradation Ability ofAspergillus Niger
- 关键词:
- 紫外( UV) 诱变; 氯嘧磺隆除草剂; 黑曲霉; 微生物降解; 高效液相色谱
- Keywords:
- UV; Herbicide Chlorimuron-ethyl; Aspergillus Niger; Microbial degradation; HPLC
- 分类号:
- Q939. 96; S143. 92
- 文献标志码:
- A
- 摘要:
- 采用紫外( UV) 诱变方法对黑曲霉进行诱变,用高效液相色谱法测定菌株对氯嘧磺隆的降解率,系统研究了黑曲霉经紫外诱变后对氯嘧磺隆降解能力的影响。结果表明: 经紫外( UV) 诱变黑曲霉总变异率随UV 强度和辐照时间的不同而不同,受时间影响较大,对黑曲霉最佳的紫外( UV) 诱变条件为UV 强度30 W、辐照时间150 s,此条件下黑曲霉的正变率为35. 16%; 紫外( UV) 诱变可以显著提高黑曲霉降解氯嘧磺隆的速度,在UV 强度30 W、时间150 s 时黑曲霉对氯嘧磺隆的降解速度最大,氯嘧磺隆半衰期为7. 76 h 比原始菌株( 24. 86 h) 缩短17. 1 h。最终筛选出降解能力提高最显著的菌株H1,其降解能力比原始菌株提高73. 16%,且连续培养10 代后降解率均在89% 左右,降解性能及生长速度稳定,可以稳定遗传。
- Abstract:
- In this paper,the effect of ultraviolet mutagenesis on chlorimuron-ethyl degradation ability of Aspergillus Niger wassystematic studied. Ultraviolet( UV) method is used to Aspergillus Niger mutagenesis,and a high performance liquid chromatographymethod was established for determination of the degradation rates of stain to chlorimuron-ethyl. The result showedthat,induced mutation by ultraviolet( UV) the total aberration rates of Aspergillus Niger was varied with the UV intensity andirradiation time,greatly influenced by time,the best UV condition was 30 W and 150 s,positive mutation rate was 35. 16%.The degradation rates of herbicide chlorimuron-ethyl by Aspergillus Niger was studied after UV mutagenesis. UV mutagenesiscould significantly improve degradation rates of herbicide chlorimuron-ethyl by Aspergillus Niger,When the UV condition was30 W and 150 s the degradation rate of Aspergillus Niger on chlorimuron-ethyl was the greatest,half-life of chlorimuron-ethylshortened 17. 1 h than before. Finally selected the most significant effect strains H1,its degradation ability increased by73. 16% than the original strains,and continued training after 10 generations degradation of performance and growth rate werestable and could be stable genetic.
参考文献/References:
[1] 邹月利,陶波. 除草剂降解真菌对氯嘧磺隆的降解作用[J].土壤通报,2013,44 ( 6) : 1445-1448. ( Zou Y L,Tao B. Researchon the effects of different strains on the degradation of herbicidechlorimuron-ethyl[J]. Chinese Journal of Soil Science,2013,44( 6) : 1445-1448. )
备注/Memo
基金项目: 国家自然科学基金( 30971834) 。